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We narrowed to 257 results for: PORA

Showing: 61 - 80 of 257 results

  1. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...pronuclei of fertilized eggs, which leads to random incorporation of that sequence into the zygote’s genome, a...unpredictable. Transposons, however, efficiently incorporate into the zygote’s genome after injection into...Sandoval-Villegas N, Nurieva W, Amberger M, Ivics Z (2021) Contemporary Transposon Tools: A Review and Guide through...

  2. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...Desktop Genetics .For ssODN design, remember to incorporate PAM-blocking mutations (e.g. convert the NGG ...into cells. Optimization of delivery methods (electroporation, lipofection etc) is the most crucial aspect...includes selection of transfection buffer and electroporation pulse program to achieve high transfection ...

  3. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ... DNA to favor the edit Once the prime editor incorporates the edit into one strand, there’s a mismatch...MutSα–MutLα mismatch repair (MMR) complex, to temporarily inhibit this process and promote copying of the...it from hybridizing with the unedited strand. Temporarily inhibiting this complex may allow time for 5′...

  4. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...containing these base editors Spatially and temporally regulated AAV-based genome editing In 2016, Jonathan...AAV-based genome editing system is regulated not only temporally with doxycycline, but it can also be regulated...

  6. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...the Cre recombinase, are used to spatially and temporally control transgene expression. Lox sites are directional...sites lets researchers control the spatial and temporal activation of a transgene. Figure 2: FLEX...

  7. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...(Addgene #104102 - #104105) using in utero electroporation or with AAVs (prepared with Addgene #104110...Figure 3) that can be introduced using in utero electroporation. Then at your preferred time points, sacrifice...

  8. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...salt causes arcing during electroporation which would cause the electroporation to fail). When incubating...

  9. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...visits an institution to perform research there temporarily. The length of stay could be from a month to ...own. This will include, but is not limited to temporary housing, transportation, living expenses, and ...

  10. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...when performing amplifications, and we use electroporation to ensure a good transformation efficiency....library, Addgene usually does four to eight electroporation reactions using a total of ~400ng of library...

  12. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...permanently integrate into the host genome or temporarily express whatever it encodes. This ability to ...straightforward across applications, while electroporation of a human, for example, isn’t a reasonable...

  14. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...): These are considered low copy vectors and incorporate part of an ARS along with part of a centromere...nutrient, the host cells will die unless they have incorporated the plasmid carrying the required gene.  The...

  15. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...your gRNA. Pro tip: modifications are typically incorporated at the termini of the crRNA molecule (usually...Ramel, MC., et al. Photoswitchable gRNAs for Spatiotemporally Controlled CRSIPR-Cas-based Genomic Regulation...

  17. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...system allows scientists to both spatially and temporally knock-down proteins of interest.  Plasmids for...intersectional strategy using a split intein for refined spatiotemporal transgene control in Caenorhabditis elegans...

  18. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...upon these original tools that allows for both spatiotemporal protein degradation and quantification of this...expressed under a desired promoter with a specific spatiotemporal expression pattern and an auxin inducible degron...

  19. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...interest. However, FACS is unable to spatially and temporally target single cells. Now, Francois St-Pierre’...way to isolate individual cells with unique spatiotemporal phenotypes from large heterogeneous cultures...

  20. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...ncRNA localization and function, but directly incorporating very long ncRNAs is impractical. Instead, tags...nuclease-dead Cas9 protein (dCas9) and a sgRNA to temporarily block the transcription machinery of a target...
Showing: 61 - 80 of 257 results