We narrowed to 257 results for: PORA

...containing these base editors Spatially and temporally regulated AAV-based genome editing In 2016, Jonathan...AAV-based genome editing system is regulated not only temporally with doxycycline, but it can also be regulated...

...   Timing Optogenetics offers a high degree of temporal resolution as receptors can be turned on and off... meet these requirements. TL;DR If precise spatiotemporal control of a small subset of neurons is necessary...

...salt causes arcing during electroporation which would cause the electroporation to fail). When incubating...

...the Cre recombinase, are used to spatially and temporally control transgene expression. Lox sites are directional...sites lets researchers control the spatial and temporal activation of a transgene. Figure 2: FLEX...

...(Addgene #104102 - #104105) using in utero electroporation or with AAVs (prepared with Addgene #104110...Figure 3) that can be introduced using in utero electroporation. Then at your preferred time points, sacrifice...

...proteins.  For computer-savvy users looking to incorporate FPbase data in their own projects and applications... and elaboration.  Current projects include incorporation of biosensors, protein evolution lineages, 2...

...visits an institution to perform research there temporarily. The length of stay could be from a month to ...own. This will include, but is not limited to temporary housing, transportation, living expenses, and ...

...when performing amplifications, and we use electroporation to ensure a good transformation efficiency....library, Addgene usually does four to eight electroporation reactions using a total of ~400ng of library...

...permanently integrate into the host genome or temporarily express whatever it encodes. This ability to ...straightforward across applications, while electroporation of a human, for example, isn’t a reasonable...

...your gRNA. Pro tip: modifications are typically incorporated at the termini of the crRNA molecule (usually...Ramel, MC., et al. Photoswitchable gRNAs for Spatiotemporally Controlled CRSIPR-Cas-based Genomic Regulation...

...nucleolytically inactive Cas9-VP64 fusion; 2. A sgRNA incorporating two MS2 RNA aptamers at the tetraloop and stem-loop...size of the backbone, transformations require electroporation. References & Further Reading: For more ...

...): These are considered low copy vectors and incorporate part of an ARS along with part of a centromere...nutrient, the host cells will die unless they have incorporated the plasmid carrying the required gene.  The...

...and the G protein (supplied in trans) will be incorporated into the viral envelope (but not the viral genome...B. Retrograde monosynaptic tracing reveals the temporal evolution of inputs onto new neurons in the adult...

...ncRNA localization and function, but directly incorporating very long ncRNAs is impractical. Instead, tags...nuclease-dead Cas9 protein (dCas9) and a sgRNA to temporarily block the transcription machinery of a target...

...viral Rev gene from the packaging plasmid and incorporating it into a fourth plasmid. In addition, the promoter...third generation packaging systems, this system incorporates the Tet-off transcriptional activation system...

...upon these original tools that allows for both spatiotemporal protein degradation and quantification of this...expressed under a desired promoter with a specific spatiotemporal expression pattern and an auxin inducible degron...

...interest. However, FACS is unable to spatially and temporally target single cells. Now, Francois St-Pierre’...way to isolate individual cells with unique spatiotemporal phenotypes from large heterogeneous cultures...

...introduce modifications at the DNA level in a temporally and locally defined manner. This allows scientists...injection Viral vectors allow a tight spatial and temporal control of gene expression manipulation. They ...

...system allows scientists to both spatially and temporally knock-down proteins of interest.  Plasmids for...intersectional strategy using a split intein for refined spatiotemporal transgene control in Caenorhabditis elegans...

...(1, 3). The gRNA can be further modified by incorporation of an RNA hairpin, such as the MS2 hairpin, ...with nuclease-competent Cas9. An RNA hairpin incorporated into the truncated gRNA recruits a transcriptional...