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We narrowed to 205 results for: PREP

Showing: 61 - 80 of 205 results
  1. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...CRISPRko sgRNA library as pooled library or lentiviral prep! Dolcetto: human CRISPRi sgRNA library While CRISPRko...Dolcetto library! Now also available as a lentiviral prep. Calabrese: human CRISPRa sgRNA library In addition...Find the Calabrese pooled library and lentiviral prep here! Applications for the Root and Doench CRISPR...
  2. Hot Plasmids - January 2023

    Type
    Blog Post
    ...subset of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles...pitch a Hot Plasmids section (antibodies and viral preps welcome!) here. Here's what you'll find in this...
  3. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...channel. And with no more ado, the questions!  AAV Prep and Technique Q1: Is there any way to deliver a ...you think it is possible to make an efficient AAV prep for a 4.2 kB insert with the CMV promoter using ...
  4. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...though we do provide a few non-CRISPR related viral preps. Cas9 is the headliner of most CRISPR applications...lentivirus. Secondly, you can use a LentiCas9-Blast viral prep to create a single stable Cas9-expressing cell line...
  5. Addgene: The First Twenty Years

    Type
    Blog Post
    ...ready-to-use viral vector preps. Today, that number has grown to over 114,600 viral preps. And in 2025, we’ll...
  6. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...ccdB SurvivalTM, one can efficiently propagate and prep plasmids containing the ccdB gene, which can then... strains could possibly be used to propagate and prep your ccdB-containing empty backbones but should ...
  7. Overcoming the Challenges of Lentiviral Production

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    Blog Post
    ...considerably lower volume than with the unconcentrated prep. For example, at Addgene we typically concentrate...opposed to the 15 ml collected from an unconcentrated prep. With this in mind, if the experiment requires ...
  8. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    Blog Post
    ... available at Addgene, either as in-stock viral preps or through our Packaged on Request service. Alternatively...for each serotype below) and make your own viral prep. However, tissue tropism varies both between species...AAV Packaged On Request Browse all in-stock AAV preps Browse all AAV plasmids ...
  9. Plasmids 101: Colony PCR

    Type
    Blog Post
    ... cultures with the clones you pick and only mini-prep the positive ones. Regardless of which method you... a few positive clones, the last step is to mini-prep these clones and submit the plasmids for Sanger ...
  10. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...eliminate any HEK-293T cells. If you titrate your viral preps, lentiviral titration requires an additional couple...Lentivirus Production Tips for Titering Your Lentivirus Preps More resources on Addgene.org Addgene's virus protocols...
  11. How to Be an Excellent Trainee

    Type
    Blog Post
    ...In research there is a saying that if the sample prep is poor, then the data will be poor. The same is...assistants, and sitting out on the bench waiting for you, prep for research work has to be done by the researchers...
  12. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...submissions will receive a 20% off coupon for a viral prep. And if you've used the Data Hub to help select ...
Showing: 61 - 80 of 205 results