...pMD2.G for producing our ready-to-use lentivirus preps. You can find our lentivirus production protocol...

...excellent Q&A from Tim Miles on everything from AAV prep to targeting to animal models. It was one of our...

... cultures with the clones you pick and only mini-prep the positive ones. Regardless of which method you... a few positive clones, the last step is to mini-prep these clones and submit the plasmids for Sanger ...up colony PCR reactions is nearly identical to preparing a standard PCR reaction: combine template, primers...other colony PCR tips are highlighted below. Preparing template: Pick a single colony with a sterile ...

... AAV preps are affordable, available in small volumes, and ready to ship. All viral vector preps are purified...and viral genome sequencing (VGS). In-stock AAV preps are distributed as 100 µL solutions of purified ...reported with each shipment. If you can't find the prep you need, try Addgene's AAV Packaged on Request ... pages above. You can also do a search of viral preps and filter by the serotype needed....

...In research there is a saying that if the sample prep is poor, then the data will be poor. The same is...assistants, and sitting out on the bench waiting for you, prep for research work has to be done by the researchers...highlight how to prepare and things to keep an eye out for during training. Preparation before bench work...the training. In this blog we will review the preparation and planning you can do to build a bench mentor-mentee... training in the lab! If you don’t do proper preparation and pay attention during training, then you won... you – so do your best to show up as your most prepared, can-do self and make the most of it! So, what... schedule, be respectful of theirs! Show up prepared and take training seriously, as reviewed above...

...eliminate any HEK-293T cells. If you titrate your viral preps, lentiviral titration requires an additional couple...Lentivirus Production Tips for Titering Your Lentivirus Preps More resources on Addgene.org Addgene's virus protocols...transfection). This differs from typical insect cell preparations, which we talk about below. Processing The viral...

...experimental details on the spreadsheet: Name your viral prep Enter the specific names of the plasmids you plan...goes, “failing to plan is planning to fail.” Preparation is a key step to any experiment and can help ...

...plasmid you didn't have to grow the bacteria and prep the plasmid before you begin your cloning experiment...Great news. A subset of our collection has been prepared as part of our new cloning grade DNA service and...plasmid without spending time and resources in preparative experiments.  We’re excited to begin offering...etc. and/or directly long term storage. Every preparation is quality controlled through next generation...

...submissions will receive a 20% off coupon for a viral prep. And if you've used the Data Hub to help select ...

...started a new service distributing ready-to-use viral preps in 2016 and we just reached our 10,000th viral vector...

...plasmid from us or receive a ready-to-use viral preparation from the inventory below. Lentiviruses are distributed...

...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...Sternson 154867 pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA hM4D(Gi) - Inhibition mCherry fusion Flp-dependent...* Gether 154868 pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA hM3D(Gq) - Activation mCherry fusion Flp-dependent...

...We provide high quality AAV preps from select plasmids in the repository. Browse our control AAV collection...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

...We provide high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...table and filters below to find ready-to-use AAV preparations of optogenetics plasmids available in Addgene's...

...We provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of...AAV Browse the following tables for in-stock AAV preps that encode site-specific recombinases, which can...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

...Lentivirus Get tips for titering your lentiviral preps Resources on Addgene.org: Check out the lentivirus...lentivirus guide Find ready-to-use lentiviral preps at Addgene ...expression using other methods.  In terms of preparation, VSV-G is stable and can withstand ultracentrifugation...

...Antibody of the Day!  Figure 4: The plasmid, viral prep, and antibody of the day While those are the biggest...

...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...final viral vector preparation. In some cases, in vivo testing of these viral preparations was performed by...Addgene is producing ready-to-use viral vector preparations in a retrograde serotype from the Alla Karpova...David Schaffer laboratories. These viral vector preparations were produced with the rAAV2-retro helper plasmid...DREADD Roth 154867 pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA Syn Inhibitor, Flp-dependent DREADD Gether 154868...154868 pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA Syn Activator, Flp-dependent DREADD Gether 83896 pAAV-hDlx-GiDREADD-dTomato-Fishell...

...order to generate high quality preparations. After production, all virus preps are titered and subjected to...typically prepared using endotoxin-free plasmid purification kits. Resources Browse lentiviral preps available... was a gift from Karl Deisseroth (Addgene viral prep # 55632-AAVrg). pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2...Jaws-KGC-GFP-ER2] was a gift from Edward Boyden (Addgene viral prep # 84445-AAVrg). Electron Microscopy The ratio of...arrowhead). Scale bar = 100 nm. Resources Browse AAV preps available from Addgene. After receiving your virus...plasmid encoding adenoviral helper sequences. AAV preparations are purified by iodixanol gradient ultracentrifugation...purity and titers adequate for in vivo studies. Preparations are then aliquoted and stored at -80 °C. Titer...