Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 736 results for: RAN-1

Showing: 61 - 80 of 736 results

  1. Retrovirus Plasmids

    Type
    Collection
    ...1774 for neomycin selection. Bob Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression with...into multiple plasmids for safety: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked... the transgene fused to eGFP. Hans Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression...and transfer plasmids. Viral... Viral Plasmids Gamma-Retroviral Plasmids Gamma-Retroviral Plasmids Transfer Plasmids...encoded on RNA. The genome includes a reverse transcriptase and an integrase to insert the viral genome ...can take advantage of this to stably integrate transgenes of interest into a variety of cell types. Wild-type...

  2. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...details on how they work!) Figure 1: Base transitions are edits between the purine bases (adenine... inducing targeted DNA transversions in human cells. Nature Biotechnology, 39(1), 41–46. https://doi.org...R-loop structure. In order to bind DNA, Cas9 requires (1) a guide RNA, (2) a complementary DNA sequence, and...given the pithier name CGBE1, for “C → G Base Editor 1,” and had a C → G editing efficiency of up to 68%... the CGBE: DNA polymerase D2 (POLD2), exonuclease 1 (EXO1), and RNA binding motif protein X-linked (RBMX...excision repair proteins. Nature Communications, 12(1), 1384. https://doi.org/10.1038/s41467-021-21559-9...machine learning methods. Nature Communications, 12(1), 4902. https://doi.org/10.1038/s41467-021-25217-y...

  3. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

    Type
    Blog Post
    ...activity in single cells using translocation reporters. Nature protocols, 13 1, 155-169. PMID: 29266096. Find...Kinases: they regulate many proteins, with ~1/3 of human proteins predicted to be phosphorylated on at...which have previously been characterized. See Box 1 in Kudo et al. for more details on the design of the...phosphatase-accessible. Kinase Translocation Reporters (KTRs) Kinase Translocation Reporters (KTRs) are genetically...particularly important for regulating signal transduction and measuring kinase activity at the single-...provides an alternative tool with their Kinase Translocation Reporters (KTRs) whose cellular localization...activity is enhanced, leading to cytoplasmic translocation of the reporter. As a result, the KTR is shuttled...

  4. Caltech Systemic Capsids

    Type
    Collection
    ... Caltech and can help guide your decision. Figure 1: Decision tree for choosing your AAV capsid. Your ...tdTomato Control Boyden 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Fishell 99130 pAAV-mDlx-NLS-mRuby2....AX with GRE or transgenic animal Oligodendrocytes - AAV-PHP.eB with GRE or transgenic animal Brain vascular....AX with GRE or transgenic animal L5 & Inhibitory - AAV-PHP.eB with GRE or transgenic animal L2/3 & L4...AAV.CAP-B10 with GRE or transgenic animal Excitatory - AAV.PHP.N with GRE or transgenic animal For PNS applications... pAAV-S5E2-GFP-fGFP E2 regulatory element GFP, membrane-targeted GFP Control Dimidschstein 192552 pAAV-CAG... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

  5. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...Table 1 has a detailed list of the bacterial species these plasmids are compatible with.  Table 1: Non-...Figure 1: Idealized examples of a narrow host range plasmid with only one ori and a broad host range plasmid...Improved broad-host-range plasmids for DNA cloning in Gram-negative bacteria. Gene, 70(1), 191–197. https... N.J.), 765, 327–343. https://doi.org/10.1007/978-1-61779-197-0_19  Meyer, R. (2009). Replication and ...?  Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Tranfection...define plasmid host range, identify a few of the features that confer broad host range in plasmids in nature...features to create broad host range recombinant vectors.  A plasmid’s host range is determined by the different...

  6. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...138920210790886871 Pray, L. (2008) Transposons: The jumping genes. Nature Education 1(1):204. https://www.nature.com...reverse transcriptase activities.    Figure 1: Overview of retrotransposon transposition. Retrotransposons...are also known as retrotransposons. They transpose by a ‘copy-paste’ mechanism (Fig. 1). They first copy...their host’s genome.   There are two types of Class 1 TEs: those with long terminal repeats (LTRs) and those...CRISPR-Cas system. This system has four major components: 1) a CRISPR RNA (crRNA), 2) four proteins (TniQ, Cas8...express a transposase or reverse transcriptase so they can transpose. Both class I and II transposons can be...eukaryotic genomes.  Components of a transposon system Transposon: The transposon is the DNA sequence that moves...

  7. Viral Vector Guides and Plasmids

    Type
    Collection
    ...integration. Introduction to Viral Vectors Figure 1: Wild-type virus propagation and use of viral plasmids...packaging capacity, high transduction efficiency, and high but transient transgene expression.They can induce...130 nm Dividing Yes Moderate AAV Transient or Stable* Single-stranded linear DNA ∼4.5 kb 18–26 nm Dividing.../Non-dividing No* Very Low Adenoviral Transient Double-stranded linear DNA 5–36 kb 105 nm Dividing/Non-dividing...of viral vectors and find viral production and transfer plasmids for a wide array of research purposes...are versatile tools because of their ability to transduce dividing and non-dividing cells. These virusal...immunogencity. These viral vectors can direct long-term transgene expression, but generally don't integrate into...

  8. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ... find a particular viral spike protein (MERS, CoV-1, CoV-2), modification, or variant. ID Plasmid Description...provirus and the overall strength of viral transcriptional response in these cells. Warner Greene 105621...

  9. Antibody Production

    Type
    Collection
    ...formulation buffer is phosphate-buffered saline containing 1 mM sodium azide. This buffer is not compatible for...extracted from cells, separated by SDS-PAGE, transferred to a membrane, and labeled with the antibody and a species-specific...expression vectors, human embryonic kidney cells are transfected with the plasmid(s) encoding the heavy and light...anti-GFP antibody), the target antigen is first transiently expressed in mammalian cells. To learn which ... The fluorescence pattern is compared to an untransfected control. Immunohistochemistry (IHC) Tissue sections...antibody. Protein expression is compared to an untransfected control and the protein size is confirmed. Residual...contain low levels of co-purified plasmid from transfection. This residual plasmid is extracted and amplified...

  10. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...cell surface proteins. J Am Chem Soc. 127(37):12770-1. PMID:16159249...with a recognizable sub-cellular localization (membrane, nucleus, etc.) are also available Non-toxic to... is based on the human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein, and is suitable...Vector SNAP-tag Empty backbone for stable and transient mammalian expression 101133 pSNAP-CaaX Control...Control Plasmid SNAP-tag Control for endomembrane (CaaX box) localization 101129 pSNAPf-Cox8A Control Plasmid...Vector CLIP-tag Empty backbone for stable and transient mammalian expression 101134 pCLIPf-H2B Control...pMCP-tag (m) Vector ACP/MCP-tag Empty backbone for transient mammalian expression 101126 pACP-tag (m)- 2 Vector...

  11. Distribution to Industry

    Type
    Collection
    ... your account. We will notify you by email within 1 business day regarding your account verification. ... Material Transfer Agreements Most Addgene orders must be accompanied by a material transfer agreement...industry scientists. Please note this does not guarantee availability but lets us know what plasmids would...agreement (MTA), including transfers to for-profit entities. The MTA is a legal agreement between the recipient... the depositing institution(s). It governs the transfer of biological materials and intellectual property...Please see the Sample Agreements on our Technology Transfer Information page for more information. To learn...

  12. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    ... compounds with various antimicrobial activities (1). She has developed a fast endophyte screening system...us coordinate and promote the awards. References 1. Martinez-Klimova, Elena et al. “Endophytes as sources...antibiotic” Biochemical Pharmacology 2017 Jun 15;134:1-17. PubMed PMID: 27984002. 2. Bilan, Dmitry S. et ...Addgene-Seeding Labs Plasmid Grant Check Out Previous Addgene-Seeding Labs Plasmid Grant Awardees Listen to Our...Labs and Addgene. The Addgene-Seeding Labs plasmid grant is a collaborative endeavor providing plasmids to...who were awarded the Addgene-Seeding Labs Plasmid grant this year. These scientists work on projects spanning...and to increase the potential for this drought tolerant plant, Dr. Robertson aims to make use of the CRISPR...

  13. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...available as a substrate for another round. Figure 1: Overview of prime editing. Created with BioRender.com...incorporated into the DNA by reverse transcription. The edited DNA strand displaces the unedited 5’ flap and...replace” gene editing method in which a reverse transcriptase (RT) is fused to the C terminus of Cas9 H840A...the target sequence where it nicks the non-target strand and generates a 3’ flap. The 3’ flap binds to the...Moloney murine leukemia virus (M-MLV) reverse transcriptase. Engineering of the PE enzyme and related components...including exploring new sources for the reverse transcriptase. Many PE enzymes and related tools are available...

  14. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...each reporter or check out table 1 for a summary. HRP HRP tagged RANbodies don’t require a secondary antibody... Table 1: Summary of the Key Features of HRP, Spaghetti Monster, and Chicken IgY Domain RANbody Reporters...NIH’s Developmental Studies Hybridoma Bank (DSHB). 1 ml of antibody-containing hybridoma supernatant is... generate and costly to share. What is a RANbody? A RANbody is a fusion between a reporter and a nanobody...nanobody sequence could be used to make a RANbody. RANbodies are expressed in 293T cells and are secreted...smaller size of RANbodies compared to antibodies. Staining with multiple HRP-RANbodies is indistinguishable...HRP-conjugated RANbody can be used at a time. Samples can be sequentially stained with two HRP RANbodies if the...

  15. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...course much more difficult than in cell culture, but Ran et al. have shown that CRISPR and AAV can be used...Thakore PI, Moreb EA, Rivera RM, Madhavan S, Pan X, Ran FA, Yan WX, Asokan A, Zhang F, Duan D, Gersbach CA...WL, Widrick JJ, Yan WX, Maesner C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church GM, ...: 25692716. PubMed Central PMCID: PMC4335351. 9. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz...X-linked recessive disorder affecting approximately 1 in 5000 males. DMD is caused primarily by frameshift... applicable to 80% of DMD patients. Figure 1: Exon skipping strategies for Duchenne muscular dystrophy...needed. First, CRISPR delivery must be optimized to: 1. Reach a high percentage of muscle cells throughout...

  16. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...obstacles when working with virus: 1. Viral vectors undergo DNA rearrangements. Depending on the composition...approximately 20–50% of the packaging cells after 1–3 days of selection. A lower percentage of surviving...up-to-date tips for your virus experiments. References 1. Anthony, Simon J., et al. "A strategy to estimate...may undergo genomic rearrangements during replication. To avoid genomic rearrangements, try amplifying the...immediately prior to their transduction has been shown to increase AAV transduction efficiency by approximately...immediately following transfection. In other words, when exchanging the medium after transfecting cells, replace...Fibronectin is another membrane-interacting protein that can be used to enhance transduction efficiency in cells...

  17. Tips for arabidopsis transformation

    Type
    Blog Post
    ...if you obtained a transformant with a single insertion because it will segregate 3:1 in the T2 generation...lineage and has made countless transgenic plants in the process.     References 1. Emami, S., Yee, M. and Dinneny...Methods. BioMed Central, 2(1), p. 19. doi: 10.1186/1746-4811-2-19. PubMed PMID: 17087829. PubMed Central... which is ease of transformation. There are many motivations to generate transgenic Arabidopsis, from ...from studying transcriptional and translational dynamics of genes and proteins in living plants, to complementing... dip transformation method. The general steps for this method include: Cloning and transforming a plasmid...integrates transfer DNA (tDNA) into the genomes of the plants it attacks Growing the transformed agrobacterium...

  18. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...64325 3xFLAG-dCas9/p-bacteria 3xFLAG-dCas9 pLtetO-1 Fujii Efficient isolation of specific genomic regions...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type... opposite strands of your DNA in close proximity. These double nicks create a double-strand break (DSB...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...base editors convert C->T (or G->A on the opposite strand) within a small editing window specified by the...

  19. Arf GTPase Family

    Type
    Collection
    ...component involved in GTPase signaling activity. Figure 1: Locations of ARF superfamily members. Figure from...regulators of membrane traffic, they also play critical roles at cilia, centrosomes, the plasma membrane, lipid...2016). Tracing the archaeal origins of Eukaryotic Membrane-Trafficking System building blocks. Molecular ...., Dacks, J. B., Lambright, D. G., Lee, F. S., Randazzo, P. A., Santy, L. C., Schürmann, A., Wilhelmi,...

  20. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...focus on the AAV transfer plasmid and take a look at each of its parts. Figure 1: Parts of an AAV...human synapsin 1 promoter is neuronal specific (Haery et al., 2019). Most AAV transfer plasmids have a...repeats (ITRs) are what makes an AAV transfer plasmid an AAV transfer plasmid. ITR sequences are 145 bases...read through this challenging sequence. Transgene The transgene is the gene that will be delivered by the...control transgene expression. Lox sites are directional 34 bp sequences that can flank the transgene and ...(WHP) Posttranscriptional Regulatory Element (WPRE) forms a tertiary structure when transcribed which ... nuclear export of RNA and RNA translation, and promotes RNA transcript longevity. The polyA is located...
Showing: 61 - 80 of 736 results