We narrowed to 612 results for: RET

...qualitative. The Organized Smooth Endoplasmic Reticulum (OSER) assay (Costantini et al., 2012) measures...homodimerization of FPs targeted to the endoplasmid reticulum based on the appearance of OSER whorls. This assay...considered a linker (figure 1). In fact, for several FRET biosensors (yellow cameleon 3.60, EPAC, and Galphai...fluorescent protein can be truncated to increase the FRET efficiency. Creating fusions at the N- and C- termini...technology and should always be considered when interpreting results.  The recent revolution in genome editing...Klarenbeek, Jeffrey, et al. "Fourth-generation epac-based FRET sensors for cAMP feature exceptional brightness,...PubMed PMID: 30886412. Mastop, Marieke, et al. "A FRET-based biosensor for measuring Gα13 activation in...

...interaction that increases FRET transfer between the fluorescent proteins. FRET is highly amenable to biosensor...biosensor creation, and custom FRET biosensors can be created using the cpFRET kit created by Olivier Pertz... increases in fluorescence of up to 11,000-fold. FRET biosensors are another common class of biosensors... Tsien’s lab contain two fluorescent proteins (a FRET pair) separated by CaM and M13. When calcium binds...such as the nucleus, mitochondria, endoplasmic reticulum (ER), vacuole or cellular membranes. Examples ...

...Cameleon-based FRET sensor D1ER, and GCaMPER. Bonus: There are also Cameleon-based FRET sensors available...CEPIA) or through FRET sensors, can reduce these unwanted variables. Twitch is a FRET-based calcium reporter...fundamental to muscle and nerve function, hormone secretion, blood clotting, and as we all know, a structural...available biosensors use a single GFP or CFP/YFP-derived FRET pair. If you need to free up crowded optical space...buffering and to increase sensitivity), and is a great FRET sensor option if you have the setup necessary for...

...mScarlet's emission via FRET.  Image from Beck et al, 2019 under CC BY 4.0.   FRET Chemigenetic Sensors...FP-fusion to rhodopsin resulted in electrochromic FRET capable GEVIs, with rhodopsin quenching the added...and available with red-shifted fluorophores (find FRET capable GEVIs here!). The only issue with these ...variety of different components, but all contain a FRET pair, a genetic component, and a small molecule ...modulate the degree of quenching of the fluor via FRET. This system is particularly powerful for in vivo...Optogenetics Fluorescent Proteins 101: Introduction to FRET ...

...plasmid addiction systems” require dividing cells to retain plasmids - if a cell does not inherit plasmid due...the more stable toxin. Cells only survive if they retain the plasmid. Since their discovery, plasmid addiction...by the gene of interest. As a result, cells that retain the ccdB gene die off and a higher percentage of...systems onto plasmids allowed cell populations to retain more of these plasmids as compared to those without...after 24 hours, the unstabilized plasmids were retained in less than 10% of cells while plasmids containing...containing the plasmid addiction system were retained in 75% of cells. They were able to apply this system...adapted by researchers for many uses when plasmid retention without selective pressure is necessary or for...

...Desfarges & Ciuffi, 2012). One family of viruses, retroviridae, require integration for their life cycle to... during production (read our blog post on the retroviral life cycle for a more detailed description). ...concerning in clinical settings, as evidenced by retroviral gene therapy trials for X-linked severe combined...remember: You can never trust a lentivirus with secrets... Because they tend to insert themselves into ...immunodeficiency virus type 1 integrase mutants retain in vitro integrase activity yet fail to integrate...lessons from provirus and non-integrated DNA. Retrovirology, 1, 13. https://doi.org/10.1186/1742-4690-1-...resources on the Addgene blog Viral Vectors 101: The Retroviral Life Cycle Quick Guide to All Things Lentivirus...

...Christian Gram realized that certain stains were retained only in some species of bacteria. The stain colored...classification system. Eventually, any bacteria that retained the stain became known as gram positive, and the...and the ion interactions that cause the stain retention in gram positive bacteria was discovered in 1983...bacteria’s thinner membrane. Procedure The stain is a pretty simple process. First, flood the heat-fixed smear... thin inner peptidoglycan membrane is unable to retain the stain, leading to its decolorization. In contrast...stained by the crystal violet dye, but is easily retained in the decolorized gram negative bacteria, staining...

...excitation profiles is vital for the accurate interpretation of FRET-based experiments. A popular combination... elevation in this excited state, the electron returns to its ground state by emitting a photon with slightly...Salari et al., 2019). Photostability, the ability to retain fluorescent intensity during exposure to light,...labeled agonists and antagonists, impacting the interpretation of experimental results. It's important to ...using fluorescently tagged ligands or intermolecular FRET for ligand-receptor interactions, require specific...tagging. In Fluorescence Resonance Energy Transfer (FRET) applications, the dyes need to have compatible ...

...difluoride (PVDF) membrane, which is sturdier and retains the blotted protein better than a nitrocellulose...transfer, dry the membrane to maximize protein retention. Following this step, most PVDF membranes recommend...proceed with caution. Unlike PVDF, they can be returned directly to buffer without a re-wetting step. ...plan to reprobe! Just as your sample protein is retained better, antibodies will stick permanently to a...membrane once before any detection steps to avoid retaining residual antibodies. If you have already applied...membrane Alright, so you’ve dried your membrane and returned it to buffer, you’ve run and imaged your western...

... ShadowR, a novel FLIM-FRET acceptor Figure 4: ShadowR is used as a FRET acceptor to mRuby2 or mScarlet...microscopy-based Förster resonance energy transfer (FLIM-FRET) in living cells (Figure 4). They created ShadowR...inhibit their activity. ShadowR can be used as a FRET acceptor in combination with mRuby2 or mScarlet ...Fluorescent Protein 101 blog series Learn more about FRET Find protocols and tips for making plasmid constructs...our Molecular Biology Reference Find plasmids for FRET References Blanquet RS, Phelan MA (1987) An unusual...

...generated a series of retroviral vectors expressing tagged dCas9 and guide RNA. The retroviral enChIP system ...necessary to isolate specific genomic regions while retaining molecular interactions. For this purpose, we developed...requested plasmids for enChIP through Addgene. Retroviral Expression System for enChIP using CRISPR Addgene...Addgene: Your lab recently developed a retroviral enChIP system using CRISPR technology. Can you describe...Associated with an IFNgamma-Responsive Promoter by a Retroviral Expression System for enChIP Using CRISPR. Fujita...

...based on our most accurate interpretation of the world, and this interpretation should not effected by claims... government and, consequently, politics. More concretely, scientists gather information about reality ...however, inconvenient data must not be ignored. Returning to an earlier analogy, no amount of shouting will... of depending on scientists or politicians to interpret it for them. Other popular reasons to march include... not in the job of having evidence’ on Trump’s wiretap claim The Chronicle of Higher Education: “March...

...positive and negative controls to ensure proper interpretation of results. A positive control is generally.... This information is critical for correctly interpreting experimental results.  Replicates Replicates... needs to be addressed. In order to correctly interpret your results, the signal from your target needs...autofluorescence, also interferes with data interpretation and can be addressed with a label control. ...autofluorescence and should be considered in the interpretation of results. Include the label control for all...membrane. A loading control is critical for data interpretation, especially when changes in protein expression...

...include the quality control of secretory proteins in the Endoplasmic Reticulum, optimization of fluorescent...committee members provide expertise in designing and interpreting experiments, as well as career guidance. In ... controls? Will these controls enable you to interpret your results? Will the experiment actually answer...mentor, manage, communicate, and teach The dirty secret about becoming a group leader is that people are...student. Learn to teach how to design, perform, and interpret experiments. You'll be better prepared for your...

...information, accession numbers, sequences and lineages, FRET efficiencies, additional references, etc...) and...frequently) for the sake of comparison or ease of retrieval.  These collection pages can be kept private, ...be downloaded in JSON or CSV format to quickly retrieve all of the information in the database relating... applications, a REST API allows programmatic retrieval of the majority of information in the database...all fluorescent protein plasmids Find plasmids for FRET Check out the Michael Davidson Fluorescent Protein...

...energy transfer (FRET)-based executioner caspase reporters. In these reporters, the FRET pair is linked ...emission. FRET and fluorescence based reporters however have several limitations. First, FRET signals are...a clear pattern in the developing forebrain and retina. This pattern was consistent with TUNEL staining...

...microvesicles (approx. 40 - 150 nm diameter) that are secreted from the cellular membrane into the culture medium...demonstrated in different cellular environments such as retina or glioblastoma). They also found that exo-AAVs...AAVs (Schiller et al., 2018). Exosomal vesicles secreted from producer cells also contain cellular macromolecules...deafness (György et al., 2017), exo-AAV2 in mouse retina after intravitreal injection to transduce bipolar...vector mediates robust gene delivery into the murine retina upon intravitreal injection." Scientific reports...

...plasmid deposit please sign up here.        A retroviral mouse CRISPR knockout library Article contributed...issue, Sarah Teichmann and colleagues created a retroviral CRISPR knockout library using gRNAs designed ...designed by the Kosuke Yusa Lab. This retroviral library was used to transduce T helper type 2 (Th2) cells from...differentiation factors as the screen readout. This retroviral mouse CRISPR pooled library can be used for screening...These include Arch(D95N) and QuasAr, as well as the eFRET sensors, QuasAr-mOrange and MacQ-mCitrine. All of...

..., the solution is diluted, complicating the interpretation. Because of this, these methods only give a...direct the fluorescent protein to the Endoplasmic Reticulum (ER). If homodimerization occurs (most likely ...structures known as Organized Smooth Endoplasmic Reticulum (OSER) whorls are visible. These whorls can be...CytERM-mTurquoise2, and CytERM-dTomato. When using and interpreting the results of the OSER assay, you should keep...Amsterdam) where she develops genetically encoded FRET based biosensors.     Additional Resources on the...

...“no.” When there was room for misinterpretation, that misinterpretation was definitely happening. Our ...were leaving these meetings with different interpretations of what we discussed and decided. I needed ...Whether or not the container was barcoded (this is pretty Addgene specific) The types or purposes of each...that seemed simple, but they could in fact be interpreted in several different ways depending on the team...