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Showing: 61 - 80 of 582 results

  1. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Structure Plasmids Phamret 458 517 14 Monomer Phamret-N1 - Mammalian Expression Phamret-C1 - Mammalian Expression...Empty Backbones More Fluorescent Protein Resources: FRET Biosensors Subcellular localization Michael Davidson...- Mammalian Expression pMXs-mRFP1 - Mammalian Retroviral Expression mCherry 587 610 16 4.5 15 min Monomer...

  2. CRISPR Guide

    Type
    Collection
    ...coding sequence ~1 kb shorter than SpCas9 while retaining the same basic function. Its size allows it to...AAV backbones for in vivo experiments and in a retroviral backbone for delivery to cells that are poorly...Cas9 Fusion Tools dCas9 cannot cleave DNA, but it retains the ability to find specific DNA targets through...specificity, and editors carrying an ADAR truncation that retain RNA editing capabilities but are small enough to...23979020 Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W.,...., Terns, M. P., White, M. F., Yakunin, A. F., Garrett, R. A., Van Der Oost, J., . . . Koonin, E. V. (...: 32572269 Charpentier, M., Khedher, A. H. Y., Menoret, S., Brion, A., Lamribet, K., Dardillac, E., Boix...

  3. Tetracycline Inducible Expression

    Type
    Collection
    ...plasmids third-generation rtTA On Elledge 19407 pTREtight2 Promoter contains a modified TRE that is silent...Expresses firefly luciferase hairpin and GFP under pTREtight promoter None Either Elledge 35625 pAAV-Ptet-RFP-shR-rtTA... 3rd generation rtTA On Campeau 25434 pMA2640 Retroviral; CMV-driven; linked via IRES to EGFP-Blasticidin...EGFP-Blasticidin fusion; pMA2641 has rtTA driven by retroviral LTR promoters ; see article for lentiviral vectors...

  4. Optogenetics AAV Preps

    Type
    Collection
    ... Optogenetics AAV *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? ...viruses would be helpful to the scientific community. Return to top...

  6. mTOR Pathway

    Type
    Collection
    ...was generated with the help of David Sabatini . Return to top mTOR Pathway - Gene List Click on a name...sclerosis VHL Von Hippel-Lindau tumor suppressor Return to top mTORC2 Complex Symbol Name AKT AKT1 AKT2...complex 2 SGK Serum/glucocorticoid regulated kinase 1 Return to top Resources Cancer Pathway ORF Kit (Sabatini...

  7. AAVED

    Type
    Collection
    ... AM Cre and Flpo Katherine Matho 10:15 AM AAV Retrograde Tracing Gowan Tervo and Sarada Viswanathan 11...of research materials to share: In vivo data: Retrograde hSyn1-GCaMP6f-P2A-nls-dTomato Detailed protocol....doi: 10.3389/fnana.2019.00093. PubMed 31849618 Return to top...

  9. Worm Expression Resources

    Type
    Collection
    ..., or by suggesting plasmids for the collection. Return to top Plasmid Kits and Collections Kits are sets...synthetic biology plasmids for use in C. elegans . Return to top Other Resources Addgene Blog Posts An “elegans...genome of the genetic model organism C. elegans. Return to top Do you have suggestions for other plasmids...

  10. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...AICSDP-41 mEGFP SERCA2 Sarcoplasmic reticulum/endoplasmic reticulum 114406 SMC1A-mEGFP AICSDP-57 mEGFP ...SEC61B-mEGFP AICSDP-7 mEGFP Sec61 beta Endoplasmic reticulum 87427 FBL-mEGFP AICSDP-13 mEGFP Fibrillarin Nucleolus...

  11. Ras Pathway

    Type
    Collection
    ...Frederick National Laboratory for Cancer Research Return to top Ras Pathway - Gene List Click on a name ...RASSF10 Ras association domain family member RB1 Retinoblastoma 1 RCE1 Ras converting CAAX endopeptidase 1 ...exchange factor 1 YAP1 Yes associated protein 1 Return to top Resources RAS Pathway Clone Collection 2.0...

  12. Lentivirus Plasmids

    Type
    Collection
    ... Transfer Plasmids Lentiviruses, a subset of retroviruses, are some of the most common and useful types...MSCs. Use packaging construct encoding Tat. Reiser Return to Top Popular Lentiviral Transfer Plasmids The...article for more versions of this plasmid. Root Return to Top Do you have suggestions for other plasmids...

  13. Neurodegeneration Research Collection

    Type
    Collection
    ...major burden on the patients, their families and caretakers, and the health system in general. The required...Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian.... elegans. Yemini et al. Cell. 2021 Jan 7. Use a FRET-based method to monitor structural transitions in...

  14. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGTTAAGGCTCTGTATAGcagtgtgtgtcctggcCACTTGCTGGGCTCAGGA retinitis pigmentosa 2 (X-linked recessive) TAL3526 & TAL3527...TCTTTTTGTGCTGCGCCAcccagcccataatcgaGTCTTCTACAGGCATGAA retinitis pigmentosa GTPase regulator b TAL3528 & TAL3529...TGGAGAAACAGAAGATGAaatccccgagtccggaGCTGTCTTCACTTTTGGA retinitis pigmentosa GTPase regulator b ORF15 TAL3530 & ...

  15. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...20 bp in length and paste them into contiguous stretches of DNA known as CRISPR arrays. Separate Cas proteins.... PMID: 28931002 Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini ... FJ, Terns RM, Terns MP, White MF, Yakunin AF, Garrett RA, van der Oost J, Backofen R, Koonin EV. 2015...

  16. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...powerful the FRET signal. In well-designed FRET-based biosensors, the intensity of the FRET signal can ...packaged into a retrovirus for infection of host cells. The fluorescent moiety of the BRET reporter was ...proteins together thereby increasing their FRET signal. FRET is a non-radiative transfer of energy from...accuators. The researchers took a FRET reporter and adapted it into a BRET reporter by using NanoLuc® Luciferase... allows researchers to convert FRET biosensors into more versatile BRET biosensors that can even be paired...PMC5171807. 6. Machleidt, T., et al. (2015) NanoBRET-A novel BRET platform for the analysis of protein-protein... on the Addgene Blog Learn more about FRET Tips for Using FRET in Your Experiments Learn more about Fluorescent...

  17. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...intracerebroventricular (ICV) delivery in newborns, and retro-orbital (RO) and stereotaxic (STX) delivery in adult...pAAV-AiE2435m-minBG-SYFP2-WPRE3-BGHpA AiP1848 AiE2435m SYFP2 Cajal-Retzius neurons Hippocampal formation 214597 pAAV-AiE2563m-minBG-SYFP2...Oyama A, Pham T, Pom CA, Potekhina L, Ransford S, Rette D, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sedeno-Cortes...

  18. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...developed a new AAV variant, rAAV2-retro, that migrates retrogradely at higher rates than any other AAV... AAV2-retro vectors with a variety of transgene/promoter combinations. Check out our retrograde AAV blog...Selective retrograde transsynaptic transfer of a protein, tetanus toxin, subsequent to its retrograde axonal...strengthened during memory formation and memory retrieval. In neuronal tracing experiments, brain tissue...bodies to the terminals of their axons; while retrograde tracing outlines neurons in the opposite direction...axons to their cell bodies. Anterograde and retrograde tracing take advantage of existing transport ...myosin, and enzymes for transmitter synthesis. Retrograde transport is used for transport of endocytosed...

  19. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...viruses: gamma-retrovirus, lentivirus, adenovirus, and adeno-associated virus. Gamma-retrovirus Retroviruses...type of gamma-retrovirus is Murine Leukemia virus (MLV). Naturally isolated gamma-retroviruses have been ...Lentivirus is a type of retrovirus and shares many common features with gamma-retrovirus including genome architecture... nucleus as gamma-retroviruses do. Fun fact: Since lentivirus and gamma-retrovirus are so closely related...expression (Robinson, 1982). To add insult to injury, retroviruses generally have moderate to high immunogenicity...immune response in the host. So why use a gamma-retrovirus in the lab? Well, they are essentially evolutionarily... types with a high efficiency. However, gamma-retrovirus infection is restricted to dividing cells only...

  20. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...compose the secretory pathway, endocytic vesicles, mitochondria, lysosomes or they are secreted into the ...blocked from proceeding through the secretory pathway, and retained in the ER. FPs have been undeniably...include the quality control of secretory proteins in the Endoplasmic Reticulum and live cell imaging approaches... the power of the Dark Side." --Darth Vader in "Return of the Jedi" While Vader was referring to the evil...what if an FP fusion with a resident endoplasmic reticulum (ER) protein instead localizes to the cytoplasm...position requirements. For example, a KDEL ER retrieval sequence only functions at the extreme C-terminus...residues form inappropriate disulfide bonds in the secretory pathway (Figure 1) [4,6–8]. Additionally, FPs ...
Showing: 61 - 80 of 582 results