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...jwoodgett.     More Discussions About Data Sharing: PLOS Data Sharing Policy Discussion PLOS’ New Data Policy...this year when it announced its data access and sharing policy. Since early March, the open access publisher...reasonable. PLOS has since clarified their data sharing policy and recently announced that of the 16,000...funding agencies (e.g. Wellcome Trust, NIH Data Sharing Policies, etc.) and is founded on the principle...data should be included? We should recognize that storing data in a sharable form is indeed a significant...Publishers have also contributed to this issue by requiring ever more condensed methods descriptions, legends...existing, published data. The importance of data sharing The reason this topic is important to my lab is...

...interactions, such as exploring viral biology without disturbing the virus of interest and monitoring endogenous ... Plasmid Collection Promega Plasmid Collection Ordering & Availability All Plasmids Plasmid Technology...tags such as NanoLuc, HaloTag, SmBiT, and LgBiT. Ordering & Availability Plasmids from the Promega collection... business days. For more information about the ordering process, check out our help center . Plasmid Collection...sensitivity. These assays are ideal for real-time monitoring of signaling pathways, investigating RNA regulation...

... developed during the COVID-19 pandemic. Our mission to accelerate research through sharing reagents has...2021.03.10.434840 Tsang J, LaManna CM (2020) Open Sharing During COVID-19: CRISPR-Based Detection Tools. The...detect SARS-CoV-2 nucleic acids. Mammalian targets During the 2003 SARS outbreak, Hyeryun Choe’s lab identified...-CoV-2 entry into human lung epithelial cells (Biering et al., 2021). In their search, they found mucins...inhibitors of SARS-CoV-2 entry and become upregulated during viral infection.  Expression of SARS-CoV-2 viral...academic and industry scientists through plasmid sharing and more. As you’ve read above, some of these plasmids....     References and resources References Biering SB,  Sarnik SA, Wang E, Zengel JR, Sathyan V, Nguyenla...

... take a moment to review what exactly you are measuring and how to interpret your results.  Quick note...Purification reagent contaminants The reagents used during nucleic acid purification can leave behind a variety... more considerations and other approaches for measuring DNA quantity and quality than we have covered ...Resources on Addgene.org DNA Quantification Protocol Recovering Plasmid DNA from Bacterial Culture Protocol ...

... learning. 1. Create a peer mentoring group We don’t all get the mentoring support we need, and most of...Own Peer Mentoring Group: A How-To Guide for Scientists.   Once in-person, peer-mentoring groups ...use more mentors to listen, guide and support us during training and throughout our careers. An easy way...goals.  I’ve been surprised to see that virtual mentoring groups seem to be as successful as in person groups...? You could do a talk virtually for your peer mentoring group (see above). However, now that we are all...present, I’m asked what Addgene “looks for” when hiring a scientist. Of course, we have different criteria...

...interference, these enzymes can knock down RNA without altering the genome, but Cas13s have higher on-target specificity...enzymes are the latest tools in the transcriptome engineering toolbox. How were Cas13d enzymes discovered?...MAPT, the gene encoding dementia-associated tau, delivering dCasRx and a 3-spacer array targeting the MAPT... tell how large the genome and transcriptome engineering toolbox will be. It is, however, certain that...that the impact of CRISPR scientific sharing will continue to grow, and we at Addgene appreciate our depositors...References Konermann, Silvana, et al. “Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors...  Mary Gearing...

...Collection under the insect expression system or by filtering by fly as species of gene. Find Gal4 and UAS plasmids...select for transformed bacteria, but you might be wondering, “How do I know which of my flies have the desired...about Using Cre in Drosophila Read Other Genome Engineering Posts Use FLEx Vectors for Site-Specific Recombination... genome engineering...

...and its use as a genetic engineering tool is frequently called recombineering - short for homologous recombination-mediated...components of the Lambda Red recombineering system The lambda red recombineering system has three components...for generating dsDNA recombineering substrates to avoid errors introduced during PCR. ssDNA Substrate ...substrates can be generated by ordering synthetic oligonucleotides or denaturing short PCR products. Either..., Court DL. Recombineering: A Homologous Recombination-Based Method of Genetic Engineering. Nature protocols...Costantino, N. and Court, D. L. 2014. Recombineering: Genetic Engineering in Bacteria Using Homologous Recombination...method that can be used for cloning or genome engineering and is based on homologous recombination. It ...

...by Benoit Giquel. CRISPR has taken the genome engineering world by storm owing to its ease of use and utility...Waltz, 2016), the potential of CRISPR for genome engineering in plants is also being realized. There are a...approaches, these techniques are cumbersome, often requiring several rounds of selection to isolate plants ...plants with the phenotype of interest. Genome engineering, on the other hand, allows for targeted modification...will present a general overview of plant genome engineering using CRISPR, highlight the specific modifications...plants, and outline the various plant genome engineering tools that are available to academic researchers... Addgene. CRISPR components for plant genome engineering CRISPR can be used to knockout, activate or repress...

...important role in scientific exchange. In addition to ensuring affordable access to materials, repositories provide...since 1996.       More Blog Posts about Scientific Sharing: The 10 Most Distributed Plasmid Technologies ...10 Years Data Freedom: The Expansion of Data Sharing in Research Publications ...

...Plamids for Oligomerized Pool ENgineering (OPEN) method has been used to engineer ZFNs that function ...used to engineer ZFNs via the Oligomerized Pool ENgineering (OPEN) method. This kit consists of 5 individually...of Kit Original Publication Oligomerized pool engineering (OPEN): an 'open-source' protocol for making ...Joung et al., PNAS 2000 ). The Oligomerized Pool ENgineering (OPEN) method has been used to engineer ZFNs ...For your Reference section: Oligomerized pool engineering (OPEN): an 'open-source' protocol for making ...

...SynBio Assembly Standards SynBio Blog Genome Engineering SynBio Depositing Labs Hal Alper J. Chris Anderson...Weiss Synthetic biology describes the rational engineering of biological systems for novel functions. Addgene... biologists around the world to facilitate the sharing of parts and information. Addgene can help you ...Regulation Browse plasmids containing both naturally occurring and synthetic regulatory elements, including promoters...Bio-Energy Institute Synberc - Synthetic Biology Engineering Research Center The PLOS Synthetic Biology Collection...

...light orange, that bind to a specific antigen, triggering an immune response. The stem of the Y structure...valency of 2 IgG1; IgG2; IgG3; IgG4 IgM Produced during the primary immune response; found mostly in blood...protein:protein complex. Due to their increased manufacturing requirements, monoclonal antibodies are typically...tolerate freeze-thaw cycles. Here are a few tips for storing your antibody: Antibodies conjugated to fluorophores...a similar assay type) and search again. After filtering, narrow down further by company, price, and other...assay with the results of a non-antibody assay measuring either the same output or a proxy for that output... antibody validation This method validates by comparing your antibody of interest to a second, independent...

...contributions from depositors around the globe, we’re now sharing over 100,000 reagents including plasmids, ready-to-use...

... to academic scientists. Expanding plasmid sharing during COVID-19 Realizing this limitation, we worked...members of the scientific community support open sharing. But, one critical part was missing when the COVID...setting.  In light of the need for basic research during the current pandemic, universities are showing ...cells. We teamed up with Ginkgo Bioworks to quickly bring these plasmids to the scientific community - both...available to industry scientists To make plasmid sharing more open for academic and industry scientists,...the past months, we’re excited to see our open sharing impact grow beyond COVID-19. But, we can’t do this...office to discuss the possibilities for expanded sharing. Is there a plasmid you're hoping will be come ...

...career path and why he chose to go into genome engineering. Chapter 1: Early research on CRISPR and what...with new project ideas, applying for grants, and mentoring. Chapter 5: Wei Leong Chew's lab management style... 6: Projects in Wei Leong Chew's lab. Genome engineering, gene delivery methods, AAVs, and more. Chapter... genome engineering...

...raise salaries prior to the injunction are still honoring these plans. We are now collecting data on the...who had a contract - negotiated by their union - securing higher salaries. This decision will carry over...geographical regions When we originally started gathering this information, many assumed that those living...Read Gary's Previous Article: Changing Labor Laws Bring Increased Postdoc Wages Read about the Advocating...

...these extreme environments. Gearing up for sample collection at the hot springs My husband, Paul Blainey,...all of the remote areas, including one hot spring requiring an overnight hike in. Navigating challenges...the hot spring. Hiking all day and processing samples by night was fun but also very tiring. It feels...Lab at Stanford University became interested in exploring biological dark matter – large tracts of the microbial... these microbes. From 16S rRNA sequencing, hot springs were known to be diversity hotspots containing ...aquaticus bacterium – was discovered at Octopus Hot Spring at YNP, and since then, many researchers have searched...temperatures and unstable ground around these hot springs make them extremely dangerous, we used telescoping...

...large scale cell mobility are essential processes during embryonic development. These are required for a...development of cell sorters together with the engineering of fluorescently labelled antibodies made it ...)? How do blood monocytes patrol blood vessels during immune responses (Auffray et al. 2007, Finsterbusch...relatively little tissue absorption, weak tissue scattering and small amounts of tissue auto-fluorescence...understanding the key roles of immune cells in triggering an immune response. New fluorescent proteins ...Dynamics of neutrophil migration in lymph nodes during infection." Immunity 29.3 (2008): 487-496. PubMed...Jackson G., et al. "Macrophage and T cell dynamics during the development and disintegration of mycobacterial...