We narrowed to 91 results for: SHR

1. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

   Type

   Blog Post

   ...identified essential genes while previous Cas9 and shRNA library screens only identified  60% of the essential...

2. Deciphering the Mysteries of Behavior with Viral Vectors

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   Blog Post

   ...paper, the authors used lentivirus to introduce an shRNA to downregulate expression of miR124a, a regulatory...

3. Neurodegeneration Plasmid Collection

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   Collection

   ...Parkinson's David Root 78523 pFUChW Parkin shRNA Mul1 shRNA PRKN H1 Parkinson's David Chan 78622 pX335...Josef Kittler 163858 VPS35 shRNA1 VPS35 Parkinson's Lei Lu 163859 VPS35 shRNA2 VPS35 Parkinson's Lei Lu ...pAAV-CMV-GFP-pA U6 shRNA (Rat Nurr1 #1) NR4A2 GFP CMV Parkinson's Glenn Schafe 233274 pAAV-CMV-GFP-pA U6 shRNA (Rat ...ALS George Church 222870 pLKO.1 mTagBFP2 rat DJ-1 shRNA PARK7 U6 Parkinson's Timothy Ryan 223563 pmCherry-Synaptojanin...

4. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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   Blog Post

   ...., 2008). 2. Transient expression of transgenes/shRNAs If you want your cells to express a protein or ...

5. CRISPR 101: Homology Directed Repair

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   Blog Post

   ...DNA double-strand break repair pathway choice. Shrivastav M, et al Cell Research (2008). PubMed. Mechanisms...

6. A Control for All Seasons

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   Blog Post

   ...substituting with knockdown approaches using siRNA or shRNA. If this alternative is not possible due to a lack...

7. Plasmids 101: Restriction Cloning

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   Blog Post

   ...choose. CIP (calf alkaline phosphatase) or SAP (shrimp alkaline phosphatase) are commonly used. Follow...

8. Building Global Connections with the International Mentorship Program USA-EUROPE

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   Blog Post

   ...always significant. Doubts, fears, insecurities… all shrink when shared with a mentor who has experienced them...

9. Antibodies 101: Antibody Engineering and Directed Evolution

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   Blog Post

   ...evolve. With this added layer of insight, you can shrink your mutant library and increase the chances of...

10. Important Considerations When Using AAVs

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    Blog Post

    ...such as microRNAs (miRNAs), short hairpin RNAs (shRNAs), antisense oligonucleotides (ASOs), zinc finger...

11. Quick Guide to All Things Lentivirus

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    Blog Post

    ...lentiviral vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors...

12. Immunology Research Plasmids and Resources

    Type

    Collection

    ...hormone, beta polypeptide - FSHR follicle stimulating hormone receptor FSHRO, LGR1, MGC141667, MGC141668...CHNG4, TSH-BETA TSHR thyroid stimulating hormone receptor CHNG1, LGR3, MGC75129, hTSHR-I TSLP thymic stromal...

13. Plasmid Collections

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    Collection

    ...all pooled libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor Collections A modular set...

14. CRISPR Plasmids - Cascade-Cas3

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    Collection

    ...target region, effectively turning Cas3 into a DNA “shredder” system that can cause large genomic deletions...

15. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

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    Protocol

    ...MCS) Adding short tags - ex: HA or Flag Cloning shRNAs Background For the purposes of this tutorial we...can be used just as easily to add short tags or shRNAs to any vector (the procedure will simply differ...

16. Luciferase Plasmid Collection

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    Collection

    ...promoters, enhancers and untranslated regions, or shRNA or CRISPR target sites can be inserted into a luciferase-containing...

17. Plasmids for Stem Cell Research

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    Collection

    ... of human Sox2, KLF4, L-Myc, Lin28, OCT3/4, and shRNA against p53 in different gene/insert combinations...

18. 27 Hot Plasmids from 2016

    Type

    Blog Post

    ...expressing GFP, tetracycline response elements, and shRNAs many of which are available at Addgene. Since GMAP...

19. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type

    Protocol

    ...insert. CIP (calf alkaline phosphatase) or SAP (shrimp alkaline phosphatase) are commonly used. Follow...

20. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type

    Protocol

    ...choose. CIP (calf alkaline phosphatase) or SAP (shrimp alkaline phosphatase) are commonly used. Follow...