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  1. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to be integrated can be cloned into the vector...weeks, depending on the virus, cell type, and/or desired event.   Figure 1: Transduction to expression...

  2. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ... my curiosity did not ultimately translate to a desire to do the research itself. Several years of exclusively...more creative faculties into motion, such that a desire to once again write for fun eventually saw an English...

  3. RUBY-Red Siliques

    Type
    Blog Post
    ... Antibiotic selection would only then kill the desired plants! PCR or fluorescent markers are currently...from these plants will be green, containing the desired mutation but lacking both RUBY and the construct...

  5. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...if you end up concentrating your AAV above your desired titer and want to dilute it, err on the side of...less buffer than you would to achieve the exact desired concentration to avoid over-diluting, and make ...

  6. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...the plasmid, which is then introduced into the desired mammalian, plant or bacterial cells. Expression...stop codon, which is then transfected into the desired cells. A hybrid mRNA transcript containing luciferase...

  7. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...photobleach at some point. For live-cell imaging, it is desirable to have fluorescent proteins that are photostable...photoswitching (Kremers et al, 2009), which usually are undesired properties. In the ideal situation, a fluorescent...

  8. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...of ccdB, leading to positive identification of desired clones.   ...Becomes a powerful cloning tool... efficiently, as those that do not contain the desired insert should not grow.   Figure 2: Image...

  9. All in a Twist: dsRNA

    Type
    Blog Post
    ...all the diverse in vitro production mechanisms. siRNA, also known as RNAi, are short, dsRNA molecules ...therapeutic delivery. Thus, balancing the amount of siRNA to be delivered or anticipating this response are...

  10. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...that mismatch repair (MMR) interfered with the desired resolution of prime editing intermediates. Building...sequence. As with other prime editing tools, the desired edits are templated by the 3′ extension of the ...

  11. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...is tested following each immunization until the desired level of antibodies is reached - typically within...within 2-4 months. After the animal has the desired level of antibodies in its bloodstream, the antibodies...

  13. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...transcription of the template and initiating the desired cleavage event, although some engineered terminators...downstream elements. A high affinity terminator may be desired for multi-cistronic constructs where high termination...

  16. Plasmids 101: Protein Expression

    Type
    Blog Post
    ... Inducible promoters are extremely useful if a desired protein product is toxic to cells at high concentrations...allows for easier access and purification of a desired protein. Despite being a rather nascent technology...

  17. Pooled Library Amplifications

    Type
    Blog Post
    ...(Are the inserts of different sizes?), and the desired coverage for the library (How important is it that...  The most important factor to consider is your desired library coverage, since this will help decide what...

  18. PhD Applications After COVID

    Type
    Blog Post
    ...to introduce yourself to a few members of your desired department during the application process. Many...However, an online meeting with members of your desired program will allow for you to have the time and...

  20. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...primary method to produce the linear fragments, as desired modifications and homologous regions can be encoded...order primers that bind at either side of the undesired portion, designed to amplify the rest of the vector...
Showing: 61 - 80 of 227 results