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We narrowed to 238 results for: SPL

Showing: 61 - 80 of 238 results

  2. Hot Plasmids: Summer 2025

    Type
    Blog Post
    .../doi.org/10.1038/s41477-025-01989-9.   Make a splash with new LARRY barcoding libraries By Mike Lacy... is reported to target skeletal muscle, kidney, spleen, lung, heart, stomach, and brain. C) Recent studies...FingR = fibronectin intrabody generated with mRNA display. Modified from Rivera & Huang, et al. (2025) under...

  3. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...talking to themselves as they compare the features displayed on the model with their mental model of the protein...primed for cleavage. This structure captures the displaced non-target DNA strand and shows the HNH nuclease...target DNA strand. In addition, this model also displays the several Lys/Ala mutations reported by Ian ...

  4. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...sequence NGG compared to wild-type SpCas9, which also displays some cleavage with an NGA PAM. This variant may... AAV delivery. NmCas9, another small ortholog, displays lower off-target editing than wild-type SpCas9...rather than the blunt ends created by Cas9. Cas12a  displays lower off-target editing than SpCas9 (Kim et al...

  5. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...each sample. Successfully analyzed samples will display the following parameters: Sample - The unique ...analysis can be sorted by any of the parameters displayed in the summary table. You can also use your browser..., you’ll find an Indel plot (left panel) which displays the inferred distribution of indel sizes in the...

  6. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...developed into a split aptamer that can be used to investigate RNA assembly. The split aptamer is composed...References Alam, Khalid K., et al. "A fluorescent split aptamer for visualizing RNA–RNA assembly in vivo...

  7. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...for DNA repair synthesis. The ssDNA strand then displaces one strand of the homologous DNA donor and can...formation of a DNA structure referred to as the displacement loop (D loop). The recombination intermediates...synthesized portion of the invasive strand is displaced from the template and returned to the processed...

  8. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...abundant and ethanol concentrations are low, pADH1 displays low promoter activity (~20% activity of the strong...complexity of the system, researchers have used split GAL4 architecture or combined the system with Flp...Valle Rodriguez, Alberto, Dominic Didiano & Claude Desplan. “Power tools for gene expression and clonal analysis...

  9. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...negative effect on these parameters. IT5 and IT6 displayed good binding efficiency in both immunofluorescence...cyclin Cdc28 and the chaperone Ydj1, both of which display sophisticated interactions with multiple other ...

  10. Science as Art

    Type
    Blog Post
    ...Jurassic Technology in LA, where it is currently on display.   Many universities are now following the trend...scientific art competitions where creations can go on display for all to enjoy. The largest competition of this...

  11. Pipetting Protocol

    Type
    Protocol
    ... expel liquid into the pipette tip, the volume display which shows the set volume for the the pipette,...section will help you understand how to read each display to dispense the correct amount of liquid reagent...), while 0.5 µL would read as 050. Each volume display on these pipettes will also have small tick marks...you will be rotating dials behind the numerical display or the top of the plunger of the pipette. Select...

  13. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...small (Rep52 and Rep40) Rep proteins. Alternative splicing generates the different C-terminal regions of ...often driven by the AAV p40 promoter. Alternative splicing and differing translational start sites generate...

  15. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...iATPSnFR1.0 and iATPSnFR1.1 (cytoplasmic, membrane-displayed, expression in neurons, astrocytes) A genetically... 1720648115. Katalin Torok Glutamate Membrane displayed variants of fluorescent glutatmate sensors (iGluSnFR1...

  16. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...movement. When the ribosome moves along an mRNA, it displaces other RNA-binding proteins to allow translation...translation to occur. For a TRICK reporter mRNA this displacement leads to a change in the fluorescent signal:...

  17. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...cannot be deleted, so there is no worry about misplacing your results or losing that lab notebook from...similar apps: Findings Lab Notebook Cell Culture Split Calculator by DRDP Restriction Enzyme Tool by Promega...

  18. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ... is the firefly. Along with charming nighttime displays, these insects have contributed an important tool...systems for examining protein-protein interactions Split luciferases, such as NanoBiT, are expressed in two...

  19. Immunology Research Plasmids and Resources

    Type
    Collection
    ... regulatory factor X-associated protein - SHFM1 split hand/foot malformation (ectrodactyly) type 1 DSS1...oncogene homolog A (avian) MGC131774, NFKB3, p65 SYK spleen tyrosine kinase DKFZp313N1010, FLJ25043, FLJ37489...of sevenless homolog 2 (Drosophila) FLJ25596 SYK spleen tyrosine kinase DKFZp313N1010, FLJ25043, FLJ37489...Fox Foundation is a synuclein phagemid VHH phage display library used to screen for antibodies that bind...

  20. RNA Extraction Without A Kit

    Type
    Blog Post
    ...meant for isolating RNA from rat spleens in which they homogenized spleens in a guanidinium thiocyanate solution...
Showing: 61 - 80 of 238 results