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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 236 results for: SPL

Showing: 61 - 80 of 236 results

  1. Rett Syndrome

    Type
    Collection
    ...RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement to reverse the impacts...2002. Insight into Rett syndrome: MeCP2 levels display tissue- and cell-specific differences and correlate...

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Bacterial vector for Myc tag pETcon(-) - Yeast surface display vector with a C-terminal Myc tag pPMW-attB - pUASp...expression (Gateway) pYD1 - Expression, secretion, and display of proteins on the extracellular surface of S. ...

  3. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...negative effect on these parameters. IT5 and IT6 displayed good binding efficiency in both immunofluorescence...cyclin Cdc28 and the chaperone Ydj1, both of which display sophisticated interactions with multiple other ...

  4. Science as Art

    Type
    Blog Post
    ...Jurassic Technology in LA, where it is currently on display.   Many universities are now following the trend...scientific art competitions where creations can go on display for all to enjoy. The largest competition of this...

  6. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...small (Rep52 and Rep40) Rep proteins. Alternative splicing generates the different C-terminal regions of ...often driven by the AAV p40 promoter. Alternative splicing and differing translational start sites generate...

  8. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...movement. When the ribosome moves along an mRNA, it displaces other RNA-binding proteins to allow translation...translation to occur. For a TRICK reporter mRNA this displacement leads to a change in the fluorescent signal:...

  9. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...cannot be deleted, so there is no worry about misplacing your results or losing that lab notebook from...similar apps: Findings Lab Notebook Cell Culture Split Calculator by DRDP Restriction Enzyme Tool by Promega...

  10. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ... is the firefly. Along with charming nighttime displays, these insects have contributed an important tool...systems for examining protein-protein interactions Split luciferases, such as NanoBiT, are expressed in two...

  13. RNA Extraction Without A Kit

    Type
    Blog Post
    ...meant for isolating RNA from rat spleens in which they homogenized spleens in a guanidinium thiocyanate solution...

  14. Pipetting Protocol

    Type
    Protocol
    ... expel liquid into the pipette tip, the volume display which shows the set volume for the the pipette,...section will help you understand how to read each display to dispense the correct amount of liquid reagent...), while 0.5 µL would read as 050. Each volume display on these pipettes will also have small tick marks...you will be rotating dials behind the numerical display or the top of the plunger of the pipette. Select...

  15. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...generated a photosensitive Cre, Opto-Cre-Vvd, by splitting and joining the protein regions with Vivid (Vvd...low and high-intensity blue light. Unlike other split-Cre systems which can take up to 24 hours to cut...

  16. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...cells with the CRISPR library in vitro before transplanting them into mice. For in vivo screens, mice are...another option is to use variants of Cas9, like split Cas9, or a smaller Cas9 ortholog like SaCas9. You...

  17. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...vitro (see figure 1B in Terskikh et al.) and even displays predictable kinetics over 14 hours of expression... table: Fast-FT, Medium-FT, and Slow-FT. These display progressively longer blue fluorescence maturation...

  18. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...Mapping antibody-escape mutations with the yeast display library. From Starr et al Science., 2021. SARS-CoV... designed so that the SARS-CoV-2 spike RBD is displayed on the yeast cell’s surface. The Bloom lab used...

  20. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...penetration Less resolution due to larger label displacement Require separate incubation of 1° and 2° Must...multiple dyes Greater resolution due to lower label displacement 2° incubation can be skipped by pre-binding ...
Showing: 61 - 80 of 236 results