We narrowed to 249 results for: TIR
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TypeBlog Post... or guide RNA blocking mutations? This depends entirely on the locus you are editing, and if the blocking...experiments with blocking mutations, we still weren’t entirely happy with our editing outcomes and in many cases...
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Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog Post...they can all work at the same temperature, so the entire reaction takes an hour or less to complete at 50...be because the exonuclease could chew through an entire fragment shorter than 200 nucleotides before the... -
Antibodies 101: Validation
TypeBlog Post...and it causes a large deletion that removes the entire exon that codes for the epitope recognized by your...you very similar results, while the third looks entirely different (B), giving you confidence that your... -
A History of Genome Engineering in Popular Culture
TypeBlog Post...and the rise of killer machines that exterminate entire humanity were the two most popular topics that ...negative depictions serve as cautionary tales for the entire scientific community and remind us to always consider... -
Antibodies 101: Introduction to Antibodies
TypeBlog Post...variable region of an antibody does not recognize the entirety of the target protein: instead, it recognizes ...between the IgG antibody and scFv. Because the entire antibody molecule is not necessary for antigen ... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog Post...hypothesized that the CGBE edit process occurs entirely while Cas9 is bound, keeping the two DNA strands...regions that were already accessible. It's not entirely clear why this is, but it might point to a more... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog Post...backbone, sidestepping the problem of HDR vs. NHEJ entirely. The first two classes of base editors were cytosine...base editing, has allowed scientists to replace entire sequences of varying lengths. Base editing publication... -
Using AAV for Neuronal Tracing
TypeBlog Post...and injected at several hundred sites across the entire mouse brain. Traced neurons expressed a fluorescent...close or distal to the injection site across the entire brain, allowing for an unprecedented number of ... -
#DeckTheLab to Win a Tasty Winter Treat This Holiday Season
TypeBlog Post...December are eligible to win a: That will warm up the entire lab (hint: you might need some mini marshmallows... -
'Tis the Season to #DeckTheLab
TypeBlog Post...win a hot chocolate party that will warm up the entire lab. We’ll choose 4 winning submissions and deliver... -
Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More
TypeBlog Post... or on our hot plasmids webpage. Listen to the entire Hot Plasmids podcast! Hot plasmids covered in this... -
Trends in CRISPR and SynBio Technologies
TypeBlog Post...present our own data on plasmid deposits and distirbution for these fast moving fields. Addgene is a ... -
CRISPR 101 eBook...en español
TypeBlog Post...de nuestro repositorio. Este increíble regalo permitirá que más personas accedan a recursos útiles para... -
Celebrating the 2024 Nobel Prize in Chemistry
TypeBlog Post...John Jumper! The Baker Lab focuses on designing entirely new proteins, improving our understanding of how... -
New Podcast Segment: Hot Plasmids
TypeBlog Post...from one of our recent newsletters. Listen to the entire Hot Plasmids episode! We generally publish 5-10... -
Congratulations, Deck The Lab winners!
TypeBlog Post...Vestibular Lab decorated not just their lab, but their entire floor, and put together a festive video to showcase... -
Editor's Choice, July 2016
TypeBlog Post...blog. We achieved roughly 50,000 views over the entire blog and hosted new posts on topics ranging from... -
Fueled by Coffee at #SfN14
TypeBlog Post...cups of coffee would be consumed throughout the entire 5 day event. Though, to be fair, I think this is... -
Plasmid Preps: Different Purity, Different Quantities, Different Uses
TypeBlog Post...chemical contaminants, these DNA samples must also be entirely endotoxin free. Endotoxin is a lipopolysaccharide... -
Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton
TypeBlog Post...cell-based therapies underway. Chapter 7: Generating entire lungs using a decellurized scaffold as a base. ...