... Viral Vector Packaging Service AAV Controls Viral Vector Packaging Service: Control AAV Browse our...our control AAV that express fluorescent proteins under general promoters. These AAV can be used to compare...Fluorophore/Tag Activity Serotype PI 37825 AAV-CAG-GFP CAG GFP Constitutive 1, 2, 5, 8, 9, rg*, PHPeB, CAP-B10...83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Constitutive 1, 2, 5, 8, 9, rg*, PHP.eB Fishell 99130 pAAV-mDlx-...CAG-NLS-GFP CAG NLS-GFP Constitutive PHPeB Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Constitutive...Gradinaru 83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Cre dependent 1, 2, 8, 9, rg* Fishell 83894 pAAV-hDlx-Flex-dTomato-Fishell...options. Browse all Control AAV *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from...

...Cas9. Adenoviral CRISPR vectors (left) or CRISPR vectors and donor DNA vectors (right) are transduced ...larger than AAV vectors. This capacity is large enough to carry the Cas9 gene and a gRNA expression cassette...need to use a two viral vectors system: one vector for Cas9/sgRNA and one vector for your specific DNA ...gRNA and Cas9 using adenoviral vectors As compared to other viral vectors, AdVs can provide a large transgene...adenoviral vectors Since then, other groups have successfully developed and used AdV vectors to edit genes...updated Dec 10, 2020 by Benoit Giquel.   Adenoviral vectors (AdVs) have been used for many years for in vivo...of oncogenicity or genotoxicity seen with other vectors (Lukashev et al., 2016). The protein capsid encapsulating...

...transient expression vector GFP Varies pMKO.1 GFP - Retroviral shRNA expression pLenti CMV...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...lentiviral vector for ORF expression pDest-565 - Gateway destination vector for bacterial expression; His,GST...pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression Fluorescent proteins (GFP, mCherry...gene expression vector URA3 Yeast pAG306GAL-ccdB - Yeast Gateway expression vector (see...protein 35S-eGFP-nosT - Transient expression vector for eGFP in plants Find FP tagging vectors in budding yeast...lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression pLJM1-EGFP - 3rd gen lentiviral...

...CMV AAV expression of firefly luciferase and GFP James Wilson 22522 phGluc Gaussia EF1α Expression of ...Lentiviral expression of firefly luciferase Paul Khavari 109395 EFS-Vector Firefly EF1α AAV-CRISPR knockout...Firefly TGB AAV expression of firefly luciferase and GFP James Wilson 106457 pCR3.1-Luc Firefly CMV Mammalian...Lentiviral expression of firefly luciferase with a V5 tag Kevin Janes 83281 pAAV-CAG-FLuc Firefly CAG AAV expression...luciferase Mark Kay 105532 pAAV.CMV.ffLuciferase.SV40 Firefly CMV AAV expression of firefly luciferase James...transfection Sidi Chen 118412 ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293 Firefly EF1α AAV expression of firefly luciferase...Zea mays expression of firefly luciferase Paul Schulze-Lefert 83282 pAAV-CAG-RLuc Renilla CAG AAV expression...

...Viral Vector Packaging Service AAV Recombinases Viral Vector Packaging Service: Recombinases Cre AAV Dre...Dre AAV Flpo AAV Light-Inducible Recombinases VCre AAV Browse the following tables for in-stock AAV preps...107788 AAV.rTH.PI.Cre.SV40 rTH none 9, rg* Wilson 24593 AAV-pgk-Cre PGK none rg* Aebischer 51507 AAV pmSyn1...provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of site...Fluorophore Serotype(s) PI 50363 AAV phSyn1(S)-DreO-bGHpA Syn none 5, rg* Zeng Flpo AAV ID Name Promoter Fluorophore...from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our...55638 pAAV-EF1a-vCre EF1a none 8, rg* Deisseroth *AAVrg = retrograde serotype, produced with the AAV retro...

... in E3 and insertion of GFP expression cassette. Bunz 179202 pAd5-B6/7 ∆E3-GFP Adenoviral A block for ...Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are a class of...regions present in pAd5-B6 ∆E3-GFP and pAd5-B7 . Bunz 179203 pAd5-B1-deltaE1-GFP Adenoviral A block for the...Block 1 with deletion in E1 and insertion of GFP expression cassette. Bunz 16402 pShuttle Shuttle For production...production of GFP-trackable viruses containing transgene under CMV promoter Vogelstein 16407 pAdEasy 2-GFP beta-gal...Collection of adenovirus shuttle vectors and strains Viral...kb), high titers, and high levels of transgene expression. Additionally, these viruses are able to target...

...western blot for the myc-tagged protein GFP-smFP-myc expressed from Plasmid 98926. See the Applications...available as Cre-dependent, EF1ɑ-driven viral vectors (AAV1) - and you can also find several other constructs...B) Cortical slice of HcKCR1-EYFP and tdTomato expressed layer 2/3 neurons in mouse. C) Action potential...voltage traces of action potentials in a HcKCR1-expressing mouse neuron without (top) and with (bottom) ...

...Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro...MSCV-IRES-GFP MSCV For transgene expression and GFP marker Reya 21654 pMSCV PIG (Puro IRES GFP empty plasmid...also expresses luciferase Lowe 9044 pMIG MSCV Plasmid for transgene expression; also expresses GFP. Also .... Hahn 63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in mammalian...gene expression; select with puromycin or screen for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-...fused to eGFP expression Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression; also ...Guide Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus...

...Addgene.org Browse Our Viral Vector Plasmids Read Our Lentiviral Vector Guide Read Our AAV Guide  ...producing infectious virus (similar to lentiviral vectors) because they do not encode all of the necessary...plasmids allows researchers to partially control expression levels of each gene by altering the amount transfected...RNA polymerase gene or using cells that stably express T7 (e.g. BSR T7/5 cells) (1). We hope that minigenomes...on the Addgene Blog Read Other Posts on Viral Vectors Check out Our Scientist's Guide to Ebola Contribute... viral vector...non-viral) reporter gene (e.g. firefly luciferase or eGFP) flanked by the EBOV-specific gene start and gene...

...Delivery method Expression System Components of System Application Mammalian expression vector Cas enzyme ...lentiviral transfer vector or separate transfer vectors May contain reporter gene (e.g. GFP) or selection marker... using CRISPR AAV transduction CRISPR elements are inserted into an AAV transfer vector and used to generate...system. Select Expression System To use CRISPR, you will need both Cas9 and a gRNA expressed in your target...to the transient Cas9 expression. The table below summarizes the major expression systems and variables...details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or gRNA in a mammalian...generate AAV particles (for details, see our AAV Guide ) ∼4.5 kb packaging limit (only compatible with...

... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your...constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 ...size and allow for expression of more sgRNAs in a single vector. sgRNAs are expressed as polycistronic ... pCAG-SpCas9-GFP-U6-gRNA 79144 Mammalian hU6 yes, cut S. pyogenes EGFP Zou pCAG-eCas9-GFP-U6-gRNA 79145...gRNA expression vectors for insertion of custom gRNA target sequences. CRISPR...CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut Base...based on: Your particular expression system , eg. Mammalian, Lentiviral, AAV, Bacteria, Drosophila, Yeast...

...the vector backbone you cloned your insert into and fill out the appropriate boxes with the vector backbone...backbone manufacturer, vector backbone size, total size of your vector + insert, any modifications you ...insert in E. coli, then this is a Bacterial Expression vector. If your plasmid can be classified in multiple...lentiviral vector to be used in mammalian cells, check Lentiviral and Mammalian Expression). This information... location, while shRNAs are used to knockdown expression of mRNAs with complementarity to the shRNA sequence...provide any full or partial plasmid sequences and any vector maps. The more sequence data available, the better... was a part of the insert (when cloned into the vector backbone). If your plasmid has more than one insert...

...gene expression at a single copy in mammalian cells; Expresses firefly luciferase hairpin and GFP under...option rtTA On Kowarz 16623 pBI-GFP Expression of your gene of interest & GFP from a bidirectional tet-responsive... None Either Elledge 35625 pAAV-Ptet-RFP-shR-rtTA AAV; shRNA cloning vector; for evaluation of shRNA efficacy...integration into AAVS1 Genomic Safe Harbor Locus. Tet-On 3G On Doyon 58245 pGLTR-X-GFP Single vector lentiviral...Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Additional Resources: Browse...subsequent increase in expression by the activation domain, resulting in reduced gene expression. This idea of ... rtTA or TetR expression plasmid Stable cell lines can be made that continuously express a system component...

...: Viral Service: All AAV Viviana Gradinaru PHP AAV Serotypes Blog: Viral Vectors As part of our Viral ...Cynomolgus Macaques of rAAV2tYF-CB-hRS1, a Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin.... Viral Vector Packaging Service AAV University of Florida Serotype Testing...viral production , these viral vectors undergo quality control, including AAV titration by ddPCR, in vitro...full sequencing of the final viral vector preparation. These viral vector preparations were produced with...transduction of the mouse retina by tyrosine-mutant AAV serotype vectors. Mol Ther . 2009 Mar;17(3):463-71. PMID:...Rodriguez-Estevez, et al. 2020. Transduction optimization of AAV vectors for human gene therapy of glaucoma and their ...

... Viral Vector Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor AAV Browse our...pAAV-Syn-Archon1-KGC-GFP-ER2 Syn Archon1 EGFP Constitutive 8 Boyden 115893 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] Syn... our AAV that express fluorescent biosensor proteins, including calcium sensors, under the control of ...162376 pGP-AAV-syn-jGCaMP8f-WPRE Syn jGCaMP8f none Constitutive 1, 9, rg* GENIE 162379 pGP-AAV-syn-FLEX-...162382 pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent 1, 5, 9, rg* GENIE 169258 AAV-hSyn-Soma-jGCaMP8f...176750 AAV-CamKIIa-jGCaMP8f-WPRE CamKIIa jGCaMP8f none Constitutive 1, 5, rg* Looger 176753 AAV-mDlx-jGCaMP8f-WPRE...162374 pGP-AAV-syn-jGCaMP8s-WPRE Syn jGCaMP8s none Constitutive 1, 5, 9, rg* GENIE 162377 pGP-AAV-syn-FLEX-jGCaMP8s-WPRE...

... Nat Biotechnol. 2023 May 22. See More AAV Viral Preps Find AAV viral preps for systemic delivery of viral...CBA-driven, Cre-dependent expression of diphtheria toxin receptor (DTR)–GFP fusion protein. Azim et al...inducible vector . Harley et al. Cell Rep. 2023 Dec 26. Induce CNS cells with concomitant expression of aggregation-prone...research. Disease Info Plasmid Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors Antibodies...Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant ORFs, gene fragments, and...to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and more. New...Oct 18. Target neural oxytocin receptors using an AAV-CRISPR/Cas9 strategy for gene editing across divergent...

...Questions (FAQ) about Adenoviral Vectors Are adenovirus and AAV different? Yes! AAV is a small single-stranded... Ad5-based vector Most common type of adenoviral vector pAdTrack Class of transfer vectors that contain...contain IRES-GFP pShuttle Class of transfer vectors that do not contain GFP Additional Vocabulary Term Definition...adenovirus types, and most adenoviral vectors are based on Ad5. Ad5-based vectors use the Coxsackie-Adenovirus ...adenoviral vector constructs. The system consists of two types of plasmids: shuttle (or transfer) vectors and...and adenoviral vectors. Find vectors for this system on our adenovirus plasmid page. The transgene of interest...multiple shuttle vectors for different purposes. The pAdTrack series contains an IRES-GFP construct that...

... Viral Vector Packaging Service AAV Monosynaptic Neuronal Tracing Viral Vector Packaging Service...virus. AAV Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G... Tracing These AAV encode tools for rabies virus-based monosynaptic tracing. These AAV can be used with...cells engineered to express TVA will be infected. Schematic depicting steps for using AAV and modified rabies...from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our...pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used to complement deletion-mutant rabies virus AAV1 Wickersham...Ready-to-use AAV available from Addgene's viral service encoding tools for monosynpatic retrograde neuronal...

...Douglas Kim , GENIE Project Calcium Bicistronic vector expressing GCaMP6 and mRuby2 for imaging neural activity...Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca+ dynamics in endoplasmic reticulum GFP-Aequorin Protein...2018 Oct 25;9(1):4440. Eric Schreiter Calcium AAV expression of photoconvertible fluorescent protein-based...2024.12.16.628673. Olivia Masseck Calcium Astrocyte AAV-mediated expression of jRCaMP1a, a red fluorescent calcium ...FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based ...Localization Optogenetics Plasmids Viral Service: AAV Biosensors Fluorescent biosensors are proteins designed...associated with the article. We also offer ready-to-use AAV preparations of select plasmids; these are noted ...

...Delivery method Expression System Components of System Application Mammalian expression vector Cas enzyme ...lentiviral transfer vector or separate transfer vectors May contain reporter gene (e.g. GFP) or selection marker... using CRISPR AAV transduction CRISPR elements are inserted into an AAV transfer vector and used to generate...system. Select Expression System To use CRISPR, you will need both Cas9 and a gRNA expressed in your target...to the transient Cas9 expression. The table below summarizes the major expression systems and variables...details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or gRNA in a mammalian...generate AAV particles (for details, see our AAV Guide ) ∼4.5 kb packaging limit (only compatible with...