Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 84 results for: aav vectors expressing gfp

Showing: 61 - 80 of 84 results

  1. Serotype Testing AAV

    Type
    Collection
    ...Service AAV Serotype Testing AAV Viral Vector Packaging Service: Serotype Testing AAV These AAV encode ...the suffix (for example, AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description...Ready-to-use AAV available from Addgene's viral service. Control EGFP vectors in various serotypes for...serotype testing: AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV11, and AAV retrograde. Viral.... These AAV are made from plasmid 50465 (deposited by Bryan Roth ) and direct EGFP expression from the...experiments. For more information, see our full AAV inventory or our AAV production page . Note: For a list of ...Description : Ready-to-use AAV in various serotypes suitable for direct injection. These AAV are made from plasmid...

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...transient expression vector GFP Varies pMKO.1 GFP - Retroviral shRNA expression pLenti CMV...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...lentiviral vector for ORF expression pDest-565 - Gateway destination vector for bacterial expression; His,GST...pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression Fluorescent proteins (GFP, mCherry...gene expression vector URA3 Yeast pAG306GAL-ccdB - Yeast Gateway expression vector (see...protein 35S-eGFP-nosT - Transient expression vector for eGFP in plants Find FP tagging vectors in budding yeast...lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression pLJM1-EGFP - 3rd gen lentiviral...

  3. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...Cas9. Adenoviral CRISPR vectors (left) or CRISPR vectors and donor DNA vectors (right) are transduced ...larger than AAV vectors. This capacity is large enough to carry the Cas9 gene and a gRNA expression cassette...need to use a two viral vectors system: one vector for Cas9/sgRNA and one vector for your specific DNA ...gRNA and Cas9 using adenoviral vectors As compared to other viral vectors, AdVs can provide a large transgene...adenoviral vectors Since then, other groups have successfully developed and used AdV vectors to edit genes...updated Dec 10, 2020 by Benoit Giquel.   Adenoviral vectors (AdVs) have been used for many years for in vivo...of oncogenicity or genotoxicity seen with other vectors (Lukashev et al., 2016). The protein capsid encapsulating...

  4. Tetracycline Inducible Expression

    Type
    Collection
    ...Ting 85040 pK170.AAV-TRE-Cre-WPRE (Supernova) AAV vector for dox-inducible expression of Cre recombinase...Iwasato 63704 pRetroX GFP T2A Cre Retroviral vector for dox-inducible expression of GFP T2A Cre recombinase...Eric Kowarz 96930 XLone-GFP Tet-On PiggyBac vector for inducble expression of EGFP Tet-On 3G rtTA TRE3GS...171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression of EGFP Tet-On 3G rtTA TRE3GS...Tet-On 3G rtTA David Vereide 120309 pAAV-FAH-rtTA3G AAV vector to express Tet-On 3G transactivator under the...-On vector expressing TetR from CMV promoter TetR Eric Campeau , Paul Kaufman 175274 pAAV-rtTA AAV Tet-On... rtTA. tTA Edward Hsiao 99118 pAAV-CAG-tTA AAV Tet-Off vector, expresses tTA from the CAG promoter. See...

  5. Luciferase Plasmid Collection

    Type
    Collection
    ...CMV AAV expression of firefly luciferase and GFP James Wilson 22522 phGluc Gaussia EF1α Expression of ...pscAAV-CAG-RLuc Renilla CAG Vector for packaging self-complementary AAV expressing Renilla luciferase Mark Kay 140328...Schulze-Lefert 83282 pAAV-CAG-RLuc Renilla CAG AAV expression of Renilla luciferase Mark Kay 83280 pscAAV-CAG-RLuc...Lentiviral vector with dox- or tet-inducible luciferase expression Stephen Tapscott 83281 pAAV-CAG-FLuc ...Firefly CAG AAV expression of firefly luciferase Mark Kay 105532 pAAV.CMV.ffLuciferase.SV40 Firefly CMV...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...a reference level of expression. Browse or search for popular plasmids expressing luciferase. We also ...

  6. Adenovirus Plasmids

    Type
    Collection
    ... in E3 and insertion of GFP expression cassette. Bunz 179202 pAd5-B6/7 ∆E3-GFP Adenoviral A block for ...Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are a class of...regions present in pAd5-B6 ∆E3-GFP and pAd5-B7 . Bunz 179203 pAd5-B1-deltaE1-GFP Adenoviral A block for the...Block 1 with deletion in E1 and insertion of GFP expression cassette. Bunz 16402 pShuttle Shuttle For production...production of GFP-trackable viruses containing transgene under CMV promoter Vogelstein 16407 pAdEasy 2-GFP beta-gal...Collection of adenovirus shuttle vectors and strains Viral...kb), high titers, and high levels of transgene expression. Additionally, these viruses are able to target...

  7. Retrovirus Plasmids

    Type
    Collection
    ...Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro...MSCV-IRES-GFP MSCV For transgene expression and GFP marker Reya 21654 pMSCV PIG (Puro IRES GFP empty plasmid...also expresses luciferase Lowe 9044 pMIG MSCV Plasmid for transgene expression; also expresses GFP. Also .... Hahn 63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in mammalian...gene expression; select with puromycin or screen for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-...fused to eGFP expression Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression; also ...Guide Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus...

  8. Hot Plasmids - October 2022

    Type
    Blog Post
    ...western blot for the myc-tagged protein GFP-smFP-myc expressed from Plasmid 98926. See the Applications...available as Cre-dependent, EF1ɑ-driven viral vectors (AAV1) - and you can also find several other constructs...B) Cortical slice of HcKCR1-EYFP and tdTomato expressed layer 2/3 neurons in mouse. C) Action potential...voltage traces of action potentials in a HcKCR1-expressing mouse neuron without (top) and with (bottom) ...

  9. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Mammalian Expression sfGFP-C1 - Mammalian Expression sfGFP-pBAD - Bacterial Expression moxGFP 486 510 50 17 min...dimerization pcDNA3-EGFP - Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral...Mammalian Expression mEmerald-C1 - Mammalian Expression mEmerald-pBAD - Bacterial Expression EGFP 488 507...mGreenLantern - Mammalian Expression pAAV-CAG-mGreenLantern - Mammalian AAV Expression pBAD-mGreenLantern -...pcDNA-miRFP670nano3 - Mammalian Expression pAAV-hSYN-miRFP670nano3-WPRE-hGHpA - Mammalian AAV Expression pBAD/His-miRFP670nano3...pBad-EBFP2 - Bacterial Expression EBFP2-N1 - Mammalian Expression EBFP2-C1 - Mammalian Expression EBFP2-pBAD - ...- Mammalian Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian...

  10. Recombinases AAV Preps

    Type
    Collection
    ...Viral Vector Packaging Service AAV Recombinases Viral Vector Packaging Service: Recombinases Cre AAV Dre...Dre AAV Flpo AAV Light-Inducible Recombinases VCre AAV Browse the following tables for in-stock AAV preps...provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of site...rg* James M. Wilson 24593 AAV-pgk-Cre PGK none rg* Patrick Aebischer 51507 AAV pmSyn1-EBFP-Cre Syn EBFP...Serotype(s) PI 50363 AAV phSyn1(S)-DreO-bGHpA Syn none 5, rg* Hongkui Zeng Flpo AAV ID Name Promoter Fluorophore...with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory...from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our...

  11. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...Addgene.org Browse Our Viral Vector Plasmids Read Our Lentiviral Vector Guide Read Our AAV Guide  ...producing infectious virus (similar to lentiviral vectors) because they do not encode all of the necessary...plasmids allows researchers to partially control expression levels of each gene by altering the amount transfected...RNA polymerase gene or using cells that stably express T7 (e.g. BSR T7/5 cells) (1). We hope that minigenomes...on the Addgene Blog Read Other Posts on Viral Vectors Check out Our Scientist's Guide to Ebola Contribute... viral vector...non-viral) reporter gene (e.g. firefly luciferase or eGFP) flanked by the EBOV-specific gene start and gene...

  12. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your...constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 ...size and allow for expression of more sgRNAs in a single vector. sgRNAs are expressed as polycistronic ... pCAG-SpCas9-GFP-U6-gRNA 79144 Mammalian hU6 yes, cut S. pyogenes EGFP Zou pCAG-eCas9-GFP-U6-gRNA 79145...gRNA expression vectors for insertion of custom gRNA target sequences. CRISPR...CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut Base...based on: Your particular expression system , eg. Mammalian, Lentiviral, AAV, Bacteria, Drosophila, Yeast...

  13. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101...using AAV vectors. Highlighted Plasmids Browse the tables below to find popular plasmids expressing your... plasmids expressing Cre recombinase and filter the results by expression system and vector type. Find... plasmids expressing Flp recombinase and filter the results by expression system and vector type. Find... plasmids expressing Dre recombinase and filter the results by expression system and vector type. Find...genome or control gene expression or skip ahead to browse highlighted plasmids expressing Cre , Flp , Dre ,...Recognition Sites. Controlling Recombinase Expression By expressing the enzyme at specific times or locations...

  14. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...the vector backbone you cloned your insert into and fill out the appropriate boxes with the vector backbone...backbone manufacturer, vector backbone size, total size of your vector + insert, any modifications you ...insert in E. coli, then this is a Bacterial Expression vector. If your plasmid can be classified in multiple...lentiviral vector to be used in mammalian cells, check Lentiviral and Mammalian Expression). This information... location, while shRNAs are used to knockdown expression of mRNAs with complementarity to the shRNA sequence...provide any full or partial plasmid sequences and any vector maps. The more sequence data available, the better... was a part of the insert (when cloned into the vector backbone). If your plasmid has more than one insert...

  15. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...: Viral Service: All AAV Viviana Gradinaru PHP AAV Serotypes Blog: Viral Vectors As part of our Viral ...Cynomolgus Macaques of rAAV2tYF-CB-hRS1, a Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin.... Viral Vector Packaging Service AAV University of Florida Serotype Testing...viral production , these viral vectors undergo quality control, including AAV titration by ddPCR, in vitro...full sequencing of the final viral vector preparation. These viral vector preparations were produced with...transduction of the mouse retina by tyrosine-mutant AAV serotype vectors. Mol Ther . 2009 Mar;17(3):463-71. PMID:...Rodriguez-Estevez, et al. 2020. Transduction optimization of AAV vectors for human gene therapy of glaucoma and their ...

  16. Biosensor AAV Preps

    Type
    Collection
    ... Viral Vector Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor AAV Browse our...pAAV-Syn-Archon1-KGC-GFP-ER2 Syn Archon1 EGFP Constitutive 8 Boyden 115893 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] Syn... our AAV that express fluorescent biosensor proteins, including calcium sensors, under the control of ...162376 pGP-AAV-syn-jGCaMP8f-WPRE Syn jGCaMP8f none Constitutive 1, 9, rg* GENIE 162379 pGP-AAV-syn-FLEX-...162382 pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent 1, 5, 9, rg* GENIE 169258 AAV-hSyn-Soma-jGCaMP8f...176750 AAV-CamKIIa-jGCaMP8f-WPRE CamKIIa jGCaMP8f none Constitutive 1, 5, rg* Looger 176753 AAV-mDlx-jGCaMP8f-WPRE...162374 pGP-AAV-syn-jGCaMP8s-WPRE Syn jGCaMP8s none Constitutive 1, 5, 9, rg* GENIE 162377 pGP-AAV-syn-FLEX-jGCaMP8s-WPRE...

  17. Neurodegeneration Research Collection

    Type
    Collection
    ... Nat Biotechnol. 2023 May 22. See More AAV Viral Preps Find AAV viral preps for systemic delivery of viral...CBA-driven, Cre-dependent expression of diphtheria toxin receptor (DTR)–GFP fusion protein. Azim et al...inducible vector . Harley et al. Cell Rep. 2023 Dec 26. Induce CNS cells with concomitant expression of aggregation-prone...research. Disease Info Plasmid Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors Antibodies...Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant ORFs, gene fragments, and...to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and more. New...Oct 18. Target neural oxytocin receptors using an AAV-CRISPR/Cas9 strategy for gene editing across divergent...

  18. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    .... We also offer ready-to-use AAV preparations of select plasmids and AAV Packaged on Request for many ...Douglas Kim , GENIE Project Calcium Bicistronic vector expressing GCaMP6 and mRuby2 for imaging neural activity...Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca+ dynamics in endoplasmic reticulum GFP-Aequorin Protein...2018 Oct 25;9(1):4440. Eric Schreiter Calcium AAV expression of photoconvertible fluorescent protein-based...2024.12.16.628673. Olivia Masseck Calcium Astrocyte AAV-mediated expression of jRCaMP1a, a red fluorescent calcium ...FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based ...Luciferase Plasmids Optogenetics Plasmids Viral Service: AAV Biosensors Fluorescent biosensors are proteins designed...

  19. AAV for Neuronal Tracing

    Type
    Collection
    ... Viral Vector Packaging Service AAV Monosynaptic Neuronal Tracing Viral Vector Packaging Service...virus. AAV Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G... Tracing These AAV encode tools for rabies virus-based monosynaptic tracing. These AAV can be used with...cells engineered to express TVA will be infected. Schematic depicting steps for using AAV and modified rabies...from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our...100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used to complement deletion-mutant rabies...Ready-to-use AAV available from Addgene's viral service encoding tools for monosynpatic retrograde neuronal...

  20. Adenovirus Guide

    Type
    Guide
    ...Viral Vectors Adenoviral Vector Guide Adenoviral Vector Guide Jump To: Adenoviral Vectors Vector Production...capacity of rAdV vectors (~6.5 kb for first-generation rAdV vectors) surpasses that of rAAV vectors (~4.7 kb)...more information about AAVs, read our AAV guide . Recombinant Adenoviral Vectors Wild-type adenoviruses...pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene of interest...shuttle/transfer vectors for recombinant adenoviral vector production that contain IRES-GFP. Pseudotyping...shuttle/transfer vectors for recombinant adenoviral vector production that do not contain GFP. Recombinant...Uses of Adenoviral Vectors Although recombinant adeno-associated viral (rAAV) vectors are often the preferred...
Showing: 61 - 80 of 84 results