...s podcast, we sit down with Wei Leong Chew, a researcher at the Genome Institute of Singapore who recently...to go into genome engineering. Chapter 1: Early research on CRISPR and what it's like to work in a competitive...Genome Institute of Singapore. Chapter 3: Biology research and resources in Singapore. Chapter 4: Life as... AAVs, and more. Chapter 7/Outro: Advice for researchers pursuing careers in academia. Many thanks to...Listen to our interview with synthetic biology researcher Tom Ellis Resources at Addgene.org Find CRISPR...

...writer at the non-profit Fred Hutchinson Cancer Research Center. Susan Keown’s science communication journey...Eye Institute (NEI), where she wrote about NEI research for a general audience. She then got a full time...it.” Science writing at Fred Hutchinson Cancer Research Center Work as a Science Writer at the Hutch ...meetings and Susan is assigned to cover the Clinical Research Division at Fred Hutch, so she attends that division...writing: plain-language stories about Fred Hutch research, pieces for donors such as fundraising materials...did for the Northwest Association of Biomedical Research. She does, however, recommend using Twitter as...

...your own company, work in a bench research career or a non-bench research career such as law, medical/technical...nonprofit entity, or nonprofit research organizations or governmental research organizations. Since there ...Law has seen that research institutions are willing to sponsor postdoctoral researchers for the EB-1B using...part of career development for foreign national researchers and entrepreneurs who are looking to continue...first 3 quarters of 2017. As an international researcher and aspiring entrepreneur, you have many career...complete your Ph.D. or postdoc. Foreign national researchers who require visas, permanent residence or citizenship...aligned with their education/experience, i.e. a research role, for a Ph.D. trained scientist. If the candidate...

...different labs and experimental systems in their own research. Cyanobacteria, a phylum of photosynthetic bacteria...

...plasmid pET21a-BirA.   DNA / protein database search and analysis tools Addgene's analyze sequence ...

...publications. Google Image Search Google image search is a great tool for searching the entire internet for...you can search for images “Labeled for reuse with modification.” Occasionally Google Image Search results...illustrators with input from life scientists. It’s easy to search the collection and drag and drop an icon onto the...graphics. The tools settings let you tailor your search to particular types of images. For example, by ...resources on the Addgene blog Read our Early Career Researcher Toolbox blog post on social media for scientists...

...with Seeding Labs to provide plasmid grants to researchers in developing countries. Today we’re proud to... to use plasmids from Addgene to advance their research. Louis Bengyella Dr. Bengyella’s work has focused... As previously, these awards will be open to researchers who have received equipment through Seeding Labs... Addgene Blog Learn about Plasmid Awards to Researchers Working on Rare Diseases Read About Some of Our... search...

...Researchers in developing countries often find themselves on the front lines of emerging global challenges...challenges. However, these same researchers face significant barriers to scientific discovery and career ... see it as part of our mission to enable all researchers and are proud to announce that we are teaming...opportunities and scientific resources available to researchers in developing countries--because scientific talent...supply surplus lab equipment to carefully-screened research and teaching departments at universities around...working with Seeding Labs to award plasmids to research labs that have received Instrumental Access equipment... these plasmids will effectively advance their research. The map below shows all the countries that have...

...further contribute to our mission to “accelerate research and discovery.” Here are the details. Plasmids...include 10 plasmids that can be directly used in the research on the SARS-CoV-2. Nine of the overall plasmid...

... team identified Archon1, a novel opsin-based fluorescent voltage indicator. Archon1 can embed itself ...compatible with optogenetic control. The researchers also showed that Archon1 is functional in a wide range of...activity using the fluorescent voltage indicator Archon Article contributed by Shreya Vedantam  Listen ...membrane where cell voltage can then be measured. Archon1 is  bright, exhibits good localization, has high...models such as mice, C.elegans, and Zebrafish. Find Archon1 plasmids at Addgene! Piatkevich KD et al, Nat Chem...

...assign four different barcodes to each of the gRNAs and to embed these barcodes within the gRNA sequences...method that makes use of guide RNAs with internal barcodes (iBARs) in their loop region. After infecting ...Predictive Activity through Recombinase Competition (SPARC) is a fully genetic tool for labeling just a fraction...titrations of AAV or heat-shocking the organism. The SPARC system uses competitive recombination sites to selectively...labeled), intermediate (12%), and sparse (4%) levels. SPARC is a versatile system that can be used in many cell...interest in the cells you are studying. Read our SPARC blog post for more details. Figure 2: Dense...

... electro-competent E. coli - excess salt causes arcing during electroporation which would cause the electroporation...into a beta-galactosidase cassette allowing a researcher to perform blue-white screening after transformation...

...associated with neuronal activity, such as Fos, Egr3, and Arc. Thus, CRISPRa represents a powerful method to dissect...commentary on their application to neuroscience research. What is CRISPR-based activation? To summarize...immunohistochemistry, it is not amenable to FACS.  Thus, the researchers inserted human CD8, a gene that encodes a surface...

...Fos locus Mammalian Luo 46389 pArcCreER Cre-ERT2;Targeting vector for Arc locus Mammalian Luo 47117 pT3TS-Cre...recombination of LoxP flanked DNA sequences. Use the search box below to find the perfect Cre expression construct...construct for your experiment. You can search the table for the promoter, fusion, or expression system...Sauer 11919 pBS448 RSV-GFPcre Cre-GFP fusion Rous sarcoma virus (RSV) LTR/promoter Mammalian Sauer 11920 ...Ryffel 31132 pCSCre Cre CMV Xenopus Ryffel 31309 pCARCre Cre cardiac actin Xenopus Ryffel 32144 pJFRC170...

...Methanogenic archaeon Bacterial Ahmed Badran 209739 pAB228i24 G1 PylRS Methanogenic archaeon Bacterial Ahmed...Elsaesser 154763 pAS_FLAG-G1 PylRS G1 PylRS M. archaeon Mammalian TAG Simon Elsaesser 154764 pAS_FLAG-...154768 pAS_4xG1 PylT FLAG-G1 PylRS G1 PylRS M. archaeon Mammalian TAG Simon Elsaesser 154769 pAS_4xG1 ...4xG1 PylT FLAG-G1 PylRS Y125A G1 PylRS Y125A M. archaeon Mammalian TAG Simon Elsaesser 154773 pAS_4xhybPylT...A41AA C55A FLAG-G1 PylRS Y125A G1 PylRS Y125A M. archaeon Mammalian TAG Simon Elsaesser 154774 pAS_4xU6-... Jason W Chin 174718 pRSF-G1mCNPRS G1mCNPRS M. archaeon L-3-(2-cyano-4-pyridyl)alanine (mCNP) Bacterial... Thomas Huber 174719 pRSF-G1pCNPRS G1pCNPRS M. archaeon L-3-(2-cyano-5-pyridyl)alanine (pCNP) Bacterial...

...protein mutations. Research Articles : A list of free access articles on COVID-19 research. Protocols : Protocols...can be found here: ASM COVID-19 Research Registry COVID-19 Open Research Dataset (CORD-19) LitCovid (NCBI...plasmids and resources available for Coronavirus research. Collections...and Coronavirus Plasmids & Resources The global research community is moving quickly to expand the knowledge...available that may be useful for COVID-19 related research. Additionally, we have linked to collections of...Protocols that may be useful for COVID-19 research. Antibodies Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17...available as soon as possible. For a full list of searchable plasmids, see the table below. SARS-CoV-2 Viral...

...Neuroendocrine Tumor Research Foundation The mission of the Neuroendocrine Tumor Research Foundation (NETRF...window) is to fund research to discover cures and more effective treatments for carcinoid, pancreatic, and...Neuroendocrine Tumor Research Foundation Depositor... Depositor Collections Neuroendocrine Tumor Research Foundation Neuroendocrine...creating and maintaining a highly collaborative NET research community is pivotal in accelerating progress ...materials and resources available to academic researchers around the world. The NETRF is grateful for the... Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou Harvard University Epigenetic...

...possible function of a protein of interest. Browse or search the table below to find markers of general cellular...and COX VIII signal peptide dL5 FAP; mCerulean3 Marcel Bruchez 44385 pLV-mitoGFP Mitochondria COX8 GFP...Nucleus NLS (from Mak16p protein) dL5 FAP; mCerulean3 Marcel Bruchez 73207 pcDNA3.1-KozATG-dL5-2XG4S-mCer3 Cytosol... Cytosol, nucleoplasm None dL5 FAP; mCerulean3 Marcel Bruchez 36206 pmTurquoise2-NES Non-nucleus Nuclear...surface (mammalian) PDGFR-derived dL5 FAP; mCerulean3 Marcel Bruchez 45944 pTDpelB-C_sfYFPTwinStrep Periplasmic...peroxisome transport signal dL5 FAP; mCerulean3 Marcel Bruchez 85065 pmScarlet-I_peroxisome_C1 Peroxisome... Cooper *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein...

...different types of viruses that are commonly used in research, and to find plasmids that can be used to engineer...engineer viruses for a wide array of research purposes. Ready-to-use viral preparations for select plasmids...virus-mediated delivery has many advantages for research. Viruses facilitate the delivery of genetic information... (left). B) Viral plasmids can be modified for research purposes such that, when delivered to a host, ...but instead encode for a specific gene or other research interest. To produce viruses with alternate (non-virus-producing...giving rise to more virus (as happens in nature), researchers can introduce plasmids to a host to generate ...harbor a wide array of viral genomes, enabling researchers to direct a wide array of genetic functions in...

...AAV-FLEX-Arch-GFP CAG Arch GFP Cre dependent 1, 9 Boyden 28305 pAAV-FLEX-ArchT-tdTomato CAG ArchT tdTomato...Foff 2.0-Arch3.3-EYFP nEF Arch3.3 EYFP Cre dependent 8 Deisseroth 137150 pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP...dependent 5 Boyden 99039 pAAV-CamKII-ArchT-GFP (PV2527) CaMKII ArchT GFP Constitutive 1, 5, 9, rg* Boyden...Boyden 137148 pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP nEF Arch3.3 EYFP Cre and Flp dependent 8 Deisseroth 137149...Chrimson, ChRmine, ChroME, ChETA, C1V1 (t/t) Inhibitory Arch and variants eNpHR and variants Jaws stGtACR iC+...p2a-EYFP nEF Arch3.3 EYFP Flp dependent 8 Deisseroth 20949 pAAV-double floxed-eNpHR-EYFP-WPRE-pA EF1a eNpHR...