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We narrowed to 494 results for: control

Showing: 61 - 80 of 494 results

  1. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ... to neuroscience. In fact, precise light-based control of biological processes is highly useful in other... to be optimized by Tabor’s lab, making light-controlled gene expression in E. coli easier and more robust...engineered to deliver precise, consistent, and controllable pulses of light. Complicated patterns of light...new pathways with sophisticated gene expression controls. Most cell types do not respond to low-intensity...two systems can be coexpressed for sophisticated control of gene expression. An E. coli light-sensitive...from this publication at Addgene. Multichromatic control of gene expression in Escherichia coli. Tabor JJ...

  2. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...video making.   Cycle One: The Ironing Board Control Center With the pandemic in full swing and everyone...Ironing boards. Good as a WFH desk, and as a video control center.   Cycle 2: Zooming Forward Design: ...me to set up a camera in a room, but still have control over focus, zoom, stop/start record, and allow ...focus system. Follow focus systems allow you to control the focus or zoom on a camera lens without having...door (connected via bluetooth to the camera, to control some basic functions, including starting and stopping... complete with wireless video receiver, camera control, and extra hand sanitizer.    Cycle 4: Venturing...mount a battery to it if necessary. I could still control the camera from a distance if I needed to be close...

  4. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ... APC control, with APC and APC-CY7 plotted against each other. Since it is a single-color control, you...example with single color control. Flow plots showing an APC single-color control sample before (A) and after...recommended to start compensating with single-color controls before moving onto your experimental samples. ...samples without needing to use your single-color controls as a starting point. Applying compensation to ...experimental samples as they did with your single control samples: if the population needs to be shifted ...

  6. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...protein. With this control set, you should see your target protein IP’d in the positive control sample but not...permanently altering the structure of the protein.  Controls As with any experiment, it is critical to include...include proper positive and negative controls for an IP. When possible, perform the IP in parallel with ...interest and a sample that does not. Common negative controls include knockout cell lines or tissues that do... not in the negative control, confirming that the capture antibody is functioning as expected. In order...perform parallel IPs with beads only and an isotype control antibody. Alternative applications Oftentimes, ...

  7. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...derived from the control trace file. On the plot, the green (edited sample) and orange (control sample) lines...cells, genomic DNA from both edited and unedited (control) populations is PCR-amplified and Sanger sequenced...determined by comparing the edited trace to the control trace. In the ICE algorithm, potential editing ...summary table. You can also use your browser’s Control+F or Command+F functionality to search for a particular...disagreement between the non-edited wild type (control) and the edited sample in the region around the...difference. Traces The Traces tab shows the edited and control, non-edited Sanger traces in the region around ...

  8. Viral Production at Addgene

    Type
    Blog Post
    ...subunit (RPP30) as a control for normalization purposes are being used. Quality Control Mycoplasma Our 293T...virus preps are titered and subjected to quality control by Addgene before being distributed to customers...production protocols, titering methods, and quality control are described below. AAV Production AAV distributed...probes targeting the ITR elements and an internal control of known titer (protocol modified from Lock et ... April 2016) also performed by ddPCR.  Quality Control Addgene ensures high quality viral vectors by optimizing...production protocols and performing rigorous quality control (QC). The specific QC experiments performed varies...

  9. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...unknown sample. To do this, you serially dilute a control sample of known concentration and measure a specific...example, a BCA assay uses a serial dilution of a control protein, incubated alongside an unknown sample ... a standard curve by serially diluting a known control sample, called a standard. The ideal standard curve...curve accordingly.    Figure 1: A 1 mg/mL control is diluted in a series ranging from 1:2 to 1:32... for each run. To validate, include a positive control of known concentration in the experiment and use...  Include both a standard curve and a positive control in every run.  When setting up your dilution series... ELISAs The Four ELISAs and When to Use Them A Control for All Seasons More resources on addgene.org Addgene's...

  10. AAV Viral Preps

    Type
    Collection
    .... Addgene’s comprehensive high-quality quality control process includes titering, purity assessment, sterility... Sensors, Glutamate Sensors, Voltage Reporters Controls Fluorescent Proteins, Spagehetti Monster, Brainbow...Serotype Serotype Testing Samples Small samples of control AAV vectors for trial purposes in serotypes AAV1...

  11. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...will continue to add more controls, and have recently released these controls: pAAV-hSyn-DIO-EGFP (50457...sensors Optogenetics Recombinases And more! Control AAV For many AAV experiments, we recommend validating...entire collection! We are expanding our entire control AAV inventory to give scientists access to a wide... Email us or check our website to access them! Controls pAAV-hSyn-hChR2(H134R)-EYFP (26973-AAVrg) pAAV-hSyn-hChR2...

  12. Bacterial Expression Systems

    Type
    Collection
    ...visualization, study of protein interactions, controlled gene expression, reporter plasmids and genome...Purification Protein Visualization Protein Interactions Controlled Expression Reporter Plasmids Genome Engineering...Studying Protein–Protein Interactions Return to top Controlled Expression It isn’t always advantageous to constitutively...stationary phase. In these cases, you may want to control protein levels or to turn on expression only at...contains plasmids whose expression levels can be controlled by a variety of small molecules, light, and temperature...C) Escherichia coli Yingfeng An Other Addgene Controlled Expression Resources Check out our Tetracycline...Addgene #1000000137) allows you to independently control the expression of up to twelve genes using small-molecule...

  13. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    ...Addgene - we had used Sanger sequencing for quality control for over ten years. Now, thanks to a generous donation...The first step we take when performing quality control of newly deposited plasmids is to align the NGS... by the depositing lab, we call these “quality control (QC) issues.” We then ask the depositing laboratory...

  15. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    ... No spatiotemporal control. Many PPI detection methods lack spatiotemporal control or an “on/off switch... by creating Split-BioID, a spatiotemporally controllable version of the proximity-dependent biotinylation...method Enter Split-BioID, a spatiotemporally controllable version of the BioID protocol. The critical ...interaction partners if they are enriched relative to controls using Split-BioID-GFP and BioID run on six unrelated...methods, including BioID, is its spatiotemporal controlled labeling of interacting proteins; nearby proteins...

  16. Luciferase Plasmid Collection

    Type
    Collection
    ...luciferase gene fusions. Renilla luciferase under the control of a CMV promoter is present for normalization ...Vector Firefly Vector for investigating regions controlling transcription Debrya Groskreutz 18760 MSCV IRES...RapidResponse™ Renilla Vector for investigating regions controlling transcription Pete Stecha 212933 pGL4.82(hRluc...Puro) Renilla Vector for investigating regions controlling transcription Pete Stecha 106292 pLS-SV40-mP-...fusions in plants. Renilla luciferase under the control of a CMV promoter is present for normalization....luciferase. Firefly luciferase expression under the control of a HSV TK promoter for normalization. Modified..., c-Myc, p53, and MAPK/JNK plus a constitutive control. Koen Venken 133315 pMKV060 Firefly AtUbi10 BeYDV...

  17. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type
    Blog Post
    ...systematically study gene function with temporal control and cell-type specificity! The Gal4/UAS system...you mate your fly line containing YFG under the control of the UAS with a fly line expressing Gal4 in your...promoter each time a new hypothesis is tested. Controlling multiple genes with Gal4/UAS system One can also...to drive expression of multiple genes under the control of UAS enhancer sequences. For example, if you ...them individually or simultaneously. Do note for control purposes that there are dosage effects. For example...

  18. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...same correlation, but it wouldn’t be causal. To control for a number of differences between those papers...methodologies, or qualities of research our adjustment controls for them.   So, for example, in 2010, 10 percent...are lots of other factors that could still be controlled for. As an example, plasmid-related areas of ...see such a big difference in citations after controlling for journal. And so, while our quantitative analysis...research tools, computing performance, and randomized control trials in innovation. Visit his website at: http...

  19. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...possible, include positive and negative controls. Positive controls typically include cell lines or tissues...validated applications, pay close attention to the controls listed on the vendor’s website. Typically, the...levels (not overexpression systems) while negative controls are those that do not naturally express the protein...note, however, that you must include a loading control when assessing protein abundance or comparing samples... samples across lanes.  For a typical loading control the membrane is probed with an antibody that detects...transfer causes inconsistent levels of the loading control. In these cases, the western should be repeated...care to include the proper positive and negative controls, choose antibodies that are validated for your...
Showing: 61 - 80 of 494 results