We narrowed to 495 results for: control

...non-standard), expressing Rep2 and PHP.eB Cap controlled by tTA-TRE amplification system Viviana Gradinaru...(non-standard), expressing Rep2 and PHP.S Cap controlled by tTA-TRE amplification system Viviana Gradinaru...non-standard), expressing Rep2 and PHP.V1 Cap controlled by tTA-TRE amplification system Viviana Gradinaru...plasmid (non-standard), expressing Rep2 and B10 Cap controlled by tTA-TRE amplification system Viviana Gradinaru...plasmid (non-standard), expressing Rep2 and B22 Cap controlled by tTA-TRE amplification system Viviana Gradinaru...non-standard), expressing Rep2 and MaCPNS1 Cap controlled by tTA-TRE amplification system Viviana Gradinaru...non-standard), expressing Rep2 and MaCPNS2 Cap controlled by tTA-TRE amplification system Viviana Gradinaru...

...always include proper controls like uncut DNA and the proper ladder. These controls help you properly visualize...sequencing the chromatogram is your visual control. And, like all controls, missing out is a big mistake. Chromatograms...

...protein. With this control set, you should see your target protein IP’d in the positive control sample but not...permanently altering the structure of the protein.  Controls As with any experiment, it is critical to include...include proper positive and negative controls for an IP. When possible, perform the IP in parallel with ...interest and a sample that does not. Common negative controls include knockout cell lines or tissues that do... not in the negative control, confirming that the capture antibody is functioning as expected. In order...perform parallel IPs with beads only and an isotype control antibody. Alternative applications Oftentimes, ...

...subunit (RPP30) as a control for normalization purposes are being used. Quality Control Mycoplasma Our 293T...virus preps are titered and subjected to quality control by Addgene before being distributed to customers...production protocols, titering methods, and quality control are described below. AAV Production AAV distributed...probes targeting the ITR elements and an internal control of known titer (protocol modified from Lock et ... April 2016) also performed by ddPCR.  Quality Control Addgene ensures high quality viral vectors by optimizing...production protocols and performing rigorous quality control (QC). The specific QC experiments performed varies...

...derived from the control trace file. On the plot, the green (edited sample) and orange (control sample) lines...cells, genomic DNA from both edited and unedited (control) populations is PCR-amplified and Sanger sequenced...determined by comparing the edited trace to the control trace. In the ICE algorithm, potential editing ...summary table. You can also use your browser’s Control+F or Command+F functionality to search for a particular...disagreement between the non-edited wild type (control) and the edited sample in the region around the...difference. Traces The Traces tab shows the edited and control, non-edited Sanger traces in the region around ...

...unknown sample. To do this, you serially dilute a control sample of known concentration and measure a specific...example, a BCA assay uses a serial dilution of a control protein, incubated alongside an unknown sample ... a standard curve by serially diluting a known control sample, called a standard. The ideal standard curve...curve accordingly.    Figure 1: A 1 mg/mL control is diluted in a series ranging from 1:2 to 1:32... for each run. To validate, include a positive control of known concentration in the experiment and use...  Include both a standard curve and a positive control in every run.  When setting up your dilution series... ELISAs The Four ELISAs and When to Use Them A Control for All Seasons More resources on addgene.org Addgene's...

...systems, how to use them to modify the genome or control gene expression, and find plasmids for your experiments...engineering tools used to precisely manipulate DNA and control the expression of specific genes in cells or organisms...recombinases can be used to modify the genome or control gene expression or skip ahead to browse highlighted...Summary of Cre, Dre, and Flp Recognition Sites. Controlling Recombinase Expression By expressing the enzyme...promoters or inducible systems, you can precisely control and optimize expression of your gene of interest... and C-terminal fragments and placed under the control of different promoters. Expression of both fragments...experimental system, enabling multi-layered genetic control. Recombinase-mediated cassette exchange (RMCE):...

...Production and Quality Control Production Our production process and quality control, with examples of quality...production, purification, and comprehensive quality control. In-Stock Preps 1,000+ preps ready to ship immediately...quality control data. Help Center Articles Additional details about viral vector production at Addgene...

...will continue to add more controls, and have recently released these controls: pAAV-hSyn-DIO-EGFP (50457...sensors Optogenetics Recombinases And more! Control AAV For many AAV experiments, we recommend validating...entire collection! We are expanding our entire control AAV inventory to give scientists access to a wide... Email us or check our website to access them! Controls pAAV-hSyn-hChR2(H134R)-EYFP (26973-AAVrg) pAAV-hSyn-hChR2...

...Addgene - we had used Sanger sequencing for quality control for over ten years. Now, thanks to a generous donation...The first step we take when performing quality control of newly deposited plasmids is to align the NGS... by the depositing lab, we call these “quality control (QC) issues.” We then ask the depositing laboratory...

... just label cells. If GFP could be co-opted to control gene expression, he could then selectively manipulate...

... No spatiotemporal control. Many PPI detection methods lack spatiotemporal control or an “on/off switch... by creating Split-BioID, a spatiotemporally controllable version of the proximity-dependent biotinylation...method Enter Split-BioID, a spatiotemporally controllable version of the BioID protocol. The critical ...interaction partners if they are enriched relative to controls using Split-BioID-GFP and BioID run on six unrelated...methods, including BioID, is its spatiotemporal controlled labeling of interacting proteins; nearby proteins...

...systematically study gene function with temporal control and cell-type specificity! The Gal4/UAS system...you mate your fly line containing YFG under the control of the UAS with a fly line expressing Gal4 in your...promoter each time a new hypothesis is tested. Controlling multiple genes with Gal4/UAS system One can also...to drive expression of multiple genes under the control of UAS enhancer sequences. For example, if you ...them individually or simultaneously. Do note for control purposes that there are dosage effects. For example...

...same correlation, but it wouldn’t be causal. To control for a number of differences between those papers...methodologies, or qualities of research our adjustment controls for them.   So, for example, in 2010, 10 percent...are lots of other factors that could still be controlled for. As an example, plasmid-related areas of ...see such a big difference in citations after controlling for journal. And so, while our quantitative analysis...research tools, computing performance, and randomized control trials in innovation. Visit his website at: http...

...available, we have listed cloning backbones and control plasmids. Donor Acceptor λ Dex QY D λ Aem ε A QY...refer to published reports and include appropriate controls to help interpret your results. See Algar et al...feature plasmids with positive and negative FRET controls or calibration standards consisting of donor and...quantitative two-hybrid FRET Positive and negative controls and cloning backbones for mTurquoise2-mVenus(L68V...towards a quantum yield of 93% Positive and negative controls and cloning backbones for various FRET pairs with...acceptors for mTurquoise2 Positive and negative controls and cloning backbones for red, yellow, and turquoise...fluorescent protein Variety of fusion proteins, positive controls for many FRET pairs Michael Davidson Lab (Unpublished...

...possible, include positive and negative controls. Positive controls typically include cell lines or tissues...validated applications, pay close attention to the controls listed on the vendor’s website. Typically, the...levels (not overexpression systems) while negative controls are those that do not naturally express the protein...note, however, that you must include a loading control when assessing protein abundance or comparing samples... samples across lanes.  For a typical loading control the membrane is probed with an antibody that detects...transfer causes inconsistent levels of the loading control. In these cases, the western should be repeated...care to include the proper positive and negative controls, choose antibodies that are validated for your...

...Their system is based on the AraC protein that controls transcription from the PBAD promoter. “AraC is... of interest, under the pBAD promoter, can be controlled with light without the need of any cloning. ...

...Packaging Service AAV Controls Viral Vector Packaging Service: Control AAV Browse our control AAV that express... select plasmids in the repository. Browse our control AAV collection, including fluorescent proteins,...consider using fewer filtering options. Browse all Control AAV *AAVrg = retrograde serotype, produced with...

...living cells. Since bioelectrical signals are controlled by the opening and closing of ion channel proteins...genetically encoded, light-gated ion translocators to control tumorigenesis." Oncotarget 7.15 (2016): 19575. ...signaling networks: exploiting voltage gradients for control of growth and form." Annual review of biomedical... neural and gap junction protein-mediated cues control polarity during planarian regeneration." Developmental...Vaibhav P., et al. "Transmembrane voltage potential controls embryonic eye patterning in Xenopus laevis." Development...

...input signal that both positively and negatively controls the output. In this case, plasmid copy number .... placed a trans-acting RepA protein under the control of IPTG. Because RepA is a plasmid replication ...Segall-Shapiro et al. placed the genes under the control of a constitutive promoter and then moved them ...decreased. But when the genes were placed under the control of a stabilized promoter, deoxychromoviridans titers...the joys of a p-value < 0.05, having such fine control of gene expression is useful for many metabolic...