We narrowed to 142 results for: endog
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TypeBlog Post...that do not express your protein of interest endogenously and transfect them with a plasmid encoding your...ultimately planning to use your antibody to detect endogenous levels of the target in other cells or tissues...
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Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post...Additional Resources on the Addgene Blog Plasmids for endogenous gene tagging in human cells Plasmids 101: Protein...Protein Tags Fluorescent tagging of endogenous genes with SapTrap Resources on Addgene.org Browse CRISPR... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post...a molecular species with high affinity for an endogenous molecular target, meaning the dye will be attached...results in direct binding between the dye and the endogenous target, and thus no genetically encoded tag is... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post...system described above, except that they use an endogenous nuclease system to cleave the gRNAs. gRNAs are...CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system.” Proceedings of the National... -
Zhang Lab CRISPR Page
TypeCollection...complex for the transcriptional activation of endogenous genes. It consists of three components: A nucleolytically...sets of 3 vectors each available for mammalian endogenous gene activation using SAM: Addgene plasmids #61422... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system Plant Utilizes the tRNA... promoters. Bullock pCFD5 Drosophila Utilizes endogenous tRNA processing machinery to excise multiple ... -
The 12 Days of CRISPR: 2021
TypeBlog Post... day of CRISPR, Addgene gave to me: Selective Endogenous eNcapsidation for cellular Delivery (SEND). On... -
Inntags: Innovative Protein Epitope Tagging
TypeBlog Post...Plasmids 101 Series. Get the Inntag Plasmids Tag endogenous proteins with CRISPR Fluorescently Tag Your Protein... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog Post...Controlling Stimulation ExogenousEasily controlled EndogenousNot easily controlled Unique advantages Mapping... -
Sensing Neuronal Dopamine
TypeBlog Post...to gather the following types of data: Report endogenous DA release from a single dopaminergic fiber in... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog Post...organism’s genome (see figure) and to disrupt endogenous genes at the site of insertion. In both of these... -
Antibodies 101: Epitope Tags
TypeBlog Post...separation of detection of a mutant protein vs. endogenous WT levels of the same protein when both are expressed... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog Post...been to either systematically delete/overexpress endogenous genes or to introduce heterologous genes, but... -
iPSC Neurodegenerative Disease Initiative Plasmid Collection
TypeCollection...plasmids can be used to create cell lines with endogenously-tagged gene variants or to differentiate human... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post...allows for whole organism imaging. They created an endogenous ApoB-NanoLuc reporter by inserting the NanoLuc... -
DAP arrays for multiplex gene editing
TypeBlog Post...expression, the individual gRNAs are released by endogenous tRNA processing machineries, specifically, RNaseZ... -
K. phaffii: Rising to the Occasion
TypeBlog Post...cinch. This is because it doesn’t secrete its own endogenous proteins, making the recombinant protein its ... -
Hot Plasmids - January 2023
TypeBlog Post...also demonstrated the ability to be used as an endogenous RNA imaging tool. Fig. 1: Csm, shRNA... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...useful for therapeutic applications in vivo where endogenous anti-AAV antibodies often neutralize the therapeutic... -
Hot Plasmids - February 2022
TypeBlog Post...levels of gene activation for both reporter and endogenous genes. It is highly specific in mammalian cells...