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We narrowed to 369 results for: epor

Showing: 61 - 80 of 369 results

  1. Making Career Development an Integral Part of the Science Training System

    Type
    Blog Post
    ...Career Development; a Stakeholder Workshop Report.” The report enabled the development of priorities for...Development Hub Workshop Attendees and their 2021 report. Image from pd|hub.   To begin to tackle these...the findings were captured in this just released report “Enhancing Dissemination of Evidence-Based Models...programs.  Please join us for the pd|hub Workshop Report Public Release Event scheduled for March 4th from...2:30-4:00 pm ET.  You can learn more about the report's recommendations and join with us to discuss and...

  2. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    ... the TIMER growth rate bioreporter. During protein maturation, the bioreporter spontaneously changes from..., Bernach plans to use a suite of fluorescent reporters. Bacteria containing unstable fluorescent proteins...artificial leaf, Bernach will use the CUSPER bioreporter such that fluorescent proteins expression is ...colonizer, labelled with a constitutively fluorescent reporter can also be used to track how one population responds...using TIMER and an unstable fluorescent protein reporter under the control of promoters that control cell...

  3. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...scientific communities. The ARRIVE guidelines for the reporting of laboratory animals during in vivo research ...research and the CARE guidelines for case reports are two sets of guidelines frequently used by editors. Providing...s name and affiliations, and the entire referee report are permanently attached to the published article...and readers are able to comment on the referee reports. If the reviewers suggest that the manuscript requires...article, while the first version and the referee reports remain available online. Open-peer reviewing is...and effort) and the scientific community, as the reports can be very informative as to how a paper is received...

  4. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...in genome target sequence into EGFP reporter. Target DNA reporter system: Can evaluate component delivery...mutation? Do you want to create a fusion with a reporter protein such as GFP? All of these outcomes can...promoter is typically used for gRNA. May contain reporter gene (e.g. GFP) to identify and enrich positive...vector or separate transfer vectors. May contain reporter gene (e.g. GFP) to identify and enrich positive...published as CRISPR testing tools: Traffic Light Reporter System: Can evaluate lentiviral component delivery...

  5. Technical Design of a Western Blot

    Type
    Blog Post
    ...primary antibody required. Reporters There are two main classes of reporters: chemiluminescence enzymes...you need a specific conjugate, and changing your reporter molecule requires either re-conjugating or re-...-purchasing your primary antibody with the new reporter. Finally, each new conjugate will have to be tested...film is only compatible with chemiluminescence reporters and requires access to a darkroom and a developer... time; higher background Chemiluminescence reporter Smaller dynamic range; no multiplexing Protein-based... time; higher background Chemiluminescence reporter Smaller dynamic range; no multiplexing Wet...

  6. Deep Dive: qPCR

    Type
    Blog Post
    ...step. Scorpion chemistry in particular has been reported to work well in the Lightcycler while being relatively...Yes Agarose gel Sunrise primers   Low Reporter and quencher on hairpin structure included in ...much as sixfold variation in expression levels reported (Biassoni, 2014), so you’ll want to ensure that...conceptualize. Just remember to switch them back before reporting the data!   Statistical analysis  Getting...frown upon statistical laxness in all its forms. Reporting your data  Once you’ve done the work to produce...(Bustin, 2009) to ensure you’re recording and reporting the right information so others can reproduce ...

  7. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ... targeting astroglia. It has additionally been reported to have tropism for liver, vascular endothelial... muscle inflammation and degeneration has been reported (Muraine et al., 2020). AAV8 AAV8 has high transduction... the CNS (Han et al., 2023), and has also been reported to have tropism for liver and kidney cells (Issa...Use Addgene’s AAV Data Hub to find experimental reports on commonly used vector cargo designs and administration...cells utilizing various AAV serotypes. Scientific Reports, 15(1), 20927. https://doi.org/10.1038/s41598-025...

  8. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    ... to all articles, Anurag Acharya and colleagues report, the percentage of citations to articles in non-elite...publications. (As an interesting aside, Nature recently reported that the most cited work in history is a 1951 ... the best way to measure impact? As that Nature report also noted, papers detailing Nobel Prize-winning... for the impact of research" (2013) Scientific Reports 3, Article number: 1649 doi:10.1038/srep01649 ...

  9. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...detects. This change then generates a reporter signal. Reporter signals may be electrochemical or light-based...light-based, with luminescent and fluorescent reporters being especially popular. We’ll give you an introduction...glutamate sensor pCMV(MinDis).iGluSnFR. Picking your reporter Don’t forget about the other end of the biosensor...

  10. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...behaving mice to gather the following types of data: Report time-course and concentration of released DA in...optogenetically activated and inhibited neurons in live mice Report modulation of DA signaling during learning and ...moving mice to gather the following types of data: Report endogenous DA release from a single dopaminergic...looming stimulus in vivo in transgenic zebrafish Report DA release in live mice over the course of Pavlovian...

  11. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...Lab at MIT developed plasmid based systems that report the order in which specific chemical inputs are...recombinase-based state machines could potentially be used to report upon and/or engineer “temporally ordered biochemical...derived from Zika virus and, upon binding, produce reporter proteins. These toehold switches can be used to...viral replication and transcription as well as reporter genes. To learn how you can use minigenomes in...

  12. RUBY-Red Siliques

    Type
    Blog Post
    ...challenging and requires specialized equipment. Reporters for studying gene expression have similar drawbacks...added to the palette. RUBY is a bright red pigment reporter whose expresion is visible to the naked eye in... enzymes and a spontaneous step. (B) RUBY is a reporter consisting of these three enzymes expressed under...References  He, Y., Zhang, T., Sun, H. et al. A reporter for noninvasively monitoring gene expression and...

  13. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ..., the MIT Technology Review dropped a bombshell report. A scientist working in China was using CRISPR/...While the Nuffield Council has recently published a report on the social and ethical issues of genome editing...question of “how do we move forward” is the June 2012 report on the ethics of “Novel techniques for the prevention...diseases. The key success of the Nuffield Council’s report is that when it became clear that this assistive...

  15. Hot Plasmids - May 2022

    Type
    Blog Post
    ... a cleavage free manner. The dCas9-SSAP system reports an efficiency of up to 20%, comparable to commonly... For a one kilobase insertion, the researchers reported an editing error rate of less than 0.3%, both ...using mStrawberry, localization peptides, and reporters that drive the expression of the fluorescent protein...response element (TRE) or kinase translocation reporters (KTRs). Find visual barcode plasmids here! Kaufman...

  16. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...GCaMP6 family of sensors are commonly used calcium reporters with different kinetic parameters. There are three...also available. Detection methods Many calcium reporters use a single fluorescent protein (FP), requiring...unwanted variables. Twitch is a FRET-based calcium reporter derived from troponin C. It is engineered with...for in vivo studies) There are several calcium reporters specially designed for the aims and requirements...

  17. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...mild symptoms. This changed in January with the report of an outbreak of the virus in Brazil that was ...-ready format when the Zika virus outbreak was reported, and we felt that, given the critical need for...such a short timescale. Our article ultimately reported the development of a rapid design and prototyping...Find the Zika Sensor Constructs at Addgene As we report, we have embedded this next generation of toehold...

  18. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ... available with T-Sapphire, EGFP, or mScarlet reporters, so you can choose a color compatible with the...and AAV11 tropism and applications. A) AAV6 is reported to have broad tissue tropism, including skeletal...hematopoietic cells, and immune cells. B) AAV11 is reported to target skeletal muscle, kidney, spleen, lung...the Cre recombinase payload activates a floxed reporter gene (genetically encoded or delivered separately...

  19. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    ...Fluorescent Protein (GFP/EGFP) for a transcriptional reporter or as a general cytoplasmic label of cells, there's...FP dimerization. Equally problematic, several reportedly monomeric FPs are not actually monomeric, at ...Incorporation into a FRET biosensor A FRET biosensor is a reporter that undergoes a conformational change to bring...help you make better fluorescent fusion protein reporters for your studies and greatly lower your chances...

  20. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...are removed and replaced by a single (non-viral) reporter gene (e.g. firefly luciferase or eGFP) flanked...necessary to direct minigenome replication and reporter gene transcription by the EBOV polymerase (reviewed...the minigenome as a template to transcribe the reporter gene (e.g. firefly luciferase). The polymerase...start signal and initiates transcription of the reporter gene. The polymerase will elongate the mRNA until...
Showing: 61 - 80 of 369 results