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We narrowed to 89 results for: grna cloning vector

Showing: 61 - 80 of 89 results

  1. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...Addgene website in December 2006, the pLKO.1-TRC cloning vector (deposited by David Root of the Broad Institute...protocol listed under “Supplemental Document”: gRNA_Cloning Vector (deposited by George Church’s lab). Addgene...Recently I learned that Addgene’s pLKO.1 cloning protocol is viewed around 3,000 times each month. I ...has also cataloged a variety of useful molecular cloning protocols, including making LB agar plates, restriction...labs, and now Addgene. I just began uploading our cloning protocols to the site and will keep you updated...

  2. CRISPR Plasmids - Bacteria

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila....1016/j.bbrc.2013.08.013. Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...

  3. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...guide RNA delivery does however rely on cloning into a vector or in vitro synthesis, which could be less...transfect/inject cells with in vitro preassembled Cas9/gRNA complexes or with guide RNA- and Cas9-expression...

  4. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Publication Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing ...find a gRNA vector based on expression system, promoter, the type of gRNA (e.g., pegRNA, epegRNA, nicking...using a prime editing guide RNA (pegRNA). As with a typical gRNA, the pegRNA is designed with a spacer that...nicking sgRNA), cloning enzyme, selectable marker, and whether the plasmid contains Cas9. ID Plasmid Expression...Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI 132777 pU6-pegRNA-GG-acceptor Mammalian...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Prime editing is ...

  5. CRISPR Plasmids - Plants

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...pyogenes Bar Chen 53063 pU3-gRNA OsU3 AarI none S. pyogenes Gao 53061 pZmU3-gRNA maize U3 none S. pyogenes...pyogenes Gao 53062 pU6-gRNA wheat U6 BbsI none S. pyogenes Gao 59188 pBlu/gRNA Arabidopsis U6 BbsI none S. ...

  6. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...Replication What is a Plasmid? How to Design Your gRNA for CRISPR Editing The Promoter Region - Let's Go...virus, I learned from Addgene,” or “I learned to clone from the Addgene website.” We delight in seeing ... CRISPRTwenty Years of Sharing: Addgene's Viral Vector Service...

  7. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Publication Empty gRNA Expression Vectors You can use the tables on Addgene's Empty gRNA Vectors page to search...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...such as selectable marker or cloning method. To isolate mammalian vectors, simply type "Mammalian" into...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...

  8. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ....1 - TRC cloning vector - Lentiviral shRNA expression pLJM1-EGFP - 3rd gen lentiviral vector for EGFP ... pLX304 - 3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-blast - 3rd ...Independent Cloning (LIC) pAG CMV Empty Puro - Empty mammalian expression plasmid for cloning untagged proteins... Protein expression vector with C-terminal mTagBFP2 tag pNIC-GST-TEV-GG - Cloning of target gene with ...pFastBac Dual LIC - Vectors from the Gradia lab pFB-LIC-Bse - Insect expression vector from Gileadi lab ...expression vector with N- or C-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF...

  9. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ...Practical Guide: Using pK-TnpB1 and pK-TnpB2 Vectors The vectors pK-TnpB1 and pK-TnpB2 are designed for plant...selection. The process for cloning the 20-nt guide RNA is identical for both vectors. Here, we provide a step-by-step...the digested vector. Step 4: Ligation Ligate the annealed oligos into the digested vector using your ...efficient delivery into cells, particularly via viral vectors. While viral delivery is a highly effective method...genome editors to date. We have developed multiple vector versions for TnpB-mediated genome editing, and ...delivery of editing reagents into cells. Viral vectors, because of their natural ability to infect cells...approval and public acceptance. Recently, viral vectors have been successfully used to generate gene-edited...

  10. Zhang Lab CRISPR Page

    Type
    Collection
    ...is indicated below) can be cloned scarlessly into the vector before the sgRNA scaffold. The oligos are ... in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9...of annealed oligos can be cloned scarlessly into the vector before the sgRNA scaffold. The oligos are ...Protocols 2013). For cloning information, please view: Zhang Lab General Cloning Protocol 237.2 KB SpCas9...lentiviral vector encoding dCAS9-VP64 with 2A Blast resistance marker 61426 : lentiviral vector encoding...61408 : Targeting vector for the mouse Rosa26 locus; Used to make Cas9 knockin mouse Vectors for cancer modeling...for cloning new targets are described here. #60224 - AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1...

  11. Microbiology Resources

    Type
    Collection
    ...Broad-Range Rainbow Vectors - Barbier Lab Cloning and Expression Bacteria and yeast expression vectors : FX cloning...Parts Kit : Molecular cloning for E. coli - Densmore Lab EcoFlex MoClo : Modular cloning for applications ... and Dutzler Labs EasyClone 2.0 Yeast Toolkit - Borodina Lab Golden GATEway cloning system - Wittbrodt...Microbiology : Sets of plasmids for fluorescence, cloning and expression, and synthetic biology. CRISPR Resources...sets of plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied ...Yarrowia lipolytica Golden Gate tool kit : Integrative vector assembly of up to three transcription units in ...lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors for expression, markers, fusion proteins, etc -...

  12. Hot Plasmids February 2024

    Type
    Blog Post
    ...developed an engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene...N.C., Goeden, N. et al. Adeno-associated viral vectors for functional intravenous gene transfer throughout...region VIII of AAV9-X1.1 to that of AAV1. This viral vector has brain endothelial cell-specific tropism, with... by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing. In a ...use in western blots, though the original parent clone (M2) has been widely used in a variety of applications...endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded editing of 80–90% in human cell...limited (likely due to its long, negatively charged sgRNA), RNP delivery was much better with Cas12, which...

  13. Lentivirus Plasmids

    Type
    Collection
    ...plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table and search "lenti....1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid; includes a stuffer for easy cloning Root 14748...Kasparov 1732 pCPRDEnv 2nd Packaging part of the FELIX vector system, expresses Gag-Pol and Rev Nolan 35617 pCAG-Eco...shRNA. See article for detailed information about cloning & additional plasmids. Aebischer and Trono 11795...cassette (promoter and gene of interest) can be cloned into the plasmid. Nolan 12257 pWPXL 2nd EF-1alpha... the gene of interest, also can express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in...

  14. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...of plasmid vectors and/or rely on cumbersome in vitro synthesis of single guide RNAs (sgRNAs), are time-consuming...is no need to construct plasmid vectors or synthesize/purify IVT sgRNA in the lab. Figure 2: Cas9 RNP... alleles may be warranted (20-30% of all tagged clones were bi-allelic in our hands). Take-home message...genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA. J Biotechnol 241:...complexed with sgRNA. Direct comparison of Cas9 RNPs prepared with an in vitro-transcribed (IVT) sgRNA versus ... the in vitro-transcribed single guide RNA (IVT sgRNA) approach (Dewari et al. 2018 and Figure 2). Not...

  15. CRISPR Guide

    Type
    Collection
    ...come in 1-vector systems, in which Cas9 is included in the gRNA-containing plasmid, or 2-vector systems,... of unique gRNAs. gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high ...sequence is cloned into a gRNA expression plasmid but does NOT include the PAM sequence or the gRNA scaffold... guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short synthetic...commonly referred to as an sgRNA. gRNA scaffold sequence The sequence within the gRNA that is responsible for...amplify a specific sequence of DNA pegRNA P rime E dit gRNA; a specialized gRNA containing an additional primer...Cas enzyme and a gRNA specific to the gene of interest. The genomic target of the gRNA can be any ~20 nucleotide...

  16. Worm Expression Resources

    Type
    Collection
    ...General Tools Fire Lab C. elegans Vector Kit - Andrew Fire Lab. A set of vectors for C. elegans research, including...sets of plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied ...resources include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous tagging...plasmids that streamlines the production of targeting vectors for tag insertion using CRISPR/Cas9 and the selection...editing in C. elegans via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified...including lacZ and GFP fusion vectors. C. elegans optimized fluorophores - Andrew Fire Lab. Available ...

  17. Plasmid Collections

    Type
    Collection
    ... view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology...libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor Collections A modular set of plasmids ...Proteins Protein Expression Synthetic Biology Viral Vectors Communities Find a scientific community that has...

  18. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...design and clone 500-700 bp homology arms for your gene of interest into the FP-SEC vector using Gibson...3xFLAG-tag fusion proteins. The repair template and Cas9-sgRNA plasmid is then injected into the germline of young...

  19. Zebrafish Plasmid Collection

    Type
    Collection
    .... pGCS Vector Kit - Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS),... selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of useful zebrafish CRISPR...found below: UFlip CRISPR targeted integration vectors for cell-type specific conditional gene inactivation...inactivation - Jeffrey Essner Lab GeneWeld Vectors for Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner...mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish...with fluorescent proteins to visualize and track clones of cells over time. Examples of useful tools for...with four promoters, six fluorophores, and empty vectors designed to streamline the production of transgenic...

  20. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...CRISPR/Cas9 Clones for Deletions and Clone Selection For suspension cells, transfer all clones to a single...deletion clones with a proofreading polymerase and clone the amplicons ( e.g., with a PCR cloning kit) into... the guide’s reverse complement for cloning into the pX330 vector using BbsI restriction enzyme ( Table...Gate assembly cloning strategy 3 . Prepare a 50 μl reaction mix: 100 ng circular pX330 vector, 1.0 μl annealed...one plate to allow for clones to grow and use the other plate to screen each clone for deletions. Add an...Validation of Biallelic Deletion Clones In order to characterize obtained clones and validate a successful knockout... knockout, two sgRNA located within exons will enrich even monoallelic deletion clones for loss of function...
Showing: 61 - 80 of 89 results