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We narrowed to 66 results for: ha/1000

Showing: 61 - 66 of 66 results
  1. Pouring LB Agar Plates

    Type
    Protocol
    ...pipettes Ice bucket to hold antibiotic Antibiotic at 1000 x concentration disolved in the appropriate liquid...darken during the autoclave process if your sample has spent at least 10 min at 121 ℃. Use lab tape to label...antibiotic to the molten gel mix, you should create a 1000x stock solution. For example: If you’ll be preparing...
  2. CRISPR Library Amplification

    Type
    Protocol
    ...MaxiPreps (Qiagen HiSpeed Max, Catalog #12663) Tips (1000 µL, 200 µL, 10 µL) Bacti Cell Spreaders (VWR, 60828... evenly with a sterile spreader until all liquid has been absorbed by the agar. This usually takes 1-2... 100 x 100 ÷ 0.1 This number should be at least 1000X greater than number of perturbations in library....least 10 colonies on the most diluted plate for 1000x coverage of a library of 100,000 plasmids. Colonies...
  3. Lentivirus ddPCR Titration

    Type
    Protocol
    ...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette 2–50 µL multichannel pipette...sealer and press the ‘Seal’ button. After the plate has been sealed, proceed to thermocycling. Thermal Cycling...
  4. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...an impure AAV preparation. The 15% iodixanol step has 1M NaCl to destabilize ionic interactions between... and forth a few times to mix the iodixanol that has settled at the bottom of the column. We recommend...bring up the volume with formulation buffer. Use a P1000 to the bottom of the filter and pipette up/down ...
  5. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...antibiotic required for effective over-agar selection has been empirically determined. See selection curve ...without antibiotic High concentration (100 mg/mL, 1000x) carbenicillin stock solution in sterile water (...
  6. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...Yeast Extract and dH 2 O to 400 mL Note: If your lab has pre-mixed LB agar powder, use the suggested amount... dissolved in dH 2 0. Addgene recommends making 1000X stock solutions and storing aliquots at -20 °C. ...media, add 100 μL of a 100 mg/mL ampicillin stock (1000X stock) to 100 mL of LB. Antibiotic Concentrations...
Showing: 61 - 66 of 66 results