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  1. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...Vulnerable Patients. Frontiers in Immunology, 13. https://www.frontiersin.org/articles/10.3389/fimmu.2022.885672Piché...Safety Assessment (pp. 519–542). Academic Press. https://doi.org/10.1016/B978-0-12-417144-2.00027-5van ... Challenges. The oncologist, 21(10), 1260–1268. https://doi.org/10.1634/theoncologist.2016-0061WHO. International...Enhancements. Frontiers in drug discovery, 2, 952326. https://doi.org/10.3389/fddsv.2022.952326Joubert, M. K...Antibody-Based Biotherapeutics. PloS one, 11(8), e0159328. https://doi.org/10.1371/journal.pone.0159328Groell, F....Pharmazeutische Verfahrenstechnik e.V, 130, 128–142. https://doi.org/10.1016/j.ejpb.2018.06.008Lu, RM., Hwang...treatment of diseases. J Biomed Sci 27, 1 (2020). https://doi.org/10.1186/s12929-019-0592-z  Resources Antibodies...

  2. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ... Function Phenotypes. Plant Biology 7:251–257 . https://doi.org/10.1055/s-2005-837597 Li J-F, Chung HS... Silencing in Plants. Plant Cell 25:1507–1522 . https://doi.org/10.1105/tpc.113.112235 Morgens DW, Deans...for essential genes. Nat Biotechnol 34:634–636 . https://doi.org/10.1038/nbt.3567 Nakaoka Y, Miki T, Fujioka...Microtubule Generation. Plant Cell 24:1478–1493 . https://doi.org/10.1105/tpc.112.098509 Smits AH, Ziebell...in CRISPR knock outs. Nat Methods 16:1087–1093 . https://doi.org/10.1038/s41592-019-0614-5 Sztal TE, Stainier...genetic robustness. Development 147:dev186452 . https://doi.org/10.1242/dev.186452 Wilson RC, Doudna JA...RNA Interference. Annu Rev Biophys 42:217–239 . https://doi.org/10.1146/annurev-biophys-083012-130404 ...

  3. Summer SciComm: Preprints

    Type
    Blog Post
    ...Resources References ASAPbio, Preprint Resource Center: https://asapbio.org/preprint-info ASAPbio, Preprints and... and Rapid Communication of COVID-19 research: https://asapbio.org/preprints-and-covid-19 Ettinger CL,...preprinting for early-career researchers. Biol Open. https://doi.org/10.1242/bio.059310 Fraser N, Mayr P, ...preprints: a survey of bioRxiv authors. bioRxiv. https://doi.org/10.1101/2021.09.07.459259 Sarabipour S...early career researcher perspective. PLoS Biol. https://doi.org/10.1371/journal.pbio.3000151 Sever R, ...bioRxiv: the preprint server for biology. bioRxiv. https://doi.org/10.1101/833400  Stern BM, O’Shea EK (2019...scientific publishing in the life sciences. PLoS Biol. https://doi.org/10.1371/journal.pbio.3000116 More resources...

  4. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...by an anti-CRISPR protein. Nature 546:436–439 . https://doi.org/10.1038/nature22377 Harrington LB, Doxzen...Inhibitor of CRISPR-Cas9. Cell 170:1224-1233.e15 . https://doi.org/10.1016/j.cell.2017.07.037 Li C, Psatha...Therapy - Methods & Clinical Development 9:390–401 . https://doi.org/10.1016/j.omtm.2018.04.008 Marino ND, ...CRISPR-Cas technologies. Nat Methods 17:471–479 . https://doi.org/10.1038/s41592-020-0771-6 Mayo-Muñoz D...islandicus Rod-Shaped Virus 2. Viruses 10:695 . https://doi.org/10.3390/v10120695 Pawluk A, Amrani N, ...Off-Switches for CRISPR-Cas9. Cell 167:1829-1838.e9 . https://doi.org/10.1016/j.cell.2016.11.017 Shin J, Jiang... an anti-CRISPR DNA mimic. Sci Adv 3:e1701620 . https://doi.org/10.1126/sciadv.1701620 Additional Resources...

  5. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...Designer Drugs. ACS Chemical Neuroscience 6:476–484 . https://doi.org/10.1021/cn500325v Goutaudier, R., Coizet...Specific Ligands. Eneuro, 6(5), ENEURO.0171-19.2019. https://doi.org/10.1523/ENEURO.0171-19.2019Jendryka M,...DREADD-based chemogenetics in mice. Scientific Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus C, Lee... clinical applications. Science 6436: eaav5282. https://science.sciencemag.org/content/364/6436/eaav5282...transgenic mice. Nature Biotechnology 17:165–169 . https://doi.org/10.1038/6165 Roth BL (2016) DREADDs for...for Neuroscientists. Neuron 89:683–694 . https://doi.org/10.1016/j.neuron.2016.01.040 Saloman, J. L., ...The Journal of Neuroscience, 36(42), 10769–10781. https://doi.org/10.1523/JNEUROSCI.3480-15.2016 Weston ...

  6. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...CRISPR-Cas9 Genome Editing. Circ Res 115:488–492 . https://doi.org/10.1161/circresaha.115.304351 Holkers ...engineered nucleases. Nat Methods 11:1051–1057 . https://doi.org/10.1038/nmeth.3075 Lukashev AN, Zamyatnin... perspectives. Biochemistry Moscow 81:700–708 . https://doi.org/10.1134/s0006297916070063 Maddalo D, Manchado...with the CRISPR/Cas9 system. Nature 516:423–427 . https://doi.org/10.1038/nature13902 Maggio I, Holkers ...in a diverse array of human cells. Sci Rep 4: . https://doi.org/10.1038/srep05105 Stephens CJ, Lauron ...Cas9. Journal of Controlled Release 298:128–141 . https://doi.org/10.1016/j.jconrel.2019.02.009 Wang D, ...Immune Responses. Human Gene Therapy 26:432–442 . https://doi.org/10.1089/hum.2015.087 Additional resources...

  7. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...Cassiopea xamachana. Marine Biology 94:423–430 . https://doi.org/10.1007/bf00428249 Chan MCY, Karasawa ...Journal of Biological Chemistry 281:37813–37819 . https://doi.org/10.1074/jbc.m606921200 Dove SG, Takabayashi...Acroporid Corals. The Biological Bulletin 189:288–297 . https://doi.org/10.2307/1542146 Lazarus, J. E., Warr, ...Applied and Environmental Microbiology, 85(16). https://doi.org/10.1128/aem.00990-19 Liljeruhm, J., Funk...biology. Journal of Biological Engineering, 12(1). https://doi.org/10.1186/s13036-018-0100-0 Murakoshi, H...expression in living cells. Scientific Reports, 9(1). https://doi.org/10.1038/s41598-019-48604-4 Salih A, Larkum...corals are photoprotective. Nature 408:850–853 . https://doi.org/10.1038/35048564...

  8. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...unwinding steps. Molecular Cell, 70(3), 385-394.e3. https://doi.org/10.1016/j.molcel.2018.03.031 Xiao, Y.,...degradation by Cascade and Cas3. Science, 361(6397). https://doi.org/10.1126/science.aat0839 Yoshimi, K., &...CRISPR system. Gene and Genome Editing, 3–4, 100013. https://doi.org/10.1016/j.ggedit.2022.100013 O’Brien, ...CRISPR Cascade. Molecular Cell, 83(5), 746-758.e5. https://doi.org/10.1016/j.molcel.2023.01.024 Morisaka,...editing in human cells. Nature Communications, 10(1). https://doi.org/10.1038/s41467-019-13226-x Cameron, P....cells. Nature Biotechnology, 37(12), 1471–1477. https://doi.org/10.1038/s41587-019-0310-0 Makarova, K..... Nature Reviews Microbiology, 13(11), 722–736. https://doi.org/10.1038/nrmicro3569 Additional resources...

  9. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...Channels. Biophysical Journal, 116(3), 454–468. https://doi.org/10.1016/j.bpj.2019.01.003.  Frank Hayward...the brightness of fluorescent proteins. Zenodo. https://doi.org/10.5281/zenodo.11406498. andrewgyork.github.io...magnetoreception. Protein Science, 30(8), 1521–1534. https://doi.org/10.1002/pro.4124.  Kattnig, D. R., Evans...magnetoreception. Nature Chemistry, 8(4), 384–391. https://doi.org/10.1038/nchem.2447.  Lee, H., Yang, N....Pair Reaction. Nano Letters, 11(12), 5367–5372. https://doi.org/10.1021/nl202950h.  Matsuoka, R., Kimura...American Chemical Society, 145(25), 13615–13622. https://doi.org/10.1021/jacs.3c01076.  Wheeler, M. A.,...nervous system. Nature Neuroscience, 19(5), 756–761. https://doi.org/10.1038/nn.4265.  Additional resources...

  10. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...CRISPR screens. bioRxiv 2024.03.17.585235; doi: https://doi.org/10.1101/2024.03.17.585235.   Improved...small RNA-binding protein. Nature. 2024 Apr 3. doi: https://doi.org/10.1038/s41586-024-07259-6. PMID: 38570691...specific monoclonal antibody. Virology. 558, 28–37. https://doi.org/10.1016/j.virol.2021.01.003. PMID: 33714753...JaneliaFluor dye emitting at 525 nm. Image reused from https://www.janelia.org/open-science/voltron-and-positron... al. (2023) Neuron, 111(10), 1547–1563.e9. doi: https://doi.org/10.1016/j.neuron.2023.03.009. PMID: 37015225...Adeno-Associated Virus. bioRxiv 2023.12.12.571321; doi: https://doi.org/10.1101/2023.12.12.571321.  Revolutionizing...editing and gene therapy. Nat Commun., 14(1), 6578. https://doi.org/10.1038/s41467-023-42386-0. PMID: 37852949...

  11. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ... embryos. Nature Biotechnology, 42(4), 638–650. https://doi.org/10.1038/s41587-023-01821-9 He, Y., Zhou...base editing. Molecular Cell, 84(7), 1257-1270.e6. https://doi.org/10.1016/j.molcel.2024.01.021 Tong, H.,...glycosylase. National Science Review, 10(8), nwad143. https://doi.org/10.1093/nsr/nwad143 Tong, H., Wang, H....glycosylase. Nature Communications, 15(1), 4897. https://doi.org/10.1038/s41467-024-49343-5 Tong, H., Wang...glycosylase. Nature Biotechnology, 41(8), 1080–1084. https://doi.org/10.1038/s41587-022-01595-6 Ye, L., Zhao...cells. Nature Biotechnology, 42(10), 1538–1547. https://doi.org/10.1038/s41587-023-02050-w Yi, Z., Zhang...glycosylase. Nature Communications, 15(1), 6397. https://doi.org/10.1038/s41467-024-50012-w Additional ...

  12. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...Blot for Anti-V5 [IPI-SV5-Pk1]. Addgene Report, https://doi.org/10.57733/addgene.l0ulu4. Hanke, T., Szawlowski...tag-linked antigen. J Gen Virol., 73 (Pt 3), 653–660. https://doi.org/10.1099/0022-1317-73-3-653. PMID 1372038... isolates. J Gen Virol., 68 (Pt 11), 2769–2780. https://doi.org/10.1099/0022-1317-68-11-2769. PMID 2445904...of protein degraders. Nat Commun., 15(1), 8885. https://doi.org/10.1038/s41467-024-52871-9. PMID 39406745...Caenorhabditis elegans. Genetics, 228(2), iyae126. https://doi.org/10.1093/genetics/iyae126.   Gene disruption...Mycobacterium abscessus. J Bacteriol., 206(3), e0033523. https://doi.org/10.1128/jb.00335-23.  Bioluminescent...Biological Systems. ACS Sensors, 9(7), 3489–3495. https://doi.org/10.1021/acssensors.3c02664. ...

  13. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...heterogeneous samples. bioRxiv 2024.01.21.576499; doi: https://doi.org/10.1101/2024.01.21.576499.    LEA proteins...plunge freezing. bioRxiv 2024.02.06.579238. doi: https://doi.org/10.1101/2024.02.06.579238.    Engineered...Biotechnology, 10.1038/s41587-023-02078-y. doi: https://doi.org/10.1038/s41587-023-02078-y.  Anzalone,...editing. Nature Biotechnology, 40(5), 731–740. doi: https://doi.org/10.1038/s41587-021-01133-w Pandey, S.,... Engineering, 10.1038/s41551-024-01227-1. doi:  https://doi.org/10.1038/s41551-024-01227-1   CHARM: A...epigenetic editor. Science, 384(6703), ado7082. doi: https://doi.org/10.1126/science.ado7082.  Nuñez, J. K....epigenome editing. Cell, 184(9), 2503–2519.e17. doi: https://doi.org/10.1016/j.cell.2021.03.025.    Recombinant...

  14. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...Chemistry, 15(7), 7. https://doi.org/10.1038/s41557-023-01214-0. Preprint on bioRxiv: https://doi.org/10.1101...organizer development. PLoS Genetics, 19(5), e1010765. https://doi.org/10.1371/journal.pgen.1010765.  Berlew,...Toolbox. ACS Synthetic Biology, 11(1), 515–521. https://doi.org/10.1021/acssynbio.1c00604.  Mahlandt, ...endothelial barrier strength. eLife, 12, RP84364. https://doi.org/10.7554/eLife.84364.  Meiring, J. C. M...disassembly. Current Biology, 32(21), 4660-4674.e6. https://doi.org/10.1016/j.cub.2022.09.010.  Ridley, A..... Current Opinion in Cell Biology, 36, 103–112. https://doi.org/10.1016/j.ceb.2015.08.005.  Tei, R., Bagde...

  15. Deep Dive: qPCR

    Type
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    .... Volume 14. 2017, Pages 19-28, ISSN 2214-7535, https://doi.org/10.1016/j.bdq.2017.11.001.Bustin SA; Benes...Volume 27, Issue 7, 1 April 2011, Pages 1019–1020, https://doi.org/10.1093/bioinformatics/btr065Freeman WM... Reprogramming Process. Sci Rep 8, 8716 (2018). https://doi.org/10.1038/s41598-018-26707-8Suzuki T; Higgins...publication quality data. Sci Rep 7, 2409 (2017). https://doi.org/10.1038/s41598-017-02217-xTaylor, SC; ...37, Issue 7, 2019, Pages 761-774, SSN 0167-7799, https://doi.org/10.1016/j.tibtech.2018.12.002.Teo IA; ... 28, Issue 19, 1 October 2000, Pages 3752–3761, https://doi.org/10.1093/nar/28.19.3752Wong M; Medrano ...mRNA quantitation. Biotechniques, 1, 30 (2018). https://doi.org/10.2144/05391RV01 Yuan JS; Reed A; Chen...

  16. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ... 8 and 9 in the Mouse Brain. PLoS ONE 8:e76310. https://doi.org/10.1371/journal.pone.0076310 Burger C,...Central Nervous System. Molecular Therapy 10:302–317. https://doi.org/10.1016/j.ymthe.2004.05.024 Dudek AM, ... Entry Independent of AAV Receptor. J Virol 92. https://doi.org/10.1128/jvi.02213-17 Fischer KB, Collins...vectors. Proc Natl Acad Sci USA 116:27001–27010. https://doi.org/10.1073/pnas.1915974116 Guerin K, Rego...Viral DNA Extracts. Human Gene Therapy 31:664–678. https://doi.org/10.1089/hum.2019.277 Lavin TK, Jin L, ...Virus Concentrations. Front Synaptic Neurosci 12. https://doi.org/10.3389/fnsyn.2020.00006 O’Carroll SJ,...Relevance to Human Gene Therapy. Front Mol Neurosci 13. https://doi.org/10.3389/fnmol.2020.618020 Additional resources...

  17. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...gene therapy.” Journal of cellular physiology.  https://doi.org/10.1002/jcp.30064 2. Barman et al. 2020...with CRISPR-Cas9 technology.” Current genetics. https://doi.org/10.1007/s00294-019-01040-3 3. Khatibi ... Review.” Current pharmaceutical biotechnology. https://doi.org/10.2174/1389201021666200621161610 4. Uddin...and Implications for the Future.” Front. Oncol. https://doi.org/10.3389/fonc.2020.01387 5. Rohn et al.... Journal of Alzheimer's disease & Parkinsonism. https://doi.org/10.4172/2161-0460.1000439 6. Wu et al....Therapy in Human Genetic Diseases.” Theranostics. https://doi.org/10.7150/thno.43360 7. Yuxuan Wu et al....

  18. CRISPR 101: Non-Homologous End Joining

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    ...Its Mutagenic Potential. PLoS Genet 5:e1000683 . https://doi.org/10.1371/journal.pgen.1000683 Bétermier...Inherently Error-Prone Process? PLoS Genet 10:e1004086 . https://doi.org/10.1371/journal.pgen.1004086 Ghezraoui...Nonhomologous End-Joining. Molecular Cell 55:829–842 . https://doi.org/10.1016/j.molcel.2014.08.002 Ran FA, Hsu...Genome Editing Specificity. Cell 154:1380–1389 . https://doi.org/10.1016/j.cell.2013.08.021 Waters CA, ...during nonhomologous end joining. Nat Commun 5: . https://doi.org/10.1038/ncomms5286 Yan M-Y, Li S-S, Ding...Editing in Mycobacterium tuberculosis. mBio 11: . https://doi.org/10.1128/mbio.02364-19 More CRISPR Blog...

  19. Antibodies 101: Conjugation

    Type
    Blog Post
    ... guide to cytometry. Trends Immunol 33:323–332. https://doi.org/10.1016/j.it.2012.02.010 Dennler P, Fischer...Populations to Defined Reagents. Antibodies 4:197–224. https://doi.org/10.3390/antib4030197  Hegazy M, Cohen-...Tissues in Situ. Curr Protoc Cell Biol 89:e115. https://doi.org/10.1002/cpcb.115  Mueller BM, WrasidloO... to Monoclonal Antibodies. Hybridoma 7:453–456. https://doi.org/10.1089/hyb.1988.7.453  Niemeyer CM, Adler...quantitative immuno-PCR. Nat Protoc 2:1918–1930. https://doi.org/10.1038/nprot.2007.267 Stoeckius M, Hafemeister...measurement in single cells. Nat Methods 14:865–868. https://doi.org/10.1038/nmeth.4380  Additional resources...

  20. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ... 1502–1513. doi: https://doi.org/10.1038/s41477-024-01786-w. (bioRxiv preprint: https://doi.org/10.1101...absence of cargo. bioRxiv 2024.12.20.629623; doi: https://doi.org/10.1101/2024.12.20.629623.  A fast and...synaptic events. Neuron, 112(22), 3680–3696.e8. doi: https://doi.org/10.1016/j.neuron.2024.08.019.  ONE-GO...biosensor platform. Cell, 187(6), 1527–1546.e25. doi: https://doi.org/10.1016/j.cell.2024.01.028. New recombinant...Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt.  ...
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