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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 187 results for: hwa

Showing: 61 - 80 of 187 results

  1. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ... in organoid cultures are stable,” said Gerald Schwank, first author of the cystic fibrosis report. “This...Cell. 13, 659-662 (5 December 2013). 8. Gerald Schwank et al. "Functional Repair of CFTR by CRISPR/Cas9...

  4. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...be applied to an important neuronal signalling pathway - if you’ve got a useful tool, there’s likely someway...networks, and selectively activating signaling pathways. Many scientists came to the Addgene booth to ...

  5. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...To control for protein degradation due to other pathways, it is standard practice to compare degradation...ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active form of...

  6. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...earning their PhDs (3), showing that the ‘standard’ pathway into academia may in fact be just another ‘alternative...recently launched a survey “Identifying Career Pathways for PhDs in Science”, through which she collects...

  7. Bacterial Expression Systems

    Type
    Collection
    ...ratio Fluorescence (mCherry) Mycobacterium sp. Ashwani Kumar 134405 pBbAW4k-loxP-TT-loxP-mRFP1 Cre recombinase...bacterial gene biology or engineering metabolic pathways to synthesize a target metabolite, genetic tools...

  9. CRISPR in the Clinic

    Type
    Blog Post
    ...isolated from two patients with cystic fibrosis (Schwank et al). They were able to demonstrate functional...Cas9." Cell Stem Cell. 13, 659-662 (2013). Gerald Schwank et al. "Functional Repair of CFTR by CRISPR/Cas9...

  10. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...further, his lab is now studying how Argonaute pathways may be involved in preventing silencing of self-genes...self-genes. Dr. Mello presented a hypothesis that these pathways are involved in RNA based surveillance of repetitive...

  11. Plasmids for Stem Cell Research

    Type
    Collection
    ...fibroblasts via microRNA-dependent and -independent pathways. EMBO Mol Med. 2017 Aug;9(8):1117-1131. Parmar...modulation of transcriptionally enriched signaling pathways to optimize the conversion of fibroblasts into...

  12. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ... using the endogenous homology directed repair pathway, a new user-defined DNA sequence (like GFP) can...previously unknown protein functions. References Schwartz, Matthew L. and Eric M. Jorgensen. “SapTrap, a...

  13. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...associated with rote memorization of metabolic pathways (precisely the type of exercise Einstein might...undergraduate research experiences (CUREs) as a pathway to diversify science. Evolution and Development...

  15. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ... exploiting the homology-directed repair (HDR) pathway which occurs in cells at low frequencies and relies...A, A>G, and T>C) installation by base editors. Pathways that compete with the desired outcome are not ...

  17. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...then cleaved by RISC and degraded. In the miRNA pathway, RISC doesn’t always cleave the mRNA — the bound...Comparison of shRNA and miRNA processing and silencing pathways. Created on BioRender.com.   Expressing shRNAs...

  18. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...and more, often driven by Rho family signaling pathways. Rho guanine-nucleotide exchange factors (GEFs...phosphatidic acid signaling (such as Hippo, AMPK and mTOR pathways) and to introduce unnatural head groups for applications...

  19. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...genome editing. The homology-directed repair (HDR) pathway is downregulated in mature tissues - no problem... through the non-homologous end joining (NHEJ) pathway. It’s estimated that very little dystrophin correction...
Showing: 61 - 80 of 187 results