We narrowed to 416 results for: iCre

...long dsRNA, typically hairpin RNA, or artificial microRNA). Using a DNA template for the RNAi trigger is... hpRNAs through Golden Gate cloning. Artificial micro RNA and short hairpin RNA The greatest limitations...shortcomings, both short hpRNAs (shRNAs) and artificial micro RNAs (amiRNAs) are frequently used. Although similar... are engineered from endogenous precursors of microRNAs found in the genome, whereas shRNAs are exogenously...hairpin RNA (shRNA) is conceptually similar to the microRNA precursor mentioned above and is a popular RNAi...limited, single cell protoplast analysis. Instead, microscopic plants must be visually identified as undergoing...the entire culture can be harvested instead of microscopic isolation. The plasmids required to conduct an...

... your plans. This situation may arise for a microbiologist, where cloning two plasmids into one bacterium...deal with the more sinister plasmids of the microbiological world. (Want to know more about these plasmid...beginning to use plasmid incompatibility to combat antimicrobial resistance. Using novel plasmids based partly... and Control of Circular Bacterial Plasmids. Microbiology and Molecular Biology Reviews 62(2): 434–464...assortment of pure plasmid clusters. Molecular Microbiology 56:1430–1440 . https://doi.org/10.1111/j.1365... plasmid incompatibility. Letters in Applied Microbiology 47:235–240 . https://doi.org/10.1111/j.1472-...19241516 Novick RP (1987) Plasmid incompatibility. Microbiology Reviews. 1987;51(4):381–395. Pubmed PMID: 3325793...

...- a chemically stable tool for microscopy by: Susanna Stroik Microscopy techniques have been moving at...advanced microscopy applications such as super resolution, electron, and expansion microscopy. hfYFP is...hfYFP in expansion microscopy as well as correlative light and electron microscopy and observed high fluorescence...re looking for a super stable FP for your next microscopy experiment, consider hfYFP and LSSmGFP.   ...Chemically stable fluorescent proteins for advanced microscopy. Nat Methods 19, 1612–1621 (2022). https://doi.org...

...contributed by guest blogger, Jessica Sacher, a microbiology PhD student at the University of Alberta studying..., see Simpson et al., 2015). Visit Addgene's MIcrobiology Pages The problem: Host binding proteins are... blogger Jessica Sacher! Jessica Sacher is a microbiology PhD student at the University of Alberta studying...new applications for old resources." Trends in microbiology 23.4 (2015): 185-191. PubMed PMID: 25708933....bacteriophage-based therapeutics."Frontiers in Microbiology 7 (2016). PubMed PMID: 27536293. PubMed Central...metagenomic screening." Applied and environmental microbiology 74.5 (2008): 1649-1652. PubMed PMID: 18083885...Learn How CRISPR Can Be Combined with Phage for Antimicrobial Purposes Use Yeast Two Hybrid Systems to Probe...

...using i-GONAD (15%) vs. microinjection delivery (25-67%). However, microinjection does require 2.5x more...for clones with the recombined product, you’d microinject into blastocysts to be implanted in pseudopregnant...efficiency. Easi-CRISPR is compatible with standard microinjection and electroporation methods using in mouse ...and delivery methods Using RNP delivery and microinjection, the efficiency of Easi-CRISPR for simple knock-ins...anatomy with the equipment used for i-GONAD. A micropipette is used to inject solution into the oviduct ...components to the germline. In contrast to standard microinjection, i-GONAD is performed directly on pregnant ...electroporation. Ohtsuka et al. compared traditional microinjection and i-GONAD knock-in efficiency using a small...

..., check out the OpenWorm website. microPublication microPublication is a unique publication for the worm...otherwise be lost in lab notebooks forever. microPublications are peer-reviewed and assigned a DOI that ...referenced. In addition the data published in microPublications is curated and added to other databases including...including wormbase and flybase. microPublication is run and maintained by members of the worm community... four-dimensional contrast interference (DIC) microscopy and computer imaging processing. The combination...introducing point mutations that was published in a microPublication (Schwartz and Jorgensen, 2018).  These are...point mutations with unc- 119 + selection." MicroPublication 1 (2018): 2–3. Wang, Han, et al. "cGAL, a ...

...and electron microscopy (CLEM) is an imaging technique that combines electron microscopy (EM) with fluorescence...the second, and vice versa. Finally, as every microscope will have it’s own quirks (level of zoom, intensity...to revolutionize the field of super resolution microscopy. PA-FPs, especially the monomeric, reversible... Indeed, within the world of super resolution microscopy, PA-FPs have helped create several new imaging...methods, including photoactivation-localization microscopy (PALM, also FPALM) (11). For PALM, a small area...engineered. By combining PA-FPS with superresolution microscopy, scientists now have at their fingertips the ...mCherry for high-resolution two-color fluorescence microscopy." Nature methods 6.2 (2009): 153-159. PubMed ...

...Strategic immune evasion mechanisms - The tumor microenvironment Through interactions with the immune system...and thrive within what is known as the tumor microenvironment - a vital atmosphere created by the cancer...to mature into effector cells in the tumor microenvironment. Tumor cells in this environment produce growth...recruit regulatory T cells (T-regs) into their microenvironment by producing C-C motif chemokine 22 (CCL22...function. Creating Tolerance T-regs in the tumor microenvironment produce CTL associated protein 4 (CTLA-4),... above. Finally, signals released from the microenvironment help create tolerance beyond direct proximity...growth and subjecting patients to the risk of microbial infection. Chronic inflammation from infection...

...whistles’ – including microscale light emitting diodes, thermal sensors, and microfluidic channels. These features...neural fibers developed by the Anikeeva lab are microelectronic fibers made from thermoplastic polymers that...desired thickness. The fibers are drawn with microelectronics included in them for downstream control – ...(Orguc et al., 2020). Using a programmable microcontroller, NeuroStack coordinates optical stimulation...A., … Anikeeva, P. (2023). Multifunctional microelectronic fibers enable wireless modulation of gut and...resources on the Addgene blog Plasmid tools for microbiome studies Channel rhodopsins for optogenetics Viral...

...sterilization as mycoplasma can even escape 0.2-micron filters. Figure 1: Contamination! Tissue culture...media that turns yellow from lowered pH due to microbial contamination. Photo by Kaustubh Kishor Jadhav...Detection of mycoplasma by naked eyes or optical microscope can be very difficult, so how do you know it’...mycoplasma. When observed under a fluorescent microscope, the mycoplasma DNA appears in the form of small...lines for assay or analytical purposes. Use 0.1-micron filters as are they are a better way of avoiding...avoiding mycoplasma, rather than using 0.2-micron filters, for media filtration. There is no 100% assurance...the Detection of Mycoplasmas Using Fluorescence Microscopy. Cells 8:1510 . https://doi.org/10.3390/cells8121510...

...Fluorescence Microscopy. iBiology. https://www.ibiology.org/talks/fluorescence-microscopy/   Uhlen M, ...Learn more about fluorescence microscopy in Which Fluorescence Microscopy Techniques is Best for Me? Read...commonly proteins. You can then use fluorescent microscopy to look for those fluorescent molecules, knowing...fluorescence, try the Introduction to Fluorescence Microscopy video from iBiology). IHC and ICC are essentially.... The methods used depend on the sample type, microscopy tools available, and your experimental goals....

...Plasmids and Chromosomes. Microbiol Spectr. 2014 Oct;2(5). doi: 10.1128/microbiolspec.PLAS-0025-2014. PMID: 26104344...Behavior of Plasmid DNA. Microbiol Spectr. 2015 Apr;3(2):10.1128/microbiolspec.PLAS-0036-2014. doi: 10.1128...10.1128/microbiolspec.PLAS-0036-2014. PMID: 26104708; PMCID: PMC4480603. Holovics HJ, He Y, Lacher NA, Ruesch...the secrets of multicopy plasmid stability. Mol Microbiol. 1998 Sep;29(5):1137-45. doi: 10.1046/j.1365-2958.1998.01012...instability: the dimer catastrophe hypothesis. Mol Microbiol. 1993 Jun;8(6):1031-8. doi: 10.1111/j.1365-2958.1993...

...begins almost 50 years ago with the discovery of microbial rhodopsins, light-sensitive proteins that absorb...intercellular signaling pathways (Ernst et al., 2014). Microbial rhodopsins are encoded by opsin genes, and include...to visible light (Zhang et al., 2011). What do microbial rhodopsins and neurons have in common? Neuronal... light: when hit with the correct wavelength, microbial rhodopsins enable ions to flow across the axon... inject genetically modified viruses encoding microbial rhodopsins into specific cerebral regions. The...PubMed PMID: 31082625. Ernst, Oliver P., et al. "Microbial and animal rhodopsins: structures, functions, ...Central PMCID: PMC5830081. Zhang, Feng, et al. "The microbial opsin family of optogenetic tools." Cell 147.7...

...visible under both phase contrast microscopy and electron microscopy (Fig. 1b). Gas vesicle expression...inclusions in cells with GVs via phase contrast microscopy, GVs visible in cells via TEM, and purified GVs...detectable under ultrasound and phase contrast microscopy just one day after induction of expression… but...positivity, which can be observed under phase contrast microscopy and TEM (Fig. 2c), and ultrasound imaging. If...Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts. Nature 553, 86–90 (2018...

...molecule interactions in living cells using a microscope. Historically, molecular interactions were detected...the acceptor fluorophore can be measured using microscopy techniques. FRET measurement sensitivity makes... method and the fluorescence lifetime imaging microscopy (FLIM) method. Acceptor photobleaching method...donor molecule. Fluorescence lifetime imaging microscopy method FLIM has been developed more recently ...RN, Davidson MW. Fluorescent proteins for FRET microscopy: monitoring protein interactions in living cells...Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations. J...

... funding. Using current resources to develop microbiology and molecular biology research programs. 30:... public about dietary health. 40:17 - 44:14 : Microbiome research - working with bacterial communities...- end : Kwabena's time in Boston - Developing microbiome expertise. Many thanks to Kwabena Duedu for ...Kaba Resources on Addgene.org Check out our Microbiology Pages Find Synthetc Biology Plasmids Find Molecular... microbiology...

...appreciated on an aesthetic level as well. Beautiful microscope images which traditionally languished in the ... can be found on his website http://www.microworldsphotography.com/. Another artist who spent his life...you’re a scientist who spends many hours at a microscope, you too can become an artist. Scientists moonlighting...artists can compete in the Nikon Small World Photomicrography Competition - a 40 year old international ...and science? Do you look for the beauty in the microscopic organisms on your lab bench? Let us know in the...

... Plasmids! Light controls the activity of a microtubule-associated protein to regulate cell growth dynamics...light by modifying EB1. EB1 normally sits on microtubules to attract other proteins involved in their ...into two halves (Fig. 1): one containing the microtubule binding domain and the second containing the ...modified EB1 protein are overexpressed in cells, microtubule growth can be switched off with blue light which...mCherry-positive to eGFP positive cells through fluorescence microscopy or flow cytometry. Using the ACE system, the ...

... biological dark matter – large tracts of the microbial tree of life that remained unexplored. Using new...axenic (pure) laboratory cultures to study these microbes. From 16S rRNA sequencing, hot springs were known...YNP). Extremophiles - phylogenetically diverse microbes living in extreme physical and chemical conditions...tiring. It feels a bit strange to be using a micropipette and gloves at a campsite. And it was exciting.... They used a mini-metagenomics method using microfluidic parallelization to sequence this highly complex...

...expert in microbial ecology but she became a member at BosLab because she wants to engineer microbes. We’ve...Laurenceau, and Tami Pudina have initiated a soil microbiome project. This project was inspired by previous...it, and analyze the data to characterize the microbiomes of the different samples. The goal is to not ...not only learn something about the microbial diversity in different soil conditions/locations but to engage...