Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 69 results for: pcas9

Showing: 61 - 69 of 69 results

  1. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...purposes. NOTE: Here we discuss the usage of the pSpCas9(BB) plasmid (pX330) (Addgene plasmid ID 42230)....allows for the simultaneous expression of sgRNA and SpCas9, but does not contain markers for selection 1 ....

  2. CRISPR Guide

    Type
    Guide
    ...pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant 3' NGG (reduced NAG binding) SpCas9 VRER variant 3'...NGCG SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3...engineered Cas9s can enhance CRISPR specificity. SpCas9 (from Streptococcus pyogenes ) is the most popular...no cuts. Cas9 nickase (Cas9n), a D10A mutant of SpCas9, has one active nuclease domain and one inactivated...fidelity enzymes, and their mechanisms, include: eSpCas9(1.1) - weaken interactions between the HNH/RuvC...RuvC groove and the non-target DNA strand SpCas9-HF1 - disrupt Cas9’s interactions with DNA phosphate backbone...your desired genes for modification. Engineered SpCas9 enzymes with altered PAM specificities are available...

  5. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...binding variant of Cas9. For instance, wild-type SpCas9 must be used with targets that are upstream of ...

  6. Plant Plasmids and Resources

    Type
    Collection
    ...Kit includes a high efficiency intron-optimized SpCas9-coding gene, zCas9i, and an intronized Cas9 series...

  7. Validated gRNA Sequences

    Type
    Collection
    ... or PAM binding variant of Cas9 (e.g. Wild-type SpCas9 must be used with targets that are upstream of ...
Showing: 61 - 69 of 69 results