We narrowed to 67 results for: pcas9

1. CRISPR Guide

   Type

   Guide

   ...pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant 3' NGG (reduced NAG binding) SpCas9 VRER variant 3'...NGCG SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3...engineered Cas9s can enhance CRISPR specificity. SpCas9 (from Streptococcus pyogenes ) is the most popular...no cuts. Cas9 nickase (Cas9n), a D10A mutant of SpCas9, has one active nuclease domain and one inactivated...fidelity enzymes, and their mechanisms, include: eSpCas9(1.1) - weaken interactions between the HNH/RuvC...RuvC groove and the non-target DNA strand SpCas9-HF1 - disrupt Cas9’s interactions with DNA phosphate backbone...your desired genes for modification. Engineered SpCas9 enzymes with altered PAM specificities are available...

2. 22 Hot Plasmid Technologies from 2014

   Type

   Blog Post

   ... From the lab of Yonglong Chen, pCS2-3xFLAG-NLS-SpCas9-NLS is a Cas9 expression plasmid that was used ...

3. CRISPR Plasmids - gRNAs

   Type

   Collection

   ...binding variant of Cas9. For instance, wild-type SpCas9 must be used with targets that are upstream of ...

4. Plant Plasmids and Resources

   Type

   Collection

   ...Kit includes a high efficiency intron-optimized SpCas9-coding gene, zCas9i, and an intronized Cas9 series...

5. Validated gRNA Sequences

   Type

   Collection

   ... or PAM binding variant of Cas9 (e.g. Wild-type SpCas9 must be used with targets that are upstream of ...

6. Neurodegeneration Plasmid Collection

   Type

   Collection

   ...-ko-sgRNA1-SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 TARDBP U6 ALS...

7. Adeno-associated virus (AAV) Guide

   Type

   Guide

   ...Packaging the Cas9 from Streptococcus pyogenes (SpCas9) and a gRNA together into an rAAV capsid is challenging...