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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 289 results for: poly

Showing: 61 - 80 of 289 results

  1. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ... sample and subject to isothermal recombinase polymerase amplification (RPA) using a commercially available...reaction also contains SSB, strand displacement DNA polymerase, recombinase, and the ssDNA-FQ reporters that...transcription. This is then amplified by PCR using Taq Polymerase and then transcribed to provide RNA substrates...instead of RPA. Unlike RPA, this technique uses Taq polymerase which is widely available and less expensive....

  2. Plasmids 101: Biotinylation

    Type
    Blog Post
    ... biology and biotechnology purposes.  Once a polypeptide is biotinylated (more on this below) it can be...Northern blotting Cell surface labelling Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated...to either terminus of a DNA sequence during a Polymerase Chain Reaction (PCR) by using a biotinylated ...commonly used this method to attach proteins or polypeptides to a solid substrate (Holden and Cremer, 2003...

  3. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...single stranded regions with homology to anneal a polymerase, to fill in the gaps a DNA ligase, to seal the...than 200 nucleotides before the annealing and polymerization steps could occur. Secondly, it does not work...instead of using the exonuclease feature of T4 DNA polymerase, which must be controlled by the presence or ...methods that have been described--CPEC (circular polymerase extension cloning) and SLiCE (Seamless Ligation...

  4. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...join any fragments end-to-end without the exo/polymerase generation of annealed complementary regions....and even include profiling single nucleotide polymorphisms (SNPs) (5,12,13). For example, in the Ligase...Shuman, Stewart, and Christopher D. Lima. "The polynucleotide ligase and RNA capping enzyme superfamily of...for characterization of resistance-mediating polymorphisms in African samples of Plasmodium falciparum....

  6. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ... uses do you foresee for this tool? Howarth: Polyproteins are useful for mechanical study of proteins ...proteins by atomic force microscopy (AFM). Maybe polyantibodies could help in the capture of other rare cell ...References: SpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture...

  7. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... a duplicated SV40 polyadenylation region instead of the synthetic polyadenylation signal present in pGL3...recruits up to 24 copies of a protein to a target polypeptide. Multimerization in the SunTag system occurs ... simplifies the process of creating complex polycistronic lentiviruses. The MuLE toolbox contains a series...constitutive promoters, 2 includible promoters, 3 polyA terminators and various pieces for inducible expression...UAAs via a re-assigned stop codon (TAG). This polyspecificity enables the site-specific introduction of many...

  8. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...microelectronic fibers made from thermoplastic polymers that can work with all the bells and whistles....called thermal drawing, essentially where blocks of polymers are heated up and pulled to a desired thickness... small molecules.   Fun fact: A 1 cm block of polymer can generate many meters of fibers!     ...

  9. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ... bodies are a polymer of many copies of monomeric subunits. Unlike cytoskeletal polymers, however, R bodies...force by dynamically rearranging into different polymerization states. Instead, R body monomers respond to...

  10. Microbiology Resources

    Type
    Collection
    ...vaccinia virus - Bennink and Yewdell Labs Yarrowia lipolytica Golden Gate tool kit : Integrative vector assembly...transcription units in the oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors...

  12. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Adeno X shuttle vector with EF1 promoter and SV40 polyA pICPIS-CB 73356 AdenoX shuttle vector with chicken-beta...chicken-beta actin CMV enhancer promoter SV40 polyA...

  13. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ...fragments are then combined with an exonuclease, a polymerase, and a DNA ligase to carry out a pooled set of...topoisomerase I cut site, toposisomerase I, and a taq polymerase-generated insert. When mixed, topoisomerase I... could also be a great choice, because the T4 polymerase needed for this method is relatively inexpensive...

  14. Plasmids 101: Protein Expression

    Type
    Blog Post
    ... can then be translated at the ribosome into polypeptide chains that make up the primary structure of ...purified for use in molecular biology research (eg. polymerases and other enzymes might be purified and used ...translation machinery. These include ribosomes, RNA polymerase, tRNAs, ribonucleotides and amino acids. Cell-free...

  15. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...Addgene blog   Droplet Digital PCR for AAV QuantificationPolymerase Chain Reaction Overview and Applications...Applications Resources on Addgene.org Protocol: Polymerase Chain Reaction (PCR) Protocol: How to Design a primer...SYBR Green I and TaqMan quantitative real-time polymerase chain reaction methods for the determination ...

  16. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...set up a standard PCR reaction (primers, dNTPs, polymerase) using the supernatant of lysed bacteria as template...standard PCR reaction: combine template, primers, polymerase, and dNTPs and then incubate with a standard ...heating step of the PCR reaction. A standard Taq polymerase is sufficient. Controls: Controls can make ...

  17. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ... separate plasmid (envelope plasmid), and the polymerase (Pol) for replication and Gag structural protein... be disposed of following your lab’s biohazard policies. Administering Virus Many virus experiments involve... accident-prone researchers. Note that safety policies will vary slightly from organization to organization...with your specific organization/department/lab’s policies – basically, you’ll need to get trained in each...

  18. Viral Production at Addgene

    Type
    Blog Post
    ...Samples of viral preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver...centrifugation and then by filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then ...lentiviral preparation by precipitation in 10% polyethylene glycol (PEG) followed by centrifugation. Precipitated...

  19. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...with a “sloppy” version of PCR that results in a polymerase error rate of up to 2% per nucleotide position...modifications: 1) increased concentration of Taq polymerase, 2) increased PCR extension time, 3) increased...degenerate primers and the high-fidelity Phusion polymerase are used to introduce point mutations into the...

  20. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...experiments. shRNA plasmids typically use RNA polymerase III promoters, which transcribe small RNAs. The..., shRNA plasmids are being designed using RNA polymerase II promoters. The design of these plasmids is...(2004). MicroRNA genes are transcribed by RNA polymerase II. The EMBO Journal, 23(20), 4051–4060. https...
Showing: 61 - 80 of 289 results