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TypeProtocol...may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service Introduction This...
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Adeno-associated virus (AAV) Guide
TypeGuide...in rodents and non-human primates. Read our blog post on systemic capsids for more information. Browse...better understand brain structure. Read our blog post on AAVrg for more information. Self-complementary...more about these and other initiatives in our blog post on using AAV for neuronal tracing . Browse AAV vectors...optimized for their use in rAAV vectors . Read our blog post on overcoming AAV size limitation for CRISPR delivery...development of further AAV applications. Read our blog post on split AAVs for more information. Viral Vector...packaging. WPRE in cis Woodchuck hepatitis virus post‐transcriptional regulatory element; stimulates the... -
AAV Purification by Iodixanol Gradient Ultracentrifugation
TypeProtocol...may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Introduction This protocol can... -
Coomassie Purity Stain of Recombinant Antibodies
TypeProtocol...like... Antibody Plasmid Collection Antibody Blog Posts Antibody Guide Transfection for Recombinant Antibodies... -
Western Blot
TypeProtocol...like... Antibody Plasmid Collection Antibody Blog Posts Antibody Guide Immunocytochemistry Protocol Recombinant... -
Guide to Using Pooled Libraries
TypeGuide...the goal is to identify those cells that survive post-selection. The selective pressure must be strong...our Viral Vector resources . Check out our blog posts featuring some of our most popular libraries! Genome-wide... -
Pouring LB Agar Plates
TypeProtocol...Agar Plates You may also like... Plasmids 101 Blog Posts Mol Bio Protocols Cloning Protocols Introduction... -
AAV Titration by qPCR Using SYBR Green Technology
TypeProtocol...may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Introduction The AAV Titration... -
Isolating a Monoclonal Cell Population by Limiting Dilution
TypeProtocol...may also like... Viral Vector Guides Virus Blog Posts Addgene Protocols Viral Service Introduction This... -
Kit Free RNA Extraction
TypeProtocol...For more tips on working with RNA, read this blog post on RNA extraction without a kit . Last Update: April... -
Affinity Purification of Recombinant Antibodies with Protein A or Protein G
TypeProtocol...like... Antibody Plasmid Collection Antibody Blog Posts Antibody Guide Transfection for Recombinant Antibodies... -
Antibody Validation Using the Indirect ELISA Method
TypeProtocol...like... Antibody Plasmid Collection Antibody Blog Posts Antibodies 101: ELISA Antibodies 101: The Four ELISAs... -
Educational Resources
TypeGuide...Educational Resources From eBooks to videos and blog posts to protocols, our educational resources cover a ... -
Genetic Code Expansion
TypeCollection...to the GCE bulletin board (GCEbb) listserve and posting your questions there. This peer-to-peer GCE community... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR requires that you have to express both a ..._LacZ 74293 Mammalian S. pyogenes Neo, mCherry Postovit pdCas9-DNMT3A-PuroR_v2 74407 Mammalian U6 yes,... -
Validated gRNA Sequences
TypeCollection... CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The table below lists gRNA sequences that have been... -
CRISPR Library Amplification
TypeProtocol...mL SOC per electroporation readily available for post-electroporation recovery of cells. Ensure access... -
Lentivirus ddPCR Titration
TypeProtocol...Addgene Viral Service Lentivirus Guide Virus Blog Posts Introduction This protocol describes how to use ... -
A Conference By Postdocs For Postdocs: Future of Research
TypeBlog Post...This post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates...) Read more Science Career Development posts ... of Research conference is organized by postdocs for postdocs. I am delighted to see enterprising early...changes to the way we view the graduate school and postdoc training years. There is no doubt that there are...appeared in eLife: "A Fair Deal for PhD Students and Postdocs" by Dr. Henry Bourne, Professor Emeritus at UCSF...know of many schools that have completely student/postdoc-driven biotechnology clubs such as Penn State, ...the grants and programs they need such as at the Postdoc Career Development Initiative (PCDI) in the Netherlands... -
Cre-lox system
TypeCollection..., check out Addgene’s Plasmids 101: Cre-lox blog post. Based on these Cre-lox recombination principles...