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...very interesting post about how we develop our in house lab management software. The post is written in ... Read All Our Editor's Choice Blog Posts The Addgene Blog has had quite the up-tick in views over the... Dewari who wrote this month’s most popular blog post on early user experiences with CRISPR alternative...detailed results of his survey can be found in the full post but TLDR: many users have had difficulty with NgAgo...editor’s choice picks this month, I’m picking two posts that describe methods to make single base edits ...generate many mutated plasmid constructs. The second post was contributed by Addgene Scientist Mary Gearing...cells are all discussed further in this informative post. Interestingly, this technology may make some uses...

... sequence. WPRE in cis Woodchuck hepatitis virus post‐transcriptional regulatory element; stimulates the...

...contestants!           View this post on Instagram                     A post shared by Xi Chen (@seleena78...2022          View this post on Instagram                     A post shared by 草方 (@gracie_fang)...of.           View this post on Instagram                     A post shared by Xi Chen (@seleena78...seleena78)   Here’s how to enter: Post your picture or video for either contest on Twitter or Instagram by...the hashtag #DeckTheLab, and tag @Addgene in the post! In January, Addgenies will vote on the entries...

...writing the first post so I signed up to write a second. While working on this post, I was offered and...blog post outlines 10 steps to take when starting to use Twitter as a scientist. While this post is designed...shared in the post. And the blog writing tips are elements I try to include in each post I write for the...professional advantage. It wasn’t until I wrote a blog post about a research paper that explored how scientists...started with the Addgene blog. I wrote my first blog post out of boredom. I had just started working in a ...When I saw a request for an Addgene guest blogger post about colony PCR, I signed up immediately. I had...Twitter, I found this paper. I wrote an Addgene blog post about it and shared the paper with my lab since ...

...blog this year. Spoiler alert: many of the top 10 posts from this year were influenced by COVID-19 and our...new cloning grade DNA service. We've had 11 blog posts on COVID-19 featuring plasmid tools from depositors...further ado, here are the top ten! Top 10 blog posts from 2020 10. What's the Best Way to Elute and Store...about temperature? Find the details in this blog post. 9. Droplet Digital PCR for AAV Quantitation by...titers. Get the details on the methods in this blog post. 8. Chromoproteins: Colorful Proteins For Molecular... the year, Joanne Kamens followed up with a blog post with creative ways to continue career development...can't stably maintain them in the cell. This blog post features two mechanisms that cause plasmids to be...

...methods in this blog post. Hope you’ve enjoyed going through some of these top blog posts. We’d also like ...the most recent plasmid blog posts Find the most recent CRISPR blog posts Resources at Addgene Browse...page. So let’s get to it. Here’s the top 10 blog posts from the year. 10. Hot Plasmids - March 2019 - Anti-CRISPR...finding your clones in an experiment. Read this blog post for some positive selection (ex: antibiotic selection.... Learn more about the aptamer soup in this blog post. 5. Five Popular Model Organisms by Alyssa Cecchetelli...people are using for their research. In this blog post, we’ve covered five model organisms: mouse, fruit...

...Changing Labor Laws Bring Increased Postdoc Wages Our most popular blog post this month shares incredibly important... Read All of Our Editor's Choice Blog Posts Last month there was plenty of excitement at Addgene. Our... laws and their possible repercussions in Gary’s post. Grad School Advice Part 2: Building Community  ... School Advice Part 2: Building Community.” This post leads readers to our latest podcast all about graduate... your PhDs. Of course there were many fantastic posts in October. Click on the links below to catch up...use Reddit to discuss your science and more! All Posts from October 2016: Sequencing Options for CRISPR...for anyone considering a postdoc. In “Changing Labor Laws Bring Increased Postdoc Wages,” Future of Research...

...to you. Many job postings will result in hundreds of applicants the day they are posted.  If your resume... requested in the posting Do this online and/or via email exactly as the job posting instructs even if...most job seekers will and should apply for jobs posted online as one part of their job search. This is...to nail the job yourself.   See the links in the post for additioanal advice for the science career search...search.  You might also enjoy the other posts in the Addgene Blog Scientist Careers Series.    ...

... and are typically free of charge for authors to post. They can be assessed and commented on by readers...Early-career researchers especially enjoy the ability to post a preprint as evidence of their productivity that...modes of scientific communication. Researchers often post a preprint at the same time as, or before, submitting...present some unpublished results at a conference? Post your working manuscript as a preprint too and you...subscriptions to the specialized journals in your field? Post a preprint before you submit to a journal and share...The most often-cited reasons researchers might not post a preprint are reluctance to share their work too...of preprints. Some worry about faulty work being posted as preprints, assuming that peer review is the ...

...their job postings and salary expectations public, so start by looking at these university postings to give...This post was contributed by Theresa Liao of the University of British Columbia. When transitioning from...than 2 pages long. Write your resume for the job posting Don't think a resume is "one size fits all" like...your resume, and tailor the resume for the job posting. For example, if the job asks for experience in...analysis” (word by word) in your resume. If the job posting says “French-speaking is an asset,” mention “speaks...next step. Start thinking now. Start browsing job postings and look at the jobs you are interested in. Consider...Academic Job Search Handbook & Resumes  Read Our Blog Post on Submitting a Resume That Gets Seen Thank You...

...can’t do it all ourselves. Therefore, in this blog post we’re taking a moment to reach out to you, our readers...editors will work with you to help hone your blog post for our audience of biological scientists from a... way to get your name out there! Completed blog posts are also great to include on CVs and Resumes. An...interested in applying CRISPR for mouse genome editing. Post: Generating Mouse Models Using CRISPR/Cas9   Erik...Professor at Albert Einstein College of Medicine. Post: When is a Monomer not a Monomer? The Top Three ...

... GelRed Pre and Post (Post recommended) UV Light 0.1 GelGreen Pre and Post (Post recommended) UV or...and Post Visible Light 100 Methylene Blue Post Visible Light 100 Ethidium Bromide Pre and Post UV ...  Stain Pre or Post Run? Visualization Sensitivity (ng DNA) SYBR Safe Pre and Post Blue or UV Light...samples or add the stain as the gel is being made (post or pre run in the table above, respectively). Some... will allow you to read over the insert. See our post on how to verify your plasmid for more details. ...Golden Gate or SLIC Cloning Read Other Plasmids 101 Posts Resources on Addgene.org Find the Perfect Empty...

...could see the questions being posted as they were coming in (the AMA was posted at 8:00 am and Joanne didn...Reddit, but it wasn’t until one of our blog writers posted his article (Developing Lab Management Software...pretty strict rules about not being too sales-y in posts which falls in line with our general mission to ...evergreen! With all the great questions that were posted, Joanne’s AMA is now a great resource for any scientists...AMA in the future and link to it in future blog posts on these topics. Others can also continue the conversation... communicating face to face with the audience. I post on LinkedIn where profiles are not at all anonymous...other science career topics. However, many of the posts were about toxic advisors, fruitless job searches...

... I’ve compiled a list of the Top 10 Most Viewed Posts on our blog from the past year. A quick glance shows...technique has developed and improved. The CRISPR posts written by our guest bloggers, the experts in the...know what topics you want to learn more about. Our post on “Making Your Own Competent Cells” has been extremely.... If you haven’t yet read the following Top 10 posts of 2014, take some time and see what you’re missing...addgene.org if you are interested. Addgene's Most Popular Posts of 2014 How to Design Your gRNA for CRISPR Genome...

...the list is growing. Some post positions as they come up, whereas others post their positions all at once...Updated Apr 6, 2021. This post was contributed by Laura Desrochers, a postdoctoral scientist at AstraZeneca..., have specific web sites for postdoc listings, so the method of posting varies between companies. Like...all careers related blog posts here Find 9 tips for a successful postdoctoral experience Read Joanne Kamen's...seen listed as a requirement on some industry job postings. Also, if you work in industry for any length ...like to work on later. For instance, AstraZeneca posts most of its positions all at once at the end of ...AstraZeneca. Why I chose an industry postdoc I’m currently doing an industry postdoc in neuroscience at AstraZeneca...

...out time during my day to write a blog post, edit other blog posts, and contact PIs for an upcoming visit... draft of post Reach out to Beth to see how the interviews are going Read over Leila’s post Read over ...over Susanna’s post Upload Eric’s post and give it a final read Browse list of previous depositors from Boston... have draft blog post ready for publication for tomorrow, I’d better upload that post from Eric before... in need of a few ideas, I hope you’ll find this post useful. After a bit of research and thinking about... need to do by 6 pm. Hopefully you’ll find this post useful for making your days, experiments, and projects...

...Addgene blog? Or why we make certain videos? In this post, we invite you behind the scenes in our content ...uncommon to see requests for fact checks on a blog post or Tweet, followed by very detailed answers from...our incredible scientists. Expert reviews on blog posts are done on a case-by-case basis, as many of our...research and previously written Antibody 101 blog posts were used, as well as consults with microscopy and...designer not shown.   However, for our blog posts, we usually make figures in BioRender or use appropriately...gaps and add to them as techniques evolve. Blog posts get out of date as techniques and knowledge changes...blog topics or even reach out about writing a guest post (email [email protected].) We are here to support...

... Most proteins are then modified via an array of post-translational modifications including protein folding...synthesis of proteins from mRNA and the addition of post-translational modifications Learn more molecular... plans for downstream applications In this blog post we will summarize some of the more common expression...expression of mammalian proteins that require multiple post-translation modifications for proper protein function...promoters (SV40, CMV, and RSV) for robust expression post-transfection. Mammalian systems can express proteins...produce complex eukaryotic proteins with the correct post-translational modifications. There are two types...bacterial cells are not equipped to add appropriate post-translational modifications. In addition many proteins...

...who shared his flow cytometry expertise in three posts. An Introduction to Gating in Flow Cytometry, Designing... who shared her flow cytometry expertise in two posts. Flow Compensation and Flow Cytometry Controls ...critical to the development and writing of two blog posts. Plasmids 101: Modular Cloning and Plasmids 101...More resources on the Addgene blog 10 Great Guest Posts We're Thankful For Lucky Thirteen: Guest Contributors...

...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at theJanelia... example). This blog post is about how to get the most out of your postdoctoral training experience. ... successful postdoc fellowship. 1. Reverse engineer success On Day 1 of your postdoctoral experience,...your postdoc At the end of grad school, you searched for and eventually identified a postdoctoral mentor...the Howard Hughes Medical Institute. Today's postdoctoral fellows (PFs) face a number of challenges ranging...academia - the main reason most people enter postdoctoral training. Similarly, there are several factors...factors to consider when selecting a postdoc mentor and lab. These topics have been addressed in numerous...