Addgene

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Tips for Titering Your Lentiviral Preps

Type

Blog Post

...long term use, you may want to consider using an aliquot that has been subjected to a freeze/thaw cycle ...
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

Type

Blog Post

...delivery may also be achieved via injection into the liquor of the cerebral ventricles (ICV, intracerebroventricular...
Hot Plasmids and Viral Preps - March 2021

Type

Blog Post

...service By Jason Nasse We regularly add new viral aliquots from our plasmid collection to provide ready-to-use...
Hot Plasmids and Viral Preps - January 2021

Type

Blog Post

...service By Jason Nasse We regularly add new viral aliquots from our plasmid collection to provide ready-to-use...
Hot Plasmids - October 2020

Type

Blog Post

...service By Jason Nasse We regularly add new viral aliquots from our plasmid collection to provide ready-to-use...
Pooled Library Amplifications

Type

Blog Post

...plasmid diversity, so Addgene strives to distribute aliquots of the original library whenever possible. In ...
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

Type

Blog Post

...superfolder, Emerald, CFP, YFP, BFP, Cerulean, Turquoise, Venus, and Citrine) can be monomerized with an...
10 Basic tips for mammalian cell culture

Type

Blog Post

...starting off with a new cell line, grow the cells, aliquot them into a number of vials, freeze them, and store...
Hot Plasmids and Viral Preps - May 2021

Type

Blog Post

...service By Jason Nasse We regularly add new viral aliquots from our plasmid collection to provide ready-to-use...
Which Fluorescent Protein Should I Use?

Type

Blog Post

...Fluorescent Protein (GFP), a protein from the jellyfish Aequorea Victoria. Since that discovery, the family of ...
Droplet Digital PCR for AAV Quantitation

Type

Blog Post

... from where you prepare your sample dilutions. Aliquot all of your reagents into single-use tubes and ...
Simplify Cloning with in vivo Assembly

Type

Blog Post

...than making up your PCR mix every time, pre-made aliquots of PCR master-mix, containing buffer, polymerase...
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

Type

Blog Post

...amino acids derived from the crystal jellyfish Aequorea victoria. It has a fluorescent emission wavelength...
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

Type

Blog Post

... all the bins together at a mixer. We call this Quorum Sensing, after the phenomenon in bacteria that ...
An Inside Look into Addgene's Dev Team

Type

Blog Post

...and in other places, we might be calling it an aliquot. This can lead to confusion as to what the code...
Getting the Most from Your Lentiviral Transduction

Type

Blog Post

... viral preps should be divided into single-use aliquots, and stored at -80°C. Some reports suggest rapidly...
How to Write a Scientific Review Article

Type

Blog Post

...challenging. My best advice is don’t overthink it. And to quote my adviser, "you just gotta do it." At the end of...
AAV Titers: Where Do They Come From And What Do They Mean?

Type

Blog Post

... Figure 2: Label that comes on Addgene’s AAV aliquots. Our titer values (circled in red) report physical...
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

Type

Blog Post

...referring to the evil side of a mystical "Force," this quote is equally applicable to many microscopy experiments...
March for Science

Type

Blog Post

...it happen. In a time when a top official can be quoted as saying “I’m not in the job of having evidence...

Showing: 61 - 80 of 110 results

Results per page:

Tips for Titering Your Lentiviral Preps

Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

Hot Plasmids and Viral Preps - March 2021

Hot Plasmids and Viral Preps - January 2021

Hot Plasmids - October 2020

Pooled Library Amplifications

When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

10 Basic tips for mammalian cell culture

Hot Plasmids and Viral Preps - May 2021

Which Fluorescent Protein Should I Use?

Droplet Digital PCR for AAV Quantitation

Simplify Cloning with in vivo Assembly

Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

An Inside Look into Addgene's Dev Team

Getting the Most from Your Lentiviral Transduction

How to Write a Scientific Review Article

AAV Titers: Where Do They Come From And What Do They Mean?

Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

March for Science

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