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We narrowed to 361 results for: reporter

Showing: 61 - 80 of 361 results

  1. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...Lab at MIT developed plasmid based systems that report the order in which specific chemical inputs are...recombinase-based state machines could potentially be used to report upon and/or engineer “temporally ordered biochemical...derived from Zika virus and, upon binding, produce reporter proteins. These toehold switches can be used to...viral replication and transcription as well as reporter genes. To learn how you can use minigenomes in...

  2. RUBY-Red Siliques

    Type
    Blog Post
    ...challenging and requires specialized equipment. Reporters for studying gene expression have similar drawbacks...added to the palette. RUBY is a bright red pigment reporter whose expresion is visible to the naked eye in... enzymes and a spontaneous step. (B) RUBY is a reporter consisting of these three enzymes expressed under...References  He, Y., Zhang, T., Sun, H. et al. A reporter for noninvasively monitoring gene expression and...

  3. TALEN Engineering

    Type
    Collection
    ...for Endogenous Human Genes TALENs for the EGFP Reporter Gene Additional Plasmids compatible with the REAL...

  5. Neurodegeneration Research Collection

    Type
    Collection
    ... S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian cells. Lathuiliere... (AIS) plasticity with a motor neuron-specific reporter and a PAX7 inducible vector . Harley et al. Cell...

  6. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ..., the MIT Technology Review dropped a bombshell report. A scientist working in China was using CRISPR/...While the Nuffield Council has recently published a report on the social and ethical issues of genome editing...question of “how do we move forward” is the June 2012 report on the ethics of “Novel techniques for the prevention...diseases. The key success of the Nuffield Council’s report is that when it became clear that this assistive...

  8. Hot Plasmids - May 2022

    Type
    Blog Post
    ... a cleavage free manner. The dCas9-SSAP system reports an efficiency of up to 20%, comparable to commonly... For a one kilobase insertion, the researchers reported an editing error rate of less than 0.3%, both ...using mStrawberry, localization peptides, and reporters that drive the expression of the fluorescent protein...response element (TRE) or kinase translocation reporters (KTRs). Find visual barcode plasmids here! Kaufman...

  9. Serotype Testing AAV

    Type
    Collection
    ...Serotype Testing AAV These AAV encode fluorescent reporters and can be used to compare the tropism of different...

  10. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...GCaMP6 family of sensors are commonly used calcium reporters with different kinetic parameters. There are three...also available. Detection methods Many calcium reporters use a single fluorescent protein (FP), requiring...unwanted variables. Twitch is a FRET-based calcium reporter derived from troponin C. It is engineered with...for in vivo studies) There are several calcium reporters specially designed for the aims and requirements...

  11. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    ...bailii) and B3 (from Zygosaccharomyces bisporus.) Reporter constructs equivalent to site-STOP-site-myrRFP...cross-reactivity of these recombinases, each recombinase and reporter construct pair were tested for excision efficiency...cross-reactivity; however, the other recombinase/reporter pairs were inactive, even at very high recombinase...

  12. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...background.” Just last month in Scientific Reports, Verkhusha’s team reported another advance in the application.... Transparency window That’s exactly what they reported doing in a paper that appeared in Nature Biotechnology...

  13. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...mild symptoms. This changed in January with the report of an outbreak of the virus in Brazil that was ...-ready format when the Zika virus outbreak was reported, and we felt that, given the critical need for...such a short timescale. Our article ultimately reported the development of a rapid design and prototyping...Find the Zika Sensor Constructs at Addgene As we report, we have embedded this next generation of toehold...

  14. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ... development of several fluorescent autophagy reporters now allows for the measurement of autophagic flux... al. "Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in...using a single fluorescent biosensor." Scientific reports 5 (2015): 12397. PubMed PMID: 26215030. PubMed ...

  15. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...mutagenesis libraries or to insert heterologous genes, reporters, or other markers into a target genome. They did...Rep 2014 Jun 27;4:5469. MitoTimer plasmids to report mitochondrial turnover Mitochondrial health...Gottlieb’s lab has used an inducible MitoTimer reporter using a Tet-on system (pTRE-Tight-MitoTimer) to... to show its usefulness in cell culture to report changes in mitochondrial turnover and transport. pMitoTimer... Yan’s lab, which is constitutively expressed, reports on the balance of biogenesis and mitochondrial ...channelrhodopsins. CnChR1, nicknamed Chrimson, is the first reported yellow-peaked channelrhodopsin with a spectral... original APEX (enhanced ascorbate peroxidase) reporter from Alice Ting’s lab has enabled progress on ...

  16. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...maintaining fluorescence. The protein structure, first reported in 1996, is an eleven β-sheet-containing “barrel...constructing fluorescent fusions. Transcription Reporter: Placing GFP under the control of a promoter of...a given cell type. This type of transcription reporting was among the earliest uses of GFP. Fluorescence...micrometastases in nude mice in cancer studies, act as a reporter for DNA double strand break repair, and to label...

  17. Synthetic Biology - Overview

    Type
    Collection
    ... modification, including shuttle, integration, reporter, and tagging vectors. Metabolism Browse plasmids...

  18. Science Career Options

    Type
    Blog Post
    ...actively require these skills, including: Science Reporter Scientific Sales Epidemiologist Scientific Recruitment... actively use these skills include: Science Reporter Scientific Illustrator Research and Development...desirable in a range of roles, including: Science Reporter Technical Writer Statistician Bioinformatician...

  19. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    ...tasked with was creating and sustaining a biweekly report of our social media analytics, something that had... meant I was responsible for establishing a new report and routine across the marketing and content team...a fantastic spreadsheet template to build out a report on. I was able to understand what metrics would...

  20. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ... cells and activate expression of a luciferase reporter. CPP was not required for protein entry into cells...primary cell types using Cre and various loxP reporters. In both mouse and human embryonic stem cells,...Plasmids @Addgene: iTOP-tested proteins and reporters Previous Protein Delivery Techniques: Introduction...
Showing: 61 - 80 of 361 results