We narrowed to 133 results for: tet

1. Hot Plasmids and Viral Preps - May 2021

   Type

   Blog Post

   ...where gRNA expression is induced by anhydrous tetracycline. Three new anti-CRISPRs were identified that...-mRuby from Liqun Luo’s lab expresses membrane tethered GFP to enhance axonal labeling and synaptophysin-tagged...

2. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   ...interactions that uses split fluorescent proteins tethered to potential protein interaction partners. Unlike... form the complete FP because the PIPs they’re tethered to bring them into close proximity (Figure 1)....

3. MXS Chaining

   Type

   Blog Post

   ... fluorescent protein were fused in-frame to a tethering partner, which directed localization of the fluorophore...Construct Fluorophore Excitation/emission maxima Tethering partner Subcellular localization 1 TagBFP 399nm...

4. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

   Type

   Blog Post

   ...while nuclease-deactivated Cas9 (dCas9) can be tethered to a repressor (CRISPRi) or an activation domain...RNA-guided DNA binding protein (dCas9). dCas9 is then tethered to a repressive domain, such as KRAB, to prevent...

5. Antibodies 101: Immunoprecipitation

   Type

   Blog Post

   ...antibody against a target protein is immobilized, or tethered, on agarose or magnetic beads and then incubated...antibody binds to the target protein, thereby tethering it to the beads. Unbound proteins in the mix are...

6. Hot Plasmids: Fall 2024

   Type

   Blog Post

   ...on an integrative plasmid containing tetracycline repressor TetR and resistance selection marker KanR....

7. CRISPR-based Genome Editing Systems For Editing in E. coli

   Type

   Blog Post

   ... different induction systems, such as PT7, Prha, Ptet, and Para. The pDonor plasmid pRE57-Ter with multiple... plasmids for multicopy integration. (B) Anhydrotetracycline (aTc) induces expression transposases leading...

8. Pouring LB Agar Plates

   Type

   Protocol

   .../mL 50 µg/mL Spectinomycin 50 mg/mL 50 µg/mL Tetracycline 10 mg/mL 10 µg/mL Notes: Unless otherwise indicated...

9. Antibody Validation Using the Indirect ELISA Method

   Type

   Protocol

   ...PBS, 1X pH 7.4, VWR 45000-446 TMB (3,3',5,5'-tetramethyl-[1,1'-biphenyl]-4,4'-diamine) substrate kit, ...

10. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type

    Blog Post

    ...BxbI gene downstream of the anhydrotetracycline (ATc)-inducible promoter (PLtetO), the TP901 gene downstream...

11. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type

    Blog Post

    ...system is to use a lambda red phage, λTetR, that carries the tetracycline resistance gene and the lambda red...

12. Custom CRISPR Screens & the Green Listed Software

    Type

    Blog Post

    ...CMM), Department of Medicine, Solna, Karolinska Institutet, in Sweden. It can be very time consuming to ...CMM), Department of Medicine, Solna, Karolinska Institutet, in Sweden. He has a particular interest in using...

13. Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering

    Type

    Blog Post

    ...CRISPR/Cas9 compared to ZFNs and TALENs,” said Tetsushi Sakuma of Hiroshima University. “However, there...

14. More Data for You: Find Articles Citing Addgene Plasmids

    Type

    Blog Post

    ...new feature in action, click here to explore the TetO-FUW-OKSM plasmid page. Or search for your favorite...

15. How Exercise Purges Stress and Keeps Depression at Bay

    Type

    Blog Post

    ...researchers led by Dr. Jorge Ruas of the Karolinska Institutet, the discovery really wasn’t what they’d expected...

16. Let There Be LITE Plasmids

    Type

    Blog Post

    ...chromophores flavin-adenine dinucleotide and methyltetrahydrofolate, which are universally abundant. Second...

17. What's New in CRISPR - December 2019

    Type

    Blog Post

    ...ribonucleoproteins for transient expression. They replaced the tetraloop of the sgRNA scaffold with a com aptamer which...

18. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type

    Blog Post

    ... the world from the far-eastern islands,” said Tetsushi Sakuma, a lecturer at Hiroshima University who...

19. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type

    Blog Post

    ... by DNMT3A or MQ1, and cytosine demethylation by Tet1. RNA Targeting: This page contains Cas13 constructs...

20. Sleeping Beauty Awakens for Genome Engineering

    Type

    Blog Post

    ...and one set of vectors for inducible expression (pSBtet derivatives). The constitutive and inducible expression...