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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 172 results for: tox

Showing: 61 - 80 of 172 results

  1. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...produce viral proteins and die, either through cytotoxicity or the immune response. The second strategy ...expression. In cell lines that can produce HIV-1 toxic proteins, CRISPR/Cas9 SAM caused apoptosis, indicating...

  2. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...problematic because excitation light can cause phototoxicity, autofluorescence, and perturb biological phenomena...the case of exciting cyan-excitable donors, phototoxicity. Another challenge to using FRET sensors comes...

  3. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ... overexpression of your gene and could lead to toxicity or trouble interpreting your results, while a ...low expression.  Finally, the risks of potential toxicity and/or off-target effects (e.g. recombinase-dependent...

  4. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...high 3D spatial resolution while also avoiding the toxic effects of high light doses to the cells. Thin static... its fast temporal resolution and decreased phototoxicity. Lightsheet designs that allow for multi-view...

  6. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...tagRFP Ebert pSAG1::CAS9-U6::sgUPRT 54467 Other/Toxoplasma gondii none, Q5 mutagenesis yes, cut S. pyogenes...pyogenes Sibley pU6-Universal 52694 Other/Toxoplasma gondii BsaI yes, cut S. pyogenes Lourido pRGE31 50929...

  7. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...penetrate 5 mm past the surface of skin and is less phototoxic. Using the FAST system, Ye’s lab could edit a...editing, reduce off-target activity, and prevent cytotoxic effects. For a more detailed review, check out...

  8. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...  acrIIA3   Listeria monocytogenes  pCSW65 N/A (Toxic)   None N/A   acrIIA4 Listeria monocytogenes  ...  acrIIA3 Streptococcus pyogenes  pCSW24  N/A (Toxic) pJH375  No    ...

  9. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...efficiency of viral infection. However, polybrene is toxic to some cell lines. In these cell lines, substitute... media 24 hours after infection. TIP: If viral toxicity is observed in your cell line, you may decrease...

  12. AAV Molecular Tools

    Type
    Collection
    ...Cre-dependent Cre-dependent expression of diphtheria toxin receptor fused to GFP for studying cell ablation...

  13. Microbiology Resources

    Type
    Collection
    ...Entamoeba histolytica Leishmania sp. Plasmodium sp. Toxoplasma gondii Trypanosoma sp. Plasmids for Viruses Species...

  16. Ras Pathway

    Type
    Collection
    ...homolog RAC RAC1 RAC2 RAC3 Ras-related C3 botulinum toxin substrate (rho family, small GTP binding protein...

  17. CRISPR Guide

    Type
    Collection
    ...can increase the risk of off-target cutting or cytotoxicity, but naturally-occurring Acr (Anti-CRISPR) proteins...including resistances to chemotherapy drugs and toxins, cell viability, and tumor metastasis. One of the...
Showing: 61 - 80 of 172 results