We narrowed to 99 results for: ubc

1. Hot Plasmids - October 2020

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   ...-wrmScarlet labeling proteins with different subcellular locations. Image from Goudeau et al., 2020. ...

2. Viral Vectors 101: Chemogenetics

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   ... where specific tissues, cell types, or even subcellular regions of a neuron need to be targeted. Targeting...

3. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   ...recombinase. Addgene has several great options: pLV hUbC-dCas9-T2A-GFP for removable dCas9 expression and...

4. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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   ...Engebrecht J, Trimmer JS (2019) A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

5. Tips for Titering Your Lentiviral Preps

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   ...sorting based on expression of a surface marker, subcloning, expansion, and confirmation. This procedure ...

6. Viral Vectors 101: The Retroviral Lifecycle

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   ...been developed into retroviral vectors and 2.) a subclass of retroviruses called the lentiviruses (like ...

7. Negative Can Be Positive: Open AAV Data with Addgene

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   ...and Share AAV Data with Addgene's New AAV Data HubCelebrate Open Data Day with Addgene’s AAV Data Hub!Data...

8. Neuronal labeling with Spaghetti Monster

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   ...structures GFP is limited in its ability to label subcellular structures in the CNS. GFP and other FPs have...

9. Save Time with Transient Plant Leaf Transformations

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   ...fused to a protein of interest to elucidate the subcellular localization of your protein of interest. Find...

10. Hot Plasmids - November 2023

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    ...Andrews, N.P., et al. (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

11. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    ... in vitro without antibiotic selection. In a subcutaneous cancer model, the researchers showed that after...

12. Getting the Most from Your Lentiviral Transduction

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    ...transductions. Cell lines should be routinely subcultured at densities that are low enough to prevent confluency...

13. Tips for Using FRET in Your Experiments

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    ... Constructs to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian...

14. Allen Institute for Brain Science AAV Enhancer Collection

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    ...2021). Enhancer viruses for combinatorial cell-subclass-specific labeling. Neuron , 109 (9), 1449–1449... P. (2021). Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex...

15. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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    ...bait protein can even target BirA* to specific subcellular locations, such as the nuclear envelope, allowing...

16. Hot Biosensors 2022: Year-End Roundup

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    ...temperature variations and heat transfer at the subcellular level requires a probe with fast kinetics and...

17. Plasmids 101: Common Lab E. coli Strains

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    ...only includes general lab strains designed for subcloning or protein expression. If you were to include...

18. Technologies Enabled by NanoLuc® Luciferase

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    ...multiple cellular events such as the dynamics of subcellular structures and gene expression. You must see ...

19. CRISPR 101: Epigenetics and Editing the Epigenome

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    ... as seen with other epigenome-editing tools. pLV hUbC-dCas9-MQ1(Q147L)-EGFP is also available for lentiviral...

20. Antibodies 101: Multiplex Immunofluorescence

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    ... J., & Trimmer, J. S. (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...