...proteins tagged with genes or peptides with known subcellular localization patterns. Useful for co-localization...Proteins Organelle Markers Fluorescent Proteins: Subcellular Localization More Fluorescent Protein Resources...Cytoskeletal Junctions Secretory Pathway Background The subcellular localization of a protein is often tied to its...There are a variety of methods for determining subcellular localization. One of the most common is to use...proteins that are targeted to various organelles or subcellular structures. You should fuse your protein of interest...The cell contains a variety of organelles and subcellular structures each with their own unique function...

... luciferase Christopher Vakoc 33307 Ubc.Luc.IRES.Puro Firefly Ubc Lentiviral expression of firefly luciferase...luciferase Reuben Shaw 48688 pUltra-Chili-Luc Firefly Ubc Lentiviral expression of firefly luciferase and dTomato...Brent Stringer 102320 FUW-RLuc-T2A-PuroR Renilla Ubc Lentiviral Expression of renilla luciferase Rudolf...luciferase Georg Weber 113450 pLenti NL NanoLuc® Ubc Lentiviral expression of Nanoluc with a N-terminal...

...Ultra-Exon1Q23-Myc-A HTT Myc UbC Huntington's Baoji Xu 110487 Ultra-Exon1Q145-Myc-A HTT Myc UbC Huntington's Baoji...pUltra-Exon1Q23-Myc-B HTT Myc UbC Huntington's Baoji Xu 110489 pUltra-Exon1Q145-Myc-B HTT Myc UbC Huntington's Baoji...FUGW-APPwt-T2A-mCherry APP mCherry UbC Alzheimer's Ronald Hart 190804 FUGW-APPSwe-T2A-mCherry APP mCherry UbC Alzheimer's Ronald...FUGW-APPSwe-T2A-Hygro APP UbC Alzheimer's Ronald Hart 190806 FUGW-APPIce-T2A-mCherry APP mCherry UbC Alzheimer's Ronald...APOE mCherry UbC Alzheimer's Ronald Hart 190808 FUGW-APOE-E3-T2A-mCherry APOE mCherry UbC Alzheimer's ...T2A-mCherry APOE mCherry UbC Alzheimer's Ronald Hart 190810 FUGW-APOE-E4-T2A-Puro APOE UbC Alzheimer's Ronald...pTet-O-APOE-E2-T2A-Puro APOE UbC Alzheimer's Ronald Hart 190812 pTet-O-APOE-E3-T2A-Puro APOE UbC Alzheimer's Ronald...

...CMV-CFP;UBC-Cre25nt Cre with a 25 nucleotide extracellular vesicle targeting sequence and CFP UBC Mammalian...

...(CAG promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono...(CAG promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) Off Patrick Aebischer and Didier ...1alpha promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono...hPGK promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono...hPGK promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) Off Patrick Aebischer and Didier ...hUbiquitin promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono...hPrion promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono...

...antibodies can be isotype-specific or even isotype subclass-specific. This specificity can be used to ensure...Isotype Description Configuration and Valency Subclasses IgA Prevalent antibody in secretions (e.g. tears...should never be stored below 4 °C. Certain isotype subclasses, such as IgG3, aggregate after thawing and therefore...different species of origin, isotypes, and isotype subclasses of a primary antibody, which becomes the limiting...? You may need to buy isotype-specific or even subclass-specific secondary antibodies to allow for differentiation...

...Addgene Blog: Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids Viral Service...blue/green) Imaging intraorganellar Ca(2+) at subcellular resolution using CEPIA. Nat Commun. 2014 Jun ...genetically encoded sensors for population and subcellular imaging of cAMP in vivo. Nat Methods. 2022 Nov...FRET-based cGMP biosensor SponGee: A Genetic Tool for Subcellular and Cell-Specific cGMP Manipulation. Cell Rep...for mitochondria or cytosol Hypoxia triggers subcellular compartmental redox signaling in vascular smooth... Ultrafast fluorescent voltage sensor ASAP2f Subcellular Imaging of Voltage and Calcium Signals Reveals...

...53190 pLV hUbC-Cas9-T2A-GFP : Co-expresses human optimized S. pyogenes Cas9 and GFP. 53191 pLV hUbC-dCas9-...human optimized S. pyogenes dCas9 and GFP. 53192 pLV hUbC-dCas9 VP64-T2A-GFP : Co-expresses human optimized...

...Protein Resources: Empty Backbones Biosensors Subcellular localization Optogenetics Background Förster ... Constructs to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian Expresses...

...distinct IgG subclass from the conventional mAb to facilitate multiplex labeling with subclass-specific secondary...References Andrews NP, et al. 2019. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

...Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Biosensors Optogenetics Background...

...Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics The lab of Michael ...

...that affect signalling pathways within cells. Subcellular Localization Determine where your protein of ...

...Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics Background In vivo ...

...species (e.g., human, watermelon, Streptomyces ) Subcellular compartment (chloroplastic, cytoplasmic, glyoxysomal...

...CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization Background Plasmids Protocols Background...

...GRF4-GIF1 for wheat and other monocots - Jorge Dubcovsky. Engineered biocontainable RNA virus vectors for...

...of fluorescent proteins, including Markers of Subcellular Localization, Optogenetics Plasmids, and Chemogenetics...

...expression of fluorescent TAL effectors to visualize subcellular positioning of target DNA sequences in living...

...expression; puro resistance Sabatini 14883 FUGW 3rd hUbC-driven EGFP; can be used for cDNA expression Baltimore...