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We narrowed to 322 results for: virus

Showing: 61 - 80 of 322 results

  1. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ... to just dive right in and start injecting your virus, a successful AAV experiment starts with validation...compositions of AAV capsids, which determine how a virus enters the cell. Different serotypes utilize a number... serotypes have broad tropism (the ability of a virus to enter a particular cell type), but there are ...begin testing, you’ll next need to decide how much virus to put into your system, which is controlled through...picked, since that can affect the spread of the virus.  A better way to define high titer, then, is this...recombinase-dependent vectors) increases with the amount of virus you add, so it is strongly recommended to optimize...(2018). An Alternate Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor. Journal...

  2. Important Considerations When Using AAVs

    Type
    Blog Post
    ...mutagenesis in humans (1) and AAVs are non-enveloped viruses, so they are resistant to alcohol-based disinfectants...freezing and thawing. Although more stable than other viruses, multiple freeze thaw cycles can still reduce AAV... structure (scAAV) (7). As single stranded DNA viruses, AAVs normally require second strand synthesis ...phenotype. Previous neural tracing methods used viruses that were potentially toxic and would result in...4. Castle, Michael J., et al. "Adeno-associated virus serotypes 1, 8, and 9 share conserved mechanisms...Self-complementary recombinant adeno-associated virus (scAAV) vectors promote efficient transduction independently...efficient transduction by recombinant adeno-associated virus vectors." Journal of virology 70.5 (1996): 3227-...

  3. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ...poorly understood,” says Qiu.   Qiu developed a dual virus system using both optogenetics and chemogenetics...neurons is in progress, she has validated the dual virus method to study CR neurons which are depicted here...Schematic diagram of the construction of the dual virus and the experimental approach. (B) CR-Cre mice demonstrate... response due to blue light stimulation of dual virus-expressing CR neurons. (C) Duration of painful responses...) Immunohistochemistry verification of the dual virus expression in CR neurons of the spinal cord. Created...

  4. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...plasmid which contains the proteins needed for the virus to replicate,  and the transfer plasmid which contains...Cre-Lox system.  WPRE The Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE...Bachle SM, Kamens J, Fan M (2019) Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation...the Inverted Terminal Repeats of Adeno-Associated Virus. Human Gene Therapy Methods 30:206–213 . https:/...

  5. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...engineering viruses Acr genes can be used as positive selectable markers in engineering viruses. For example...Editing of Sulfolobus islandicus Rod-Shaped Virus 2. Viruses 10:695 . https://doi.org/10.3390/v10120695...difficult-to-engineer Sulfolobus islandicus rod-shaped virus 2 (Mayo-Muñoz et al., 2018). Only viral particles...Papayannopoulou T, Lieber A (2018) HDAd5/35++ Adenovirus Vector Expressing Anti-CRISPR Peptides Decreases...

  6. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Transducing Cells Thaw the virus on ice. Prepare the following dilution series of virus in DMEM complete: Prepare...the first virus dilution, add 160 µL of viral stock to 240 µL of media. When adding the virus to the diluent...the following virus dilutions were used to transduce cells: Initial dilution factor of virus stock Dilution...titer, TU/mL V = Viruses per genome C = # Cells per well D = Dilution factor v = Virus volume, mL *In the...Titration by qPCR Addgene Viral Service Lentivirus Guide Virus Blog Posts Introduction This protocol describes...diluent, pipette 10 times, to remove virus from pipette tip. To mix the dilution, set the P200 pipette...as indicated in the chart below. Dilution Factor Virus Volume to Dilute Media Volume Total Volume 2.5 160...

  7. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...Adeno-associated viruses (AAV) are an important biological tool that allow scientists to express a gene...single stranded DNA genome of AAV in the native virus. Since the WT sequence is relatively unstable, a... L., & Samulski, R. J. (2020). Adeno-Associated Virus Serotype-Specific Inverted Terminal Repeat Sequence...the Inverted Terminal Repeats of Adeno-Associated Virus. Hum Gene Ther Methods, 30(6), 206–213. https://...Repeat Integrity on rAAV8 Production Using the Baculovirus/Sf9 Cells System. Hum Gene Ther Methods, 28(5...

  9. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...targeting human and mouse genes. Virus Protocols for titering and testing your virus preparations. Name Description...protocol Fluorescence Titering Assay for Lentivirus Quantify virus using fluorescence measurements Colony...Colony Formation Titering Assay for Lentivirus Quantify virus by counting antibiotic resistant colonies Generating...physical titer of your adeno-associated virus preparation Lentivirus Titration by ddPCR Use ddPCR to measure...Lab Bench Basic Molecular Biology Plasmid Cloning Virus Antibodies Intro to the Lab Bench Introductory techniques...Production in HEK293 Cells Produce adeno-associated virus to deliver your gene of to the target of your choice...Gradient Ultracentrifugation Purify adeno-associated virus from a preparation produced in mammalian cells Watch...

  12. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...advantages of viruses and naked DNA while eliminating some of the drawbacks. Specifically, viruses are able...certain forms of gene therapy - avoiding the use of viruses is also important for social and regulatory reasons...

  13. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    ... serve the scientific community. Here are a few virus-related protocol videos that may help to save you...produce vital tools in the lab such as antibodies or viruses. If you’re new to tissue culture, check out this...

  14. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...interest, for example, a sequence specific to a virus or pathogen. Once Cas13 recognizes and binds to ... distinguish between Zika and a closely-related virus, Dengue from multiple sample sources. SHERLOCK could...including clinical diagnostics (e.g. pathogen or virus detection), therapeutics and sensitive genotyping...specific DNA sequence, such as the Human papilloma virus (HPV) genome, via a crRNA. An ssDNA-fluorescently...Learn about using toehold switches to detect Zika virus   Learn more about targeting RNA with Cas13a (C2c2...

  15. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ... human lung cancer cell line), armed only with viruses obtained through Addgene’s viral service and the...repeatability and bad news for a screen. Because Addgene viruses are provided at a specific titer, you can better...expression was the most efficient for gene editing. A virus encoding GFP plus a gRNA targeting GFP (pXPR_011...activity in my stable A549 cell pools. Using this virus, it’s expected that Cas9 activity, guided by the... I just randomly chose one of my stable pools. Viruses containing BRAF gRNA (BRDN0000561167 and BRDN0001146266...regions of your gene. I used existing BRAF gRNA viruses, but you can find a list of validated gRNA plasmids...Lines with Lentivirus Isolating a Monoclonal Cell Population by Limiting Dilution Lentivirus Production...

  16. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...the purification step because they are inside the virus itself. While these impurities are generally considered...Prokaryotic Sequences during Recombinant Adeno-Associated Virus Production and Their in Vivo Persistence after Vector... in rAAV Vector Preparations by Single-stranded Virus Next-generation Sequencing. Molecular Therapy - ...Control Testing of Recombinant Adeno-Associated Viruses. In: Methods in Molecular Biology. Humana Press....org Find Ready-to-Use AAV Find Ready-to-Use Lentivirus Browse Viral Plasmids ...

  17. Colony Formation Titering Assay

    Type
    Protocol
    ...Formation Titering Assay for Lentivirus You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols...the seeding of the cells at the same time as the virus-mediated transduction. For some cell lines, transduction...into 20 mL of media. If using freshly collected virus, filter through a 0.45 μm polyethersulfone filter...freeze-thaw. If you are freezing and aliquoting virus, it is recommended that you titer from the frozen...titer associated with freeze-thaw. If using frozen virus, thaw the lentiviral aliquot on ice prior to use...Complete Containing 10 μg/mL Polybrene (μL) Volume of Virus Added to Plate (μL) Volume of Cells Added to Plate...Plate (μL) Final Viral Dilution 1:10 100 of Stock Virus 900 150 1,350 1:100 1:100 100 of 1:10 900 150 1,...

  19. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...adaptive defense strategy against life-threatening viruses. Evolving to survive Although the CRISPR sequences...ticket to ensure prokaryotic survival; however, viruses, too, are famous for their ability to devise new...strategies to reproduce within a host cell. A few virus particles have learned to circumvent the CRISPR/...

  20. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...viral vector systems based on retroviruses like Moloney murine leukemia virus (MMLV). The vectors could integrate...Resources on Addgene.org Check out Our Lentivirus Guide Learn about Virus Biosafety Find Lentiviral Vectors...possibility of creating a replication-competent virus via recombination between the transfer and packaging...events necessary to create a replication-competent virus. Second, Tat is eliminated through the addition ...useful viral vectors in the lab. Advantages of lentivirus include a large genetic capacity and the ability...dividing cells. Another type of vector, based on adenovirus, could infect non-dividing cells, but without... Didier Trono and collaborators turned to the lentivirus HIV-1, well-known to infect non-dividing cells...
Showing: 61 - 80 of 322 results