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...for rabies virus-based monosynaptic tracing. These AAV can be used with modified rabies virus to perform...that lack the G protein, the virus can replicate but the newly formed viruses are unable to infect any neighboring...envelope protein of (EnvA) of leukosis virus. EnvA can direct virus infection specifically into cells that...neuronal tracing, to be used with modified rabies virus. Viral...tracing. Note : Addgene does not distribute rabies virus. AAV Vectors for Monosynaptic Neuronal Tracing ID...Can be used to complement deletion-mutant rabies virus AAV1 Ian Wickersham 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA...can be used to complement deletion-mutant rabies virus AAV1 Ian Wickersham 100799 pAAV-TREtight-mTagBFP2...

...components of one virus (such as HIV, murine leukemia virus, and vesicular stomatitis virus G) are packaged...the study of the live virus. Alternatively, it is possible to generate pseudovirus, where the structural...suitable for generating pseudotyped virus for SARS-CoV-2 and other coronaviruses using appropriate packaging ... Protein Nemazee lab - Pseudotyped virus protocol for coronaviruses (DOCX, 184 KB) Addgene Blog Posts ...-19 – Viral Pseudotyping SARS-CoV-2 Pseudotyped Virus SARS-CoV-2 Spike Plasmids Accessory Plasmids Other...packaged as replication-defective virus particles with the SARS-CoV-2 spike (S) glycoprotein on the envelope...envelope surface. Pseudotyped virus particles can then be used to infect permissive cells that express ACE2...

...-type virus propagation and use of viral plasmids to produce viral vectors. A) Wild-type viruses infect...BioRender.com. Wild-type viruses have one goal, which is to make more viruses. The viral genome contains...the host’s machinery to produce more viruses. In this way, viruses are excellent vehicles to deliver genetic...propagate in the same way that wild-type viruses do. For general virus biosafety guidelines, check out Addgene's...transduce dividing and non-dividing cells. These virusal vectors enable long-term stable gene expression...Vector Type Expression Genome Packaging Capacity Virus Size Cells Infected Host Genome Integration Immune...the necessary genes to achieve this goal. When a virus recognizes a host cell, it injects its viral genome...

...Description PI 16402 pShuttle For production of viruses containing transgene under a chosen promoter Vogelstein...Vogelstein 16403 pShuttle-CMV For production of viruses containing transgene under CMV promoter Vogelstein...16404 pAdTrack For production of GFP-trackable viruses containing transgene under a chosen promoter Vogelstein...16405 pAdTrack-CMV For production of GFP-trackable viruses containing transgene under CMV promoter Vogelstein...50957 RedTrackCMV For production of mRFP-trackable viruses containing transgene under CMV Bamburg 16407 pAdEasy...cassette Zhang 50958 ShuttleNSE For production of viruses containing transgene under Neuron-specific enolase...62621 pShuttle-CMV-F2A-T2A-Venus For production of viruses with multicistronic expression and a Venus tag ...

...Plasmids for Viruses Species Dengue Hepatitis Influenza Mumps virus Rubeola (measles) West Nile virus (WNV) ... your microbe of interest, including bacteria, viruses, protozoa, fungi, and more. Plasmid...range of microbial fields, including bacteria, viruses, parasites such as protozoa, and fungi. Find plasmids...complementation constructs - Lahaye Lab Vaccinia virus ORF library : Expression library that contains each... the predicted open reading frames for vaccinia virus - Bennink and Yewdell Labs Yarrowia lipolytica Golden...

... specific virus in order to generate high quality preparations. After production, all virus preps are ...Resources Lentivirus Production Titer Quality Control Resources Overview of Viral Production Viruses are generated...experiment using our virus protocols . Browse our AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral...preps available from Addgene. After receiving your virus from Addgene, read our detailed recipient instructions...contamination. In the event of contamination, all of the virus produced in the line will be taken off-line and ...preps available from Addgene After receiving your virus from Addgene, read our detailed recipient instructions...instructions for next steps. Plan an experiment using our virus protocols . Browse our plasmid collection of popular...

... Institute The Coronavirus Disease-2019 (COVID-19) pandemic was caused by a novel virus strain from the... Protein Nemazee lab - Pseudotyped virus protocol for coronaviruses (DOCX, 184 KB) Research Articles Many...elucidate as much as they could about this novel virus. SARS-CoV-2 entry into a host cell,...SARS-CoV-2 spike pseudotyped virus. It also lists several luciferase and fluorescent... protein constructs for making VSV pseudo virus. Addgene blog Scientists Map the SARS-CoV-2-Human...mediating the entry of SARS-CoV and SARS-CoV-2 viruses (Anderson et al., 2020). TMPRSS2 plasmids - a serine...primes the SARS-CoV-2 S protein and is involved in virus entry into cells (Hoffman et al., 2020). FURIN plasmids...

...100798-AAV1 pAAV-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing; to be coinjected with ... 100799-AAV1 pAAV-TREtight-mTagBFP2-B19G helper virus for monosynaptic tracing; to be coinjected with ...millisecond resolution optogenetics. Cre-dependent virus Edward Boyden 108422-AAV5 pAAV-CAG-FLEX-Archon1-...pAAV-hSyn-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under synapsin promoter...pAAV-CAG-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter ...pAAV-hSyn-dLight1.2 To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoter...

...that was used to make the virus. For more details, see our FAQ on What is virus associated DNA, and why ...Cart *Each viral service request will also include virus associated DNA (vdna), which is a sample of the ...vector packaging service, but lets us know what viruses would be helpful to the scientific community....

...and useful types of viruses used in research. Lentivirus can transduce both dividing and non-dividing ...cells without a significant immune response. These viruses also integrate stably into the host genome, enabling...Plasmids Envelope Plasmids Resources Lentiviruses, a subset of retroviruses, are some of the most common and...enabling long-term transgene expression. Wild-type lentiviruses have been engineered into viral vectors for ...David Root 44012 pInducer20 3rd Tet-inducible lentivirus for ORF expression, multicistronic design enables...psPAX2-D64V 2nd For making integrase-deficient lentivirus. David Rawlings and Andrew Scharenberg 22502 ...

...Plasmids Additional Resources Gamma-retroviruses are a subtype of retrovirus and contain a viral genome encoded...into a variety of cell types. Wild-type gamma-retroviruses have been engineered into gamma-retroviral vectors... vectors for safe use in the lab. The gamma-retrovirus components necessary to produce infectious viral...

... AAV viruses require the presence of a "helper" virus, such as adenovirus or herpes simplex virus, to ...the use of these viruses during recombinant AAV vector production, necessary helper virus genes are instead...

...catalog items. Virus-associated DNA Each viral vector service request will also include virus-associated ... the original plasmid that was used to make the virus. In order for Addgene to provide viral vector services... libraries. Viral preparation options include lentivirus and AAV. Viral...immediately after MTA approval. Browse AAV Browse Lentivirus Packaged on Request Choose from 30,000+ options...

...Ready-to-use adeno-associated virus (AAV) available from Addgene's viral vectory packaging service. Browse...Service AAV Browse In-Stock AAV Adeno-associated virus (AAV) vectors are safe and useful gene delivery ...Ablation, CRISPR Monosynaptic Neuronal Tracing Rabies Virus-Based Serotype Serotype Testing Samples Small samples...

..., Cre-dependent For generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on..., Cre payload. Must use with AAV8-DIO-Reporter viruses infected in postsynaptic cells. 5, 8 Don Arnold... Flp payload. Must use with AAV8-fDIO-Reporter viruses infected in postsynaptic cells. 8 Don Arnold 232353...vector packaging service, but lets us know what viruses would be helpful to the scientific community....

...165510 pOpen-DNA Topoisomerase 1B Vaccinia Virus Vaccinia Virus DNA Topoisomerase 1B 165515 pOpen-EcoRIM...165546 pOpen-MMLV_RT (mut H) Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated... pOpen-MMLV_RT (lack H) Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH...

...Bogner, et al. 2015. Capsid Mutated Adeno-Associated Virus Delivered to the Anterior Chamber Results in Efficient...rAAV2tYF-CB-hRS1, a Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin. Hum Gene Ther Clin...Transduction of Retina by Recombinant Adeno-Associated Virus Vectors. J Virol . Mar 28;90(8):4215-4231. PMID:...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...

... controls Puromycin Doench , Root Cas9 Lentivirus Cas9 viruses can be used to interrogate the genome in...fluorescence. Morris Control Lentivirus Browse our list of available viruses based on plasmids in our repository...guarantee viral service, but lets us know what viruses would be helpful to the scientific community.... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently...Pooled Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that are currently in ... viral preparation from the inventory below. Lentiviruses are distributed as purified viral particles ...

...targeting strategy that allows adeno-associated virus (AAV)-borne payloads to be expressed in cells based...recombinase-expressing animal lines and retro-grade expressing viruses delivering additional recombinases 10–14 . Figure...principles for working with INTRSECT recombinases, viruses, and important controls to consider as part of ...

...Arabidopsis mutants. Engineered biocontainable RNA virus vectors for non-transgenic genome editing across... improved vector system based on tobacco rattle virus (TRV) that simplifies gene silencing and genome ...