We narrowed to 147 results for: yeast
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TypeCollection...Addgene Kits MoClo-YTK Yeast Gateway Kit Multiplex Yeast Toolkit (MYT) Markerless Yeast Localization and Overexpression... Depositor Collections Botman-Teusink Yeast FP Collection Botman-Teusink Yeast FP Collection... . FP Tagging Yeast tagging vectors to create fusions of proteins of interest with yeast codon-optimized...proteins in yeast. Depositor...Overexpression Resources This comprehensive collection of yeast codon-optimized fluorescent proteins (FPs) in a ...FPs and tagging of genes of interest with FPs in yeast. Characterization of some of the FPs in this collection...constitutive overexpression of codon-optimized FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et...
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Plasmids 101: Inducible Promoters
TypeBlog Post..., the various eukaryotic cell types (mammalian, yeast, plants, etc.) require unique promoters and there... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post...a transcription activation system co-opted from yeast (1). It is a Drosophila geneticist’s main workhorse... -
Plasmids 101: Blue-white Screening
TypeBlog Post...Post on Common Lab E. coli Strains Learn to Use Yeast Two Hybrid Systems Browse All of Our Plasmids 101... -
Plasmids 101: Mammalian Vectors
TypeBlog Post...genomes. Unlike plasmids used for expression in yeast and bacteria, plasmids used for stable transfections... -
Synthetic Biology - Yeast
TypeCollection... Plasmid Collections Synthetic Biology Yeast Synthetic Biology: Yeast SynBio Resources...collection of synthetic biology plasmids for use in yeast. Yeast Plasmids Search the table by keyword or sort...plasmids for use in yeast. Plasmid... -
Microbiology Resources
TypeCollection... and yeast expression vectors : FX cloning System - Geertsma and Dutzler Labs EasyClone 2.0 Yeast Toolkit...transcription units in the oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors for ...and biosafety regulations. Looking for E. coli or yeast plasmids? Addgene has a wide variety of tools for...-gene constructs for S. cerevisiae - Dueber Lab Yeast Prototrophy : Restoration of prototrophy in common...depositor protocols and gRNA design software. Addgene yeast CRISPR resources Bacterial CRISPR Resources The ...Link opens in a new window) National Collection of Yeast Cultures (NCYC) (Link opens in a new window) NIH... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...Gate Assembly. They built this toolkit upon the Yeast Toolkit by replacing the backbone vector’s high-... -
Plasmids 101: Screens vs. Selections
TypeBlog Post...assayable with CRISPR. Another method, popularized in yeast, are temperature sensitive screens. These involve... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...techniques being developed. In the mid-aughts, the first yeast GCE paper was published, followed soon by GCE done... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post... for use in model organisms such as E. coli and yeast. Because this approach uses standardized parts, ... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...brightness can be made. This strategy has been used in yeast by Lee et al (2013) and in nematodes by El Mouridi... -
Modular Cloning Guide
TypeGuide...biosensors in yeast (requires MoClo-YTK parts for use). Yeast GoldenBraid Cloning System and Toolkit Yeast Expression...for S. cerevisiae expression. Multiplex Yeast Toolkit (MYT) Yeast Expression, CRISPR Tom Ellis 96 plasmids...Aspergillus species. MoClo Yeast Secretion and Display (YSD) Toolkit Yeast Expression Paul Young 34 plasmids...surface display of proteins of interest. Yeast Secrete and Detect Yeast Expression Sylvestre Marillonnet Plasmids...secretion in yeasts S. cerevisiae and P. pastoris (a.k.a. K. phaffii ). POMBOX MoClo YTK Yeast Expression...transcription units in the oleaginous yeast Y. lipolytica . EXPRESSYALI Yeast Expression Irina Borodina Plasmids...into Y. lipolytica chromosomal loci. Yeast GPCR-sensor Toolkit Yeast Expression Tom Ellis Plasmids for constructing... -
Fluorescent Protein Guide: Empty Backbones
TypeCollection...Expression pFA6a-PA-GFP-kanMX6 - Yeast Expression pFA6a-link-yEPAGFP-CaUra3 - Yeast Expression PATagRFP 562 595...8NeGFP - Zebrafish/Xenopus/Worm/Sea urchin pKT0128 - Yeast Expression EGFP-pBAD - Bacterial Expression mEGFP...-mBaoJin - Bacterial Expression P415 ymBaojin - Yeast Expression mGreenLantern 503 514 73 5.6 14 min Monomer...- Mammalian AAV Expression pBAD-mGreenLantern - Yeast Expression Clover 505 515 84 6.1 30 min Prone to... Mammalian Expression pFA6a-link-yoClover-Kan - Yeast Expression dClover2 505 515 Dimer (A206K) dClover2...Expression mVenus C1 - Mammalian Expression pKT0090 - Yeast Expression mVenus-pBAD - Bacterial Expression SYFP2...Expression mGold 515 530 68 5.9 Monomer pJL-mGold - Yeast Expression pCaggs-mGold - Mammalian Expression mGold2s... -
Plasmids 101: Modular Cloning
TypeBlog Post...strategies are not available to those working with yeast or plants. Using MoClo, or better yet, a pre-made... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post...Run) and to detect protein-protein interactions (yeast-two hybrid, Co-IP, BioID), and that’s just to name... -
CRISPR Plasmids - Yeast
TypeCollection... CRISPR Yeast CRISPR Plasmids: Yeast Browse CRISPR Plasmids By Function...have been designed for use in yeast. CRISPR... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...following CRISPR plasmids have been designed for use in yeast. Cut Fully functional CRISPR/Cas enzymes will introduce... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...Basta Kim pMZ283 52224 Yeast CspCI none S. pyogenes URA4 Zaratiegui pJZC588 62315 Yeast none S. pyogenes URA3...gRNA_handle_RPR1t 62966 Yeast HindIII none S. pyogenes Puro Lu pML104 67638 Yeast BclI-SwaI yes, cut S. ...Wyrick pML107 67639 Yeast BclI-SwaI yes, cut S. pyogenes LEU2 Wyrick pT040 67640 Yeast BclI-SwaI none S....Jackson pMZ376 74213 Yeast rrk1 yes, cut S. pyogenes KanMX Zaratiegui pMZ377 74214 Yeast rrk1 yes, cut S. ... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...Mammalian, Lentiviral, AAV, Bacteria, Drosophila, Yeast, Plant, Xenopus, Worm, or Other. Whether the plasmid... S. pyogenes Yang pRPR1_gRNA_handle_RPR1t 49014 Yeast HindIII none S. pyogenes LEU2 Lu pDestTol2CG2-U6... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog Post...constructed plasmids can be used to replace genes in yeast, mice, or Drosophila through homologous recombination... -
Plasmids 101: Terminators and PolyA signals
TypeBlog Post.... Additional Resources A detailed analysis of yeast terminators of varying strength. A discussion of...