We narrowed to 152 results for: yeast

1. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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   ...a transcription activation system co-opted from yeast (1). It is a Drosophila geneticist’s main workhorse...

2. Plasmids 101: Blue-white Screening

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   ...Post on Common Lab E. coli Strains Learn to Use Yeast Two Hybrid Systems Browse All of Our Plasmids 101...

3. Plasmids 101: Mammalian Vectors

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   ...genomes. Unlike plasmids used for expression in yeast and bacteria, plasmids used for stable transfections...

4. GCE4All: Making Genetic Code Expansion Accessible

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   ...techniques being developed. In the mid-aughts, the first yeast GCE paper was published, followed soon by GCE done...

5. Plasmids 101: Screens vs. Selections

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   ...assayable with CRISPR. Another method, popularized in yeast, are temperature sensitive screens. These involve...

6. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   ... for use in model organisms such as E. coli and yeast. Because this approach uses standardized parts, ...

7. Plasmid Tools for Microbiome Studies

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   ...Gate Assembly. They built this toolkit upon the Yeast Toolkit by replacing the backbone vector’s high-...

8. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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   ...brightness can be made. This strategy has been used in yeast by Lee et al (2013) and in nematodes by El Mouridi...

9. Cloning

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   ...protocol ) Back to Top Yeast-mediated Cloning and Oligonucleotide Stitching Yeast-mediated cloning is very...advantage of the powerful recombination abilities of yeast. Similar to Gibson, this method can efficiently ...accomplish this, you just need to introduce into the yeast the two (or more) fragments of DNA that you would...be set up to grow, transform and purify DNA from yeast. Back to Top...

10. RaPID Detection of RNA-protein Interactions

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    ...Run) and to detect protein-protein interactions (yeast-two hybrid, Co-IP, BioID),  and that’s just to name...

11. Plasmids 101: Modular Cloning

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    ...strategies are not available to those working with yeast or plants. Using MoClo, or better yet, a pre-made...

12. Plasmids 101: Knockout/Knock-In Plasmids

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    ...constructed plasmids can be used to replace genes in yeast, mice, or Drosophila through homologous recombination...

13. Plasmids 101: Terminators and PolyA signals

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    .... Additional Resources A detailed analysis of yeast terminators of varying strength. A discussion of...

14. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    ...efficiently and reversibly bias inheritance in wild yeast. bioRxiv 013896 (2015). doi:10.1101/013896. bioRxiv...methods are easy to implement and have been tested in yeast5. Ecological confinement involves performing experiments...

15. Botman-Teusink Yeast FP Collection

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    ...Resources Kits MoClo-YTK Yeast Gateway Kit Multiplex Yeast Toolkit (MYT) Markerless Yeast Localization and Overexpression... Depositor Collections Botman-Teusink Yeast FP Collection Botman-Teusink Yeast FP Collection... . FP Tagging Yeast tagging vectors to create fusions of proteins of interest with yeast codon-optimized...proteins in yeast. Depositor...Overexpression Resources This comprehensive collection of yeast codon-optimized fluorescent proteins (FPs) in a ...FPs and tagging of genes of interest with FPs in yeast. Characterization of some of the FPs in this collection...constitutive overexpression of codon-optimized FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et...

16. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    ...comparison to other display systems using phage and yeast (109-1010 members). This selection step involves...

17. 10 Basic tips for mammalian cell culture

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    ...microscope will help you identify bacterial and yeast contaminants. Set up a routine to split cells Don...

18. Plasmids 101: Degron Tags

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    ...It does work in mammalian cells, mice, and even yeast, though (we have a kit!). Choosing a degron tag ...

19. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    ... For subcellular markers in yeast see our plasmid collection from Sue Jaspersen (Stowers...

20. Synthetic Biology - Yeast

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    ... Plasmid Collections Synthetic Biology Yeast Synthetic Biology: Yeast SynBio Resources...collection of synthetic biology plasmids for use in yeast. Yeast Plasmids Search the table by keyword or sort...plasmids for use in yeast. Plasmid...