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We narrowed to 1,046 results for: ins;

Showing: 391 - 400 of 1046 results
  1. All in a Twist: dsRNA

    Type
    Blog Post
    ...target pathogenic proteins, as well as in the lab to deplete expression of proteins of interest. RNAi ...found (typically RNA that the RNAi is designed against), RISC can repress translation and/or degrade target...agriculture as species-specific pesticides and insecticides, an advancement over previous chemical versions... these structures can in turn lead to genome instability. Thus, it’s important to strike a balance between..., I. X., Grunseich, C., Fox, J., et al., Human proteins that interact with RNA/DNA hybrids. Genome Res...10.1101/gr.237362.118 Chen, G. Y., Hur, S. Cellular origins of dsRNA, their recognition and consequences. Nat...
  2. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...Genetically encoded voltage indicators (GEVIs) are proteins that detect and output the voltage changes in ...green-fluorescent GEVIs and blue-light-sensitive channelrhodopsins. Find the Ace-mScarlet voltage indicator plasmids...of the MoClo system in cyanobacteria. This kit contains 96 parts including terminators and promoters, ...half with PhyB or PIF6, phytochrome-like fusion proteins from the plant Arabidopsis thaliana. In the presence... light or PCB is no longer present, the fusion proteins separate, inactivating Cre. This is critical for...this system, cells express various fluorescent proteins controlled by Cre recombination. Each cell can...colors depending on the combination of fluorescent proteins expressed. CreLite-mediated recombination was ...
  3. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...funding from a variety of government and academic institutions. New graduate students may assume that their...given problem, Empowers the writer, And offers insight into a career as a Professor. Step 1: Your ... to read and fully understand the RFA. The RFA contains all of the important information required for ...the mission of the application and the funding institution. Moreover, avoidance of ‘to be’ verbs creates...time to review the literature and offer educated insight into what the desirable features of future technologies... with the description of the RFA and funding institution. Emphasizing how your solution fits into the ...funded proposal typically lists the deliverables, explains 1-2 potential pitfalls of the intended protocol...
  4. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...abilities, we’re gaining new ground in the fight against cancer in its many forms. The dichotomous interactions...major histocompatibility complex (MHC) class I proteins on their surfaces to avoid recognition by CD8+...formation and metastasis, block the cell-surface proteins that help escape immuno-surveillance, and curb...Antibodies that block cell-surface tolerance-producing proteins are one of the most commonly used immunotherapeutic...sequence of immune reactions that aid in the fight against cancer. Sipuleucel-T by the Dendreon Corporation...genome engineering have played a huge role. For instance, researchers are genetically altering tumor cells...cells and immune cells to knockout proteins involved in immune tolerance. Recently, China spearheaded ...
  5. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...resistance against Fusarium wilt disease in bananas as a Marie Curie Fellow at The Sainsbury Laboratory...blogger Sarah Schmidt, a Marie Curie Fellow at The Sainsbury Laboratory. Are you a science student or early...audience’s applause with your performance. When your institute opens its doors to the public, for example at ...your own blog, you can probably write for your institute’s blog. The Wellcome Trust blog or the John Innes... careers are in advance of yours, but also get inspired and meet peers. Join societies like National Science...provides on-site courses in public engagement for institutions and the Royal Society gives training in media...
  6. A Look at Addgene's QC Process

    Type
    Blog Post
    ...ensuring that the plasmid they ordered contains the correct inserts, tags, promoters, and other critical...markers, and fusion proteins listed for each plasmid  If the plasmids pass all inspection points, they are...ensure our assembly result is accurate The gene/insert or any major feature highlighted by the deposited...called Geneious. Here, the raw reads are mapped against a reference sequence, if one was provided. If no...assembly in Geneious can still provide additional insights to resolve the assembly and determine if there...
  7. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    ...pursue leadership opportunities at the local, institutional, regional, and national levels. These skills... But how does one gain such experience? Your institution may offer occasional workshops on leadership ...Harvard Medical School and then Boston University (institutions where I’ve worked as a postdoc). I soon realized...that many of the issues faced by postdocs and institutions are not local but are national issues. I applied...requirements of the new Board Members. In some instances I felt like I was going back to previous formats...every year it organized a “Committee Leadership Institute” (CLI) where each Committee Chair and Vice Chairs...the Burroughs Wellcome Trust. These “leadership institute’ weekends would entail intense training to develop...
  8. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...doing this with very little money. So when did inspiration strike us so hard that we upended our lives and...in fact, our color changing flower project was inspired by a Facebook comment.  In our day job, we worked...detector plants, we expected to see that same anger. Instead, social media comments were more often along the...that.” That was interesting. If people could be against GMOs as food but support GMOs that result in a ...business training. The money is a drop in the bucket against the funds needed to build a flower that changes...business enterprises, he can be found climbing mountains or playing with his two cats. Keira Havens grew...Biophysics and Biochemistry from the Illinois Institute of Technology in 2004, she accepted a commission...
  9. Transferable Skills: Negotiation

    Type
    Blog Post
    ...something done not by people wearing a lab coat, but instead by people wearing business suits discussing multimillion...that means you don’t. In this mindset, one of us wins, the other loses. Although this approach to negotiation...winner and a loser when negotiating, what if we instead approached it as a creative problem-solving exercise...point you toward a creative solution where everyone wins. What are the underlying reasons for this person...some data for an upcoming conference deadline. Instead of arguing over who gets the spot (a zero sum result...Coming up with creative solutions where everyone wins can initially feel more difficult because of the...can I offer them to make it a win-win situation instead of just extra work for them? As a business development...
  10. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...stem from the heart beating at an irregular or insufficient level. The heartbeat – a muscle contraction ...structurally into 2 main groups: voltage sensitive domains and Rhodopsin containing GEVIs. Chemigenetic sensors...that of the VSD GEVIs. However, since rhodopsin proteins were initially evolved for ion transport, not ...membrane due to the addition of several targeting domains. The quencher and voltage detector in this system...viruses also have various serotypes – the surface proteins that confer affinity for a target – and this serotype...pseudotyping is also a powerful tool that swaps capsid proteins from different viruses to optimize them for targeting... Viral Vectors 101: Optogenetics Fluorescent Proteins 101: Introduction to FRET ...
Showing: 391 - 400 of 1046 results