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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 838 results for: ANG

Showing: 781 - 800 of 838 results

  1. AAV ddPCR Titration

    Type
    Protocol
    ...outlined in this protocol are based on an AAV titer range of 5E+12–5E+13 GC/mL, where GC is genome copies ...slowly to avoid generating aerosols. Be sure to change tips each time. Dilution 1 (20X): 5 µL in 95 µL...directly in the center of the well and tilt to a 45° angle. Count to 20 while slowly and gently aspirating ... of the well and tilt the pipette tips at a 45° angle. Count to 20 while slowly and gently dispensing ...

  9. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type
    Protocol
    ... a short stretch of DNA to a plasmid, such as: Changing a Multiple Cloning Site (MCS) Adding short tags...once annealed can be cloned directly into the overhangs generated by restriction digest of existing sites...to include additional bases to complement the overhangs generated when digesting the vector with EcoRI...

  10. Centrifugation

    Type
    Protocol
    ... tubes. Depending on the centrifuge their speed ranges can vary, but are typically lower than some other...unbalanced state can damage the centrifuge and be dangerous for the user. If you do not have a balanced number...are not the same units and are not directly interchangeable. If you need to convert between the two, use...

  11. Antibodies 101: Validation

    Type
    Blog Post
    ...non-specific, background signal, so you’ll need a range of expression strengths to be confident in your ...

  15. Gibson Assembly Protocol

    Type
    Protocol
    ...two-part Gibson reaction if you're only making a small change in a plasmid (such as point mutations). Generate...T5 Exonuclease - creates single-strand DNA 3’ overhangs by chewing back from the DNA 5’ end. Complementary...England Biolabs by TelesisBio. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO. (2009). ...

  16. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ... a single stranded overhang on the digested end of the DNA fragment. The overhangs, called "sticky ends...sticky ends are compatible, meaning that the overhanging base pairs on the vector and insert are complementary...

  18. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...red were calculated using this plasmid, but will change if you use a different plasmid. Sample Calculation...dilutions 5–8 Note: at Addgene, samples typically range from 1 x 10 12 GC/mL to >2 x 10 13 GC/mL and we ...) ~ 1.0, E (efficiency of PCR) ~100% (90%–110% range is acceptable) Baseline removal: all samples will...

  19. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...drop down menu and select Scatter chart. Change the data range and select the cells where the x-axis is...Open . Choose the location of the file to open. Change the image type to 8-bit . Select Image . Select...

  20. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...the medium is acidified (i.e., the medium appears orange/yellow if using a standard phenol red pH indicator...control. 1 lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid #52962 ) and is described in Improved...libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nature Methods. 2014 Aug;11(8):783-4. (Link opens...
Showing: 781 - 800 of 838 results