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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,085 results for: ANT

Showing: 781 - 800 of 1085 results

  1. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...comparable (within r2=0.96) to that of NGS data. Advantages of ICE Determine Editing Efficiency and Types...  ICE uses Sanger sequencing data to produce quantitative, NGS-quality analysis of CRISPR editing - enabling...guide design tool, guide validation tool, and guaranteed editing with the CRISPR Gene Knockout Kit. Please...featured topic page xCas9: Engineering a CRISPR Variant with Pam Flexibility RNA Editing with Cas13 and...

  2. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...into AAV. NmeCas9 has the added advantage that it can be turned off by anti-CRISPR proteins. The Sontheimer...genome modification, but they did so without a substantial immune response or toxicity. More AAV-based CRISPR...Resources on the Addgene Blog Learn more about Cas9 Variants and the Expanded CRISPR Toolbox Read about CRISPR...

  3. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    ... allows access to a larger number of willing participants. While fibroblasts obtained from aged patients...hiPSCs than age-matched fibroblasts [3]. These advantages make PBMCs the ideal source of somatic cells ... vectors are based on the Epstein-Barr Nuclear Antigen-1 (oriP-EBNA-1) system where each vector contains...OCT3/4, SOX2, KLF4, C-MYC, LIN28 and SV40 large T antigen (SV40LT). The method had a reprogramming efficiency...

  4. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...These differences in recombinase efficiency are important to keep in mind when deciding whether to use the...common means to achieve ligand control is to fuse a mutant estrogen receptor (ER) ligand binding domain (LDB...recombinase. The high concentration of FLEx vectors guarantees that most cells are transfected with all labeling...versions can be used in these experiments. This elegant combination of Cre, FLP, and inducible systems ...

  5. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...that asbestos exposure remains the single-most important factor in developing mesothelioma. Asbestos, and...cells that can begin an immune response. This important biomarker can be used to distinguish between three...earlier, thus improving the patient’s prognosis significantly, but also to monitor those who may be at greater...be Used to Study Cancer Drug Resistance Plasmid Grant to Aid Cervical Cancer Screening in Ghana Additional...

  6. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...Cut-to-Mutation distance" affects editing efficiency. One important point to note is that Cas enzymes can continue...The size of the intended edit is the biggest determinant when selecting a type of donor. ssDNA templates...Easi-CRISPR for more details. References and resources Want to know more? Check out the additional resources...PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development...

  7. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ... monomers, although this can be prevented via antibiotic selection (Crozat, 2014). How to Detect Plasmid...CGE-LIF) for detection and can provide data on the quantity of various topologies and multimers present within...DH5α, HB101, NEB Stable, Stbl3, etc., are recA- mutant strains, and therefore should have reduced frequencies...plasmids causes instability: CoIE1 encodes a determinant essential for plasmid monomerization and stability...

  8. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...d microinject into blastocysts to be implanted in pseudopregnant female mice. If you obtained the desired...other homology-directed repair experiments, you’ll want the guide cut site to be as close as possible to...microinjection, i-GONAD is performed directly on pregnant female mice using a small incision that exposes...

  9. New Coomassie Protocol Video

    Type
    Blog Post
    ...your antibody's purity? Check out our latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies...

  10. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...For example, in bacteria, transposons can move antibiotic resistance genes between plasmids or from plasmids...is active in vitro as well as in vivo in yeast, plant, insect, and mammalian cells, including human cells...2021). Target site preference and host range are important to consider when selecting a transposon for a ... Sleeping Beauty and piggyBac, which is a big advantage over viral vectors (~5 kB payload for AAV and ...

  11. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...contrast, fluorescence imaging has emerged as the dominant light microscopy contrast technique in modern ...acquired all fluorophores throughout the sample are constantly exposed to light, not just those in the focal...for this to happen.Using red light has a second advantage: red light penetrates deeper into biological tissue...-time monitoring of neuronal activity across substantial 3D volumes. In addition to 2-photon microscopy...

  12. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...2!) decades that it's hard to summarize, but we wanted to mark the occasion by trying.  When we started...viral vectors, and in 2022 we added ready-to-use antibodies. Now, we think of ourselves as a repository for...keep an eye on our blog and social media. In the meantime, thank you all so much for being part of our community...

  13. Addgene at SfN23!

    Type
    Blog Post
    ...Addgene's booth? Well, first, Addgenies! We'll have an antibody specialist, Amrita, and a viral vector specialist...tons of information about Addgene's ready-to-use antibodies, viral vectors, and, of course, plasmids! Plus...Scientific Writing" on Sunday, from 12 pm - 2 pm. If you want to learn about, and practice, principles of clear...

  14. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    ...approximate, two-dimensional map. The Baker Lab wanted to create a new fold that is not present in nature...design model at atomic resolution” (Kuhlman and Dantas et al., 2003). That made it a powerful proof of...Hassabis, and John Jumper! References Kuhlman, B., Dantas, G., Ireton, G. C., Varani, G., Stoddard, B. L....

  15. Deck the Lab 2022!

    Type
    Blog Post
    ... my assay at like 23:00, so I decided to make a giant snow angel for biologists in our building. Unfortunately...2022   Congratulations to all our #DeckTheLab contestants!           View this post on Instagram                     ... labs with everything from Falcon tube trees to giant paper mache Krampus performing routine lab maintenance...

  16. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...also added AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO, deposited...offering AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO.  This is...bistable nature, it enables inhibition of synapses distant to the site of injection with high temporal precision...

  17. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ...depositor, but it can also be valuable data to share in grant proposals or to demonstrate impact in annual reports...features of dashboards are (i) easy access to important numbers and trends, (ii) user interaction with...metrics!  About the Author Joachim Goedhart is an assistant professor at the Section of Molecular Cytology...

  19. Editor's Choice, July 2016

    Type
    Blog Post
    ...peek in case you missed them. July 2016 was a fantastic month for the Addgene blog. We achieved roughly...editing when compared to the most commonly used Cas9 variant, SpCas9. These properties, combined with other ...in the post, may make Cpf1 the preferred CRISPR variant going forward.   Special Delivery Fluorophore Targeting...

  20. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    ...been working with Seeding Labs to provide plasmid grants to researchers in developing countries. Today we...announce that we’ve selected our first two plasmid grant awardees: Drs Louis Bengyella and Kwabena O. Duedu...excited to discover what new and creative ideas applicants will propose for plasmids from the Addgene community...
Showing: 781 - 800 of 1085 results