We narrowed to 970 results for: Abo;
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TypeBlog Post...first time AAV user Learn about how AAV is used in retinal gene therapy Learn about the parts of an AAV transfer...
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Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post... motif will be biotinylated. Find more details about RapID! EXCELL: Proximity labeling for the detection..., S. C., Noble, K. A., KC, B., Roux, K. H., Motamedchaboki, K., & Roux, K. J. (2016). An improved smaller... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post...PUF Assembly Kit Below you’ll find information about what these kits contain, how they can be used, and...encoded sensors enable real-time observation of metabolite production." Proceedings of the National Academy... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...colonies using one of the detection methods described above can help ensure the isolation of the monomeric version...plasmid DNA production platform incorporating low metabolic burden seed-stock and fed-batch fermentation processes... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...modifications. Like the TALE-LSD1 system described above, dCas9-LSD1 inactivates targeted enhancers. Although...setting up your experimental system. Let us know about your experience with these constructs in the comments... -
Antibodies 101: The Basics of Western Blotting
TypeBlog Post... the thickness of the band provides information about the relative abundance of the protein in the sample...history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog Post...transposase itself, please read on to learn more about this exciting technology from the folks at Transposagen...Additional Resources on the Addgene Blog Learn about the Sleeping Beauty Transposase Read up on Genome... -
Antibodies 101: Antibody Engineering and Directed Evolution
TypeBlog Post...structure-guided directed evolution. The nice thing about these methods is that they all follow the same general...performance. If you are interested in learning more about antibody engineering technologies, the Institute... -
Quick Guide to All Things Lentivirus
TypeBlog Post...first one and can be used routinely in a research laboratory. The risk of generating RCL is low but extra ...even your own! You should therefore be cautious about inserting potentially harmful genes into a transfer... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog Post...currently a research scientist in the Department of Laboratory Medicine at the University of Washington. She... Additional Resources on the Addgene Blog Read about Homology Directed Repair Use Knockout/Knockin Plasmids... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post...the most common concerns we get here at Addgene about this system is off-target or spurious expression...cells that don’t express Cre or Flp, what can we do about it? Test the serotype and promoter The first step... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog Post...FLEx enters the scene. FLEx: A light when night is about you! The FLEx (for flip-excision) switch (aka DIO...PMC4166436. Resources on the Addgene blog Learn more about FLEx vectors Download our Plasmids 101 eBook Read... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog Post...emitted, making it easier to capture a clear image above background. The majority of PA-FPs come in 2 flavours...Additional Resources on the Addgene Blog Read More about Fluorescent Protein Imaging Choosing Your Fluorescent... -
Typing CRISPR Systems
TypeBlog Post...Class 1 is the most abundant of the two, comprising about 90% of identified CRISPR-Cas in bacteria and nearly...CRISPR systems called CasPEDIA, with information about enzyme activity, experimental conditions, and more... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog Post...still isn’t over! Keep an eye out for our next post about base editing, where we’ll tackle transversion editors...CRISPR 101: Cytosine and Adenine Base Editors Read about four base editor reporters CRISPR 101: Prime Editing... -
Antibody Validation for Flow Cytometry
TypeBlog Post...leukocytes. The file shared also contains information about the labeling pattern of several cell lines using...Laflamme, C., Virk, H., NeuroSGC/YCharOS/EDDU Collaborative Group, & ABIF Consortium. (2024). A guide to... -
Delivery Methods for Generating iPSCs
TypeBlog Post...methods do you have experience using? Let us know about your iPSCs experiences in the comments below! References...Additional Resources on the Addgene Blog Learn about Methods to Reprogram Peripheral Blood Cells Check... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog Post...of the genome editing process. Read on to learn about the various light-controlled CRISPR tools available...reaching the amber stop codon, TAG. (To learn more about site-specific incorporation of amino acids using... -
New Antibody Animation!
TypeBlog Post...Ready to learn about antibodies? Our latest animation is here to help! Join Abi as they explain what ... -
New Antibodies in the Lab Animation!
TypeBlog Post...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...