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  1. CRISPR 101: Epigenetics and Editing the Epigenome

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    Blog Post
    ...modifications. Like the TALE-LSD1 system described above, dCas9-LSD1 inactivates targeted enhancers. Although...setting up your experimental system. Let us know about your experience with these constructs in the comments...

  2. Plasmids 101: Dimers and Multimers

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    Blog Post
    ...colonies using one of the detection methods described above can help ensure the isolation of the monomeric version...plasmid DNA production platform incorporating low metabolic burden seed-stock and fed-batch fermentation processes...

  3. Antibodies 101: The Basics of Western Blotting

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    Blog Post
    ... the thickness of the band provides information about the relative abundance of the protein in the sample...history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using...

  4. PiggyBac-ing Through the Genome Editing Field

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    Blog Post
    ...transposase itself, please read on to learn more about this exciting technology from the folks at Transposagen...Additional Resources on the Addgene Blog Learn about the Sleeping Beauty Transposase Read up on Genome...

  5. Quick Guide to All Things Lentivirus

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    Blog Post
    ...first one and can be used routinely in a research laboratory. The risk of generating RCL is low but extra ...even your own! You should therefore be cautious about inserting potentially harmful genes into a transfer...

  10. Typing CRISPR Systems

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    Blog Post
    ...Class 1 is the most abundant of the two, comprising about 90% of identified CRISPR-Cas in bacteria and nearly...CRISPR systems called CasPEDIA, with information about enzyme activity, experimental conditions, and more...

  11. CRISPR 101: Cytosine Transversion Editors

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    Blog Post
    ...still isn’t over! Keep an eye out for our next post about base editing, where we’ll tackle transversion editors...CRISPR 101: Cytosine and Adenine Base Editors Read about four base editor reporters CRISPR 101: Prime Editing...

  12. Antibody Validation for Flow Cytometry

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    Blog Post
    ...leukocytes. The file shared also contains information about the labeling pattern of several cell lines using...Laflamme, C., Virk, H., NeuroSGC/YCharOS/EDDU Collaborative Group, & ABIF Consortium. (2024). A guide to...

  13. Delivery Methods for Generating iPSCs

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    Blog Post
    ...methods do you have experience using? Let us know about your iPSCs experiences in the comments below! References...Additional Resources on the Addgene Blog Learn about Methods to Reprogram Peripheral Blood Cells Check...

  15. New Antibody Animation!

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    Blog Post
    ...Ready to learn about antibodies? Our latest animation is here to help! Join Abi as they explain what ...

  17. Plasmids 101 eBook 4th Edition

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    Blog Post
    ...take our word for it!  “When people want to learn about plasmids, they don’t go to Wikipedia; they go to...

  19. Antibodies 101 Series on TikTok

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    Blog Post
    ... is an IgE isotype?"  @addgene Ready to learn about antibodies? Our new antibodies animation has all...
Showing: 781 - 800 of 962 results