We narrowed to 1,078 results for: CAN

...buffeted by wind and waves and sometimes wonder if we can take another blow. Back in March, we never expected... when we need as much open science sharing as we can get. The effects of Addgene’s brief shut-down...excellence through whatever lies ahead this year. We can see signs of the storm passing. We’ve recently piloted...

...research to explore a variety of phenomena, and so too can it be used to teach a variety of science concepts...deeper understanding of scientific concepts than can be gained from lectures alone. Using plants as an...Resources on the Addgene Blog Learn how BioBuilder can bring Synthetic Biology into the classroom Engineering...Arabidopsis in the classroom With funding from the American Society of Plant Biologists and support from the...

...from tertiary study, the myriad trajectories ahead can be condensed into two discernible paths. The first...how to utilize a wide array of media in doing so—can prove invaluable in the pursuit of public engagement... misinformation in the wake of a global pandemic can make for a sore reminder of how far we have yet to...

...help us acquire a lot of those plasmids so that we can try many different things in parallel.” Alternating...painful and accompanied by migraines. Those attacks can be brought on by a number of stressors, including...work on Waardenburg syndrome, a rare disease that can cause hearing loss and changes in coloring of the...prone to developing metastatic melanomas and other cancers. Joshua Wang (bottom right), also of the Wistar...

... writing down specific examples and notes so you can get back to the issues if the conversation gets off...possible. If you wait too long, a small correction can become an unnecessarily big issue for you and for...What could I (manager) have done differently? What Can You Learn Going Forward: If you could plan this ...

...time to hone your communication skills. So, where can a graduate student or post-doc start? The good news...your science with the community. Some of them you can do today! 10 ways to share your science 1)   If you...consider how those stories are being told. Twitter can be a good way to follow news about particular subjects...

...interact with extracellular ligands, their ectodomains can extend and open, binding ligands with high affinity...function. With the right research reagents, researchers can measure this conformational change to investigate... cellular processes.   Figure 1: Integrins can shape into three conformations: (A) bent-closed, ...its open state, activating the receptor. Ligands can bind to integrins in their closed states (A) and ...laboratory techniques related to antibody discovery. You can find cell surface receptor biology talks on the IPI...kidney development. Despite their ubiquity and significance, these receptors have proved challenging to ...

...Every entry has an accompanying image so that you can see for yourself how the AAV performed in that experimental...we hope to mine the data to find patterns that we can share with you about promoters, serotypes, expression...

... as often as they can. Karissa Milbury is currently a PhD candidate at the BC Cancer Agency and University... April 22, 2017. While experiments (and editors) can move slowly, news about possible broad changes to...also studies energy markets in his spare time. You can follow him on twitter at @thompn4. New York City,...stars and the instruments we use to study them. You can follow her at alexandrazgreenbaum.wordpress.com or...supporters. I'm raising some little scientists (as you can see in the picture) and this march proved to be the...Within the Sciences Learn How Changing Labor Laws Can Effect Postdoc Wages ... breast cancer at the Goodman Cancer Research Centre at McGill University in Montreal, Canada.  Outside...

...resource-consumptive, since only wells with one cell can give rise to single clones, and thus many plates ...truncated pipet tips. Indeed, an experienced operator can pick 48 clones from a 10 cm dish in 15- 20 minutes... a fluorescent protein), fluorescence microscopy can theoretically be used to select specific wells from...peptide maturation in neural and endocrine cells. She can be emailed at [email protected].   Additional... secondary biological screens. For example, for cancer researchers, if growth properties in agarose are...

...anti-SARS-CoV-2 antibodies (Fig 1B). This approach can augment viral surveillance by enabling rapid assessment...particles (MPyV VLPs ). This artificial nanoparticle can protect proteins from unfavorable interactions in...more D-glucaric acid, suggesting that MPyV system can improve metabolic pathway yields by encapsulating...the population.  Because indel generation by Cas9 can occur at a steady rate, scientists found a way to...Mapping antibody escape in SARS-CoV-2 by mutational scanning By Seth Kasowitz As multiple antibody-based vaccines...therapeutics. Jesse Bloom’s lab at the Fred Hutchinson Cancer Research Center developed a pooled plasmid library... the antibody toolbox, they screened antibody candidates using a synthetic human scFV library and characterized...

...journal club. This can be a challengingly vague metric but there are a few things you can point to. Does ...advance notice for the first meeting so that people can set aside time in their schedules and read the paper...should read the paper ahead of time!). This notice can be shortened as the meeting settles in and people...

...proved to be quite easy to achieve…” - but I think we can all agree that it sounds less than ideal.  Unexpected...amenable to protein engineering. As a result, you can now find Myc1-9E10 in a variety of isotypes like ...refinement of this trusty tool doesn’t end there: you can also find anti-Myc [9E10] Fab fragments and single-chain...9E10’s accessibility and the variety of formats you can find it in likely mean it will remain an important... the N-terminal half of the antigen differs significantly between human and the other species, suggesting...

...looking to find one. Luckily, there are websites that can help: Antibody data repositories, which share validation... that you use? Let us know in the comments so we can add it to the table! And if you're interested in ...Laboratory Cancer Any Data repository From the Frederick National Library for Cancer Research ...

...nature of working in industry and how delegating can empower people Chapter 3: How Crystal advanced at...diseases, from heart failure to Alzheimer’s.  You can follow her on Twitter @ShihCrystal.     Special thanks...

...Two plasmids that can be used to make inexpensive 100 bp or 1 kb DNA molecular weight ladders were recently...interested in making your own DNA ladder? You can find the Tan Lab’s DNA ladder prep protocol here and ...worth of ladder. The Penn State ladders are significantly cheaper. When using a homemade plasmid prep ...increases the costs to $20-30 per ladder: still significantly cheaper than commercial ladders. Are you interested...

...handy tool for you.   With a few quick steps, you can use PubMed to see which articles contain plasmids...sure to check out the other “LinkOut” badges you can add and use your new PubMed account to set-up emails...

...challenges in NGS by developing technologies that can help unlock the potential of modern sequencing instruments...because the yield or quality of individual libraries can be influenced by the quantity or quality of input...normalization steps, measured in time and consumables, can be daunting for large numbers of samples. As mentioned...

...AAV we have released in the last few months. You can also browse our entire AAV inventory. Our new AAVs... AAV2, AAV5, AAV8, AAV9, and AAV Retrograde. You can also request the specific serotypes you need instead... exhibits the brightest resting fluorescence and can be used for imaging small neuronal processes (dendrites...

... Tetbow for neuronal labeling To use Tetbow, you can introduce the four Tetbow plasmids (Addgene #104102...2014) (Addgene #104106 - #104108) (Figure 3) that can be introduced using in utero electroporation. Then...website. We update the site regularly and hope Tetbow can help you in your research.     To see Tetbow in ...