We narrowed to 954 results for: Comp;

...end joining (alt-EJ). Inhibitors to essential components of both pathways have been shown to be efficacious... al., Nat Com).     Fig. 3: HDR events compete with NHEJ-mediated edits of small insertions and...

..., but it still points to a far larger and more complex problem endemic to biomedical research: pervasive...same reference genomes and libraries. Further complicating the issue is the long and ugly history of reporting...

...replication that can be packaged into replication-incompetent phage particles using a helper system. These ...antimicrobials combines them with both replication-competent phages and antibiotics. Phage therapy fell out...

...transcription. The HIS3 reporter system (e.g. the Gateway-compatible bait Y2H plasmid pEZY202  from Yu-Zhu Zhang’s...particular cell type, or a subset based on a cellular compartment or growth stage). A prey library can either be...

...roughly measures granularity, a proxy for nucleic complexity of the cell.  The sample will have been labeled...labeled using a panel of markers, which is comprised of antibodies conjugated to fluorescent markers and/...

...Purity Purity of AAV preparations is assayed by comparing the relative stoichiometric ratios of the viral...Absolute determination of single-stranded and self-complementary adeno-associated viral vector genome titers ...

...in mice is possible (Guy et al., 2007). They accomplished this using Cre-Lox mouse models in which the...Cre not only halted the neurological damage but completely reversed it, regardless of age or symptom severity...

...these people at the time, but I met some very accomplished scientists and some rising stars in the world...timetable for the Singletons in their bins.  The next component of Binning Singletons is when we bring all the...

... Note: hypoxanthine (shown) is the nucleobase component of the nucleoside inosine. Created with BioRender.com...relied on deaminase enzymes, either to directly accomplish the base edit or to create the substrate for ...

...persistence of residual viral material [6]. The complete removal of the residual viral particles and establishment... in the presence of these two factors are not completely understood, the addition of an shRNA for P53 ...

...is a terrible disease that is, unfortunately, completely preventable. Mainly caused by exposure to asbestos...non-resectable mesothelioma by the National Comprehensive Cancer Network, a coalition of the top 27 cancer...

...institutions doing? Institutions are now preparing to comply with the changes to the law on Dec 1st, according...centrally administered, which leads to many complications, including the inability of some institutions...

...varied codon preferences among organisms aren’t completely understood, but some possible reasons include...codon optimization algorithms and DNA synthesis companies such as IDT and GenScript host online codon optimization...

...ml before the media becomes cloudy. Mycoplasma compete with host cells for nutrients and can alter expression...al. outline a do it yourself PCR-based approach complete with freely available positive controls. Routinely...

...HDR), a distinct cellular DNA repair pathway. Accomplishing this usually involves simultaneously introducing...mutation. Here, the blocking-only templates will compete with the blocking/intended mutation templates resulting...

...membrane-impermeant and can only enter cells with compromised plasma membranes — i.e., dead cells — while live...which yields a much weaker signal on live cells compared to dead cells. As a result, the largely negative...

...enhanced brightness, expression, and sensitivity compared with earlier SF-iGluSnFR. Image from Aggarwal ...size and reduced sensitivity to pH and calcium compared to previous lactate biosensors, and its affinity...

...undetectable in the blood. But the virus doesn’t completely disappear; it just hides in latently infected...viral reservoirs, and CRISPR may be the way to accomplish this tough job! Kamel Khalili’s lab at Temple...

...enhancer regions (Mendenhall et al., 2013). By comparing gene activation when enhancers were active or ...increased gene activation at the targeted site compared to other Tet1 delivery systems. Transcriptional...

... domain structure. Glypicans are proteoglycans composed of a protein core, 2–4 glycosaminoglycans (GAG...). Figure 5: TurboCas protein labeling compared to immunoprecipitation. The top panel depicts ...