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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 967 results for: Cre

Showing: 781 - 800 of 967 results

  1. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...Plasmid A is the decreased expression of Gene X. Ergo the positive control(s) should decrease the expression...

  2. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...interest is toxic. To combat this, scientists have created synthetic promoters, which typically include some...with somewhat weak expression. lacIq mutation increases expression of the repressor 10x, thus tightening...

  3. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...completing the research will advance the field, create a new field, impact disease treatment, etc., again... His interests include the quality control of secretory proteins in the Endoplasmic Reticulum, optimization...

  4. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...A., Tao, Y. A., Duby, J. E., Steinbeck, B. J., McCreary, J., Pierce, S. E., … & Liu, D. R. (2024). Efficient...variety of DNA-targeting proteins, so the team created versions using dCas9, zinc finger proteins, and...

  6. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...immunofluorescence and indirect immunofluorescence work. Image created with BioRender.com   Indirect IF is a much ...with key considerations about each step. Image created with BioRender.com   The first couple of steps...

  7. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...fluorescent proteins (NIR FPs) and biosensors have been created recently. As developers of many of these probes..., A.A., Oliinyk, O.S. & Verkhusha, V.V. How to Increase Brightness of Near-Infrared Fluorescent Proteins...

  8. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ... S65T) in the original jellyfish GFP (AvGFP) to create enhanced GFP (EGFP), a variant with improved brightness...fluorescent probes, recent engineering efforts have increased the brightness of these genetically encoded light...

  9. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ... (Jambor et al., 2021) for more guidelines for creating clear and informative figures. Pro-tip! If you..., Wissink, E. M., & Weissgerber, T. L. (2021). Creating clear and informative image-based figures for ...

  10. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...proteins, use a higher percentage of acrylamide to increase migration resistance and improve separation. For...enhance resolution. In this setup, gels have an increasing range of acrylamide content from top to bottom...

  12. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ..., while REC includes most of the bridge helix. Created with BioRender.com.   Cas9 has two major lobes...guide RNA, dark blue; DNA, gray; PAM site, yellow. Created with BioRender.com.   The exact conformational...

  13. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...process. To deposit plasmids online, you will need to create an account with Addgene before starting the process...plasmids, and adding all the plasmids at once increases the likelihood that the plasmids are given sequential...

  15. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    ...impressive entries, but we have faith our community of creative scientists will deliver some fabulous photos again...

  16. CRISPR Guide Update

    Type
    Blog Post
    ...added new sections to share and describe some incredible new CRISPR technologies developed and widely ...

  17. The 12 Days of CRISPR

    Type
    Blog Post
    ...gave to me: CRISPR activation and interference screens for primary human T cells🎵 On the 3rd day of CRISPR...

  18. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...can't celebrate Addgene without recognizing our incredible depositors and requestors. Thank you all so much...

  20. We’re Going Viral This Week!

    Type
    Blog Post
    ...came out last year on sucrose supplementation to increase AAV2 yields and we’re eager to share more! Lastly...
Showing: 781 - 800 of 967 results