We narrowed to 963 results for: Cre
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TypeBlog Post...immunofluorescence and indirect immunofluorescence work. Image created with BioRender.com Indirect IF is a much ...with key considerations about each step. Image created with BioRender.com The first couple of steps...
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Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog Post...fluorescent proteins (NIR FPs) and biosensors have been created recently. As developers of many of these probes..., A.A., Oliinyk, O.S. & Verkhusha, V.V. How to Increase Brightness of Near-Infrared Fluorescent Proteins... -
Hot Plasmids: Summer 2024
TypeBlog Post...A., Tao, Y. A., Duby, J. E., Steinbeck, B. J., McCreary, J., Pierce, S. E., … & Liu, D. R. (2024). Efficient...variety of DNA-targeting proteins, so the team created versions using dCas9, zinc finger proteins, and... -
Tips for Getting a Faculty Position
TypeBlog Post...completing the research will advance the field, create a new field, impact disease treatment, etc., again... His interests include the quality control of secretory proteins in the Endoplasmic Reticulum, optimization... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post... (Jambor et al., 2021) for more guidelines for creating clear and informative figures. Pro-tip! If you..., Wissink, E. M., & Weissgerber, T. L. (2021). Creating clear and informative image-based figures for ... -
Antibodies 101: The Basics of Western Blotting
TypeBlog Post...proteins, use a higher percentage of acrylamide to increase migration resistance and improve separation. For...enhance resolution. In this setup, gels have an increasing range of acrylamide content from top to bottom... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post...antibody” can be used for downstream IP/ChIP assays. Creating epitope-tagged clonal cell lines remains a challenge...crRNA complementary to the template strand could increase knock-in efficiency.Note: Our TAG-IN tool can ... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post...process. To deposit plasmids online, you will need to create an account with Addgene before starting the process...plasmids, and adding all the plasmids at once increases the likelihood that the plasmids are given sequential... -
A Needle in a Base-Stack: Cas9 Structural Biology
TypeBlog Post..., while REC includes most of the bridge helix. Created with BioRender.com. Cas9 has two major lobes...guide RNA, dark blue; DNA, gray; PAM site, yellow. Created with BioRender.com. The exact conformational... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog Post... cancer and your continued work is necessary to create therapeutics to cure the cancer. Authorship: Publishing...translations of documents that are not in English. Create a Google Scholar account Sanitize your LinkedIn... -
'Tis the Season to #DeckTheLab
TypeBlog Post...impressive entries, but we have faith our community of creative scientists will deliver some fabulous photos again... -
CRISPR Guide Update
TypeBlog Post...added new sections to share and describe some incredible new CRISPR technologies developed and widely ... -
Addgene's 20th Anniversary Party
TypeBlog Post...can't celebrate Addgene without recognizing our incredible depositors and requestors. Thank you all so much... -
The 12 Days of CRISPR
TypeBlog Post...gave to me: CRISPR activation and interference screens for primary human T cells🎵 On the 3rd day of CRISPR... -
We’re Going Viral This Week!
TypeBlog Post...came out last year on sucrose supplementation to increase AAV2 yields and we’re eager to share more! Lastly... -
New FLIS Plasmid (April Fools 2022)
TypeBlog Post...interrogate many scientific questions. But these tiny creatures do tend to come with a rather astonishing ability... -
Overwhelmed? Take a Break With Our 5 Favorite Science Comics
TypeBlog Post... showed that a laughter program significantly decreased immunological markers of stress in adolescents... -
New Videos: Addgene Lab Tips
TypeBlog Post...Addgene Videos Page 5 Science Rap Videos and Their Creator: Tom McFadden Using Video to Share Your Science... -
Twenty Years of Sharing Science!
TypeBlog Post..., Korea, Japan, India, and Brazil. We've also created a robust support system for scientists using materials... -
Addgene’s GIPHY Page is Here to Help You Express Yourself (and Your Gene of Interest)
TypeBlog Post...answers! via GIPHY We’ve been working hard to create the most informative and entertaining protocol ...