We narrowed to 1,033 results for: ELL

... on how your virus of interest causes cancer, as well as therapeutic strategies to target it, than you...at an unfamiliar venue. Finding your poster board well ahead of time ensures you aren’t scrambling to tack...published papers or “hot topics,” so be sure to be well-read on the recent literature in your field. Take...know every answer – don’t hesitate to be honest and tell your audience just that. Explain what you do know...

...uses a small molecule, a tag, and the endogenous cellular degradation machinery to function. The dTAG is...tag and small molecule. It does work in mammalian cells, mice, and even yeast, though (we have a kit!). ...and complete degradation of proteins in mammalian cells. Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350...Tags Plasmids for Endogenous Gene Tagging in Human Cells Antibodies 101: Epitope Tags  ...

...is an excellent resource that has gained a lot of traction with researchers. It is an excellent way to... future career. If it wasn’t something I liked – well, I was used to experiments not working, so just ...programs and departments Graduate school was an excellent time to try new things, develop new skills, and...

...transformation efficiency of the strain: when asking the cell to perform multiple recombination events, all linear...linear fragments need to get into the same cell. For simple cloning requiring just one or two recombination...recombination events, homemade competent cells of 107 CFU/μg are perfectly fine, yet performing multiple modifications...modifications will require ultracompetent (109 CFU/μg) cells. Primer design Using specific primer design (Figure...

...his own biases--many of which will be shared by fellow scientists--but that doesn’t make his introduction...have outgoing personalities to supplement their intellectual prowess and scientific skills. This is an era...has been such a great fit for me!), the book was a well-executed overview that recontextualized some areas...career.  Acknowledgements: I would like to thank fellow Addgenies Joanne Kamens, Marcy Patrick, Aliyah ...

...using an electric charge to help the DNA enter the cells. Some samples require a reduced antibiotic concentration...concentration or need to be transformed into different cells than were originally requested. Most of our samples... Start by scanning the sample barcodes into the cells in the same order that they exist within the rack...storage process - from deposited DNA to transformed cells and finally to bacterial glycerol stocks that are...

...named) “nanopores” that are attached to the flow cell membrane and have an electrical current running ...faster turnaround time than short-read sequencing, as well as the ability to sequence an entire viral genome..., with significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly McQueary References ...Addgene The Power Behind NGS Plasmid Validation: seqWell Addgene’s Tips for Plasmid Quality Control Additional...

... be safely used to deliver a genetic payload to cells, and the complexity of effectively deploying them... can be to use. AAVs, which can target different cell types depending on their serotype, are particularly...amount of vectors available and number of requests tell only part of the story. Viral vectors are, after...experimental data in a standardized format, sharing how well a given viral vector worked — or didn’t work — in...

...designed to help us work in large formats, such as 96-well microplates, and thanks to our dedicated product...: Customizable to our specific needs Modern and well-supported, so we could focus on our development ... workflow would handle plasmid sequences on a 96-well plate basis, but for rapid testing and refinements...blog:  The Power Behind NGS Plasmid Validation: SeqwellHow to Keep a Lab Notebook for Bioinformatics AnalysesAAV...

...Many thanks to our guest blogger Gary McDowell! Gary S. McDowell is currently the Executive Director of...by Future of Research Executive Director, Gary McDowell. On Dec 1st, the threshold at which salaried workers...come under this ruling, regardless of visa or fellowship status, in addition to certain staff scientists...

...other journals and several funding agencies (e.g. Wellcome Trust, NIH Data Sharing Policies, etc.) and is...numerous objections and concerns. Perhaps least compelling of these were those from researchers who wanted...released, we will be in danger of having to deliver on well-meaning but impractical requirements imposed by ...should open up new opportunities for research as well as increasing transparency. Thank you to our guest...

... The loading control is supposed to control for, well, loading errors. When using it to normalize your...Most housekeeping proteins are highly expressed in cells. This means that at sample concentrations appropriate...assay. This is influenced by sample concentration as well as by antibody dilution — both factors we’ve covered... C, indicating that β-actin is less sensitive as well as less linear than the total protein quantification...

...academia Require that postdoc salaries be paid on fellowships instead of grants such that PIs no longer save...brought in editors from a variety of publications as well as young PIs and all gave advice on how to succeed...Rafael Luna, Joan Reede, Alberto Roca, and Jessica Tytell made it was obvious that more data is needed for...discluded/not treated equitably even when hired; well-meaning mentors often fail to judge the work of ...

...Every time I asked a question, I felt like I was yelling. I imagined 6 years of biting my tongue and gratefully...lab across the hall or in the same department as well. What do they have to say about this lab’s reputation...appointments with group members or are they frequently cancelled? Choosing a role model Make sure the mentors ...target up to fail, shifting goal posts without telling the target Warning signs while interviewing: Lab...

...mismatched) around the ligation junction in 16 wells of a 96-well plate, allowing 6 conditions to be screened... (2006): 687-699. PubMed PMID: 16464020.  3. Ellenberger, Tom, and Alan E. Tomkinson. "Eukaryotic DNA ...ligase bound to nicked DNA-adenylate." Molecular cell26.2 (2007): 257-271. PubMed PMID: 17466627. 25. Luo...

...function using transcriptional recording sentinel cells! 🎵 On the 11th day of CRISPR, Addgene gave to me...Enhanced prime editing systems by manipulating cellular determinants of editing outcomes🎵 On the 5th ...activation and interference screens for primary human T cells🎵 On the 3rd day of CRISPR, Addgene gave to me: ...

...ImagingResearchers: Andrea Radtke, Ziv YanevCellosaurus: A Cell Line Information DatabaseResearcher: Amos...Undergraduate Research Experiences (CUREs)Author: Jason PellettieriNew Acoustic Reporter Genes: Ultrasound Imaging...

...Protein Plasmids 101: Visualizing Subcellular Structures and Organelles Resources at Addgene.org Find ...Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization ...

...multiplex gene editing: Guest blogger Qichen Yuan tells us about a new tool for efficient multiplex base...Demethylation: Guest blogger Daniel M. Sapozhnikov tells us how to use dCas9 (with no modifications!) as ...research story, I couldn't resist adding in this excellent Q&A from Tim Miles on everything from AAV prep...

...next week - be sure to come by booth #851 and say hello! Just in time for this meeting, Addgene is launching...synthetic biology, gene cloning and expression, as well as guide pages for Synthetic Biology and Bacterial...of a useful plasmid that’s not in the collection? Tell us about it with our Suggest a Plasmid Form! Finally...