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We narrowed to 809 results for: Eng

Showing: 781 - 800 of 809 results

  14. Deep Dive: qPCR

    Type
    Blog Post
    ...channels - helpful if you forget to set the channel wavelength before you run the plate.    Analyzing There...

  15. CRISPR Guide

    Type
    Guide
    ...rapidly became the most popular genome engineering approach. Engineered CRISPR systems contain two components...modified versions engineered to increase specificity and nuclease activity available. Engineered Cas9s have ...Xie, H., Liu, D., Mo, H., Zhou, R., Lang, B., Meng, B., Weng, H., & Hu, Z. (2022). FrCas9 is a CRISPR/Cas9...scientists have modified for a wide range of genome engineering techniques . Collectively, these techniques are...R epeat) technologies. Before CRISPR, genome engineering approaches like zinc finger nucleases (ZFNs) ...mutation within the targeted gene. However, the strength of the knockout phenotype for a given mutant cell... the NHEJ repair mechanism. Multiplex Genome Engineering Many CRISPR experiments involve editing more ...

  16. Modular Cloning Guide

    Type
    Guide
    ...plants. GreenGate 2.0 Toolkit Plant Expression Alexis Maizel 68 plasmids to extend the GreenGate Cloning...Genome Engineering, CRISPR, Bacterial Expression Anke Becker Plasmids to perform genome engineering in Vibrio...Vibrio natriegens . TALE Toolbox Genome Engineering, TALEN Feng Zhang Plasmids for building TALE nucleases...applications—like building synthetic genetic circuits, engineering metabolic pathways, assembling gene-editing tools...MoClo Plant Parts III: Transformation & Genome Engineering Kit Plant Expression, CRISPR Daniel Voytas 93...regulatory elements, coding sequences, CRISPR parts GreenGate Cloning System Plant Expression Jan Lohmann 56...Wayne Parrott 48 plasmids compatible with the GreenGate Cloning System (Lohmann) to create plasmids with...

  17. Optogenetics Guide

    Type
    Guide
    ...of optogenetics integrates optics and genetic engineering to measure and manipulate cells (frequently neurons...red dots). When exposed to light of the correct wavelength, the pore opens, cations flow into the cell (...channels or pumps that absorb light at specific wavelengths. Upon activation by light, these channels and...occurring microbial opsins that respond to different wavelengths of light, like blue or yellow light. These various...proton efflux. Researchers have used genetic engineering to improve these natural opsins - by inducing...the neuron. NpHR Variants. Variants have been engineered with enhancements such as: Human codon optimization...blue-green light. Mac Variants. Mac variants have been engineered to include enhancements such as: Improved photocurrent...

  18. Chemogenetics Guide

    Type
    Guide
    ...tools are unresponsive to native ligands and are engineered to respond to small molecules that do not affect...Designer Drugs (DREADDs), like RASSLs, are based on engineered G-protein coupled receptors, but unlike RASSLs...Ligands Muscarinic-receptor based DREADDs were engineered to respond to nM concentrations of clozapine ...Actuator Modules (PSAMs, pronounced SAMs). PSAMs are engineered α7 nicotinic acetylcholine receptor (nAChR) domains...clinically approved drug, the PSAM domain was engineered to respond specifically to the anti-smoking drug...through viral injection or use of genetically engineered mouse models. For viral injection, Adeno-associated...plasmids are available. Additionally, genetically engineered animal models expressing hM3Dq, hM4Di, and rM3D...

  19. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...applications. Other Engineered Variants Hybrid capsids Hybrid capsids are engineered capsids derived from...have been developed and successfully employed to engineer new rAAV capsid variants, such as systemic capsids.... A systemic capsid is a capsid that has been engineered to have enhanced function after intravenous administration...) Directed evolution has also been employed to engineer an rAAV serotype variant able to display neuronal... soma) transport. Many rAAV vectors have been engineered to express fluorescent proteins, often under ...) and a gRNA together into an rAAV capsid is challenging due to its limited packaging capacity, especially... such as SaCas9 from Staphylococcus aureus . The Feng Zhang lab has developed plasmids containing SaCas9...

  20. Molecular Biology Reference

    Type
    Guide
    ...proteins are transcribed and processed into mature messenger RNA (mRNA), which is then translated into proteins...and other genetic elements using specifically engineered plasmids. These plasmids have become possibly...to a certain species, tissue, or cell type. The strength of the promoter can control the level of insert... of short RNAs. Mammalian shRNA Tools Genome Engineering Plasmids Used to target and edit genomes. Genome...Recombinases , TALEN Plasmids and Kits , Genome Engineering Kits Reporter Plasmids Used for studying the ...Strain Vendor(s) Genotype BL21 Invitrogen; New England BioLabs E. coli B F dcm ompT hsdS(rB mB) gal ccdB...proAB) [F traΔ36 proAB lacIqZΔM15] NEB Stable New England Biolabs F' proA+B+ lacIq ∆(lacZ)M15 zzf::Tn10 (...
Showing: 781 - 800 of 809 results