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  1. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ... to admit to inviting interesting friends and colleagues to events for this purpose. If possible, make... more than 40 minutes for the panel discussion leaving 20 minutes for audience questions (30 if discussion...

  2. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...applications that don’t yet exist, but it wasn’t clear that this was even possible. There is a variety ...that MagLOV’s “astonishing” magnetoresponse was clearly visible whether expressed in E. coli, in mammalian...

  3. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...cell level as they are in vitro. Factors like photobleaching, lack of red-shifted indicators, and brightness...selectively is especially essential. If you want to learn more about how viruses can be used for chemigenetics...

  4. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...appear as opaque dots (colonies should contain at least 50 cells and be well-separated. If the growth rate...to a 96-well assay plate. We generally freeze at least 2 ampules each of 4 clones with different levels...

  5. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...used to: Monitor dynamics of vesicle fusion and release from the plasma membrane by fusing various vesicle...PMC4831023. Additional Resources on the Addgene Blog Learn More About Fluorescent Protein Aggregation Catch...

  7. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...page.  A new system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage...AAV5. AAV9 preps and more AAV1 versions will be released soon. Visualize axonal projections and putative...

  10. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...temperature). Photostability: Fluorescent molecules gets bleached (i.e. lose the ability to emit light) after prolonged...imaging technologies to study gene expression, nuclear localization, and dynamics” Curr. Opin. Cell. Biol...

  11. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...called a standard. The ideal standard curve has at least five dilutions, with each step of the series diluted...good” is my standard curve? A poor standard curve leads to over or underestimation of an unknown sample’...

  12. MXS Chaining

    Type
    Blog Post
    ...TagBFP 399nm/ 456nm histone 2B (H2B) Chromatin 2 Cerulean 433nm/ 475nm Lyn-tag (derived from the tyrosine-protein...on the Addgene Blog Read about Gibson Assembly Learn About Other Plasmid Cloning Techniques Plasmids ...

  13. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...AAVs do not integrate in the host genome but still lead to long-term (>10 years) transgene expression in...development. As a result, Luk Vandenberghe and colleagues at MEEI co-founded a non-profit to help push ...

  17. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...target the CNS or the PNS, respectively.  Read on to learn more about these two new AAV capsids and how they... Blog Important considerations when using AAVs Learn more about using AAV as a tool for gene expression...

  18. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...After uncoating, reverse transcription follows the release of the two strands of viral RNA and of the viral...genome can direct the production, packaging, and release of progeny viral particles. And there you have ...

  19. CRISPR Antimicrobials

    Type
    Blog Post
    ...antimicrobials using efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed... Bikard, David, et al. “Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials.” ...

  20. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...embryogenesis (Figure 3). mScarlet-I3 also offered clearer signals due to reduced background signal when tested... gene. Lastly, curing the mCherry-sgRNA plasmid leaves an edited strain carrying Cas9 to allow the introduction...
Showing: 781 - 800 of 1011 results