We narrowed to 939 results for: OMP

1. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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   ...Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells...Tarlow, Branden D., Milton J. Finegold, and Markus Grompe. "Clonal tracing of Sox9+ liver progenitors in ...

2. Antibodies 101: Beyond Surface Labeling

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   ...membrane-impermeant and can only enter cells with compromised plasma membranes — i.e., dead cells — while live...which yields a much weaker signal on live cells compared to dead cells. As a result, the largely negative...

3. Hot Biosensors 2022: Year-End Roundup

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   ...enhanced brightness, expression, and sensitivity compared with earlier SF-iGluSnFR. Image from Aggarwal ...size and reduced sensitivity to pH and calcium compared to previous lactate biosensors, and its affinity...

4. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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   ...HDR), a distinct cellular DNA repair pathway. Accomplishing this usually involves simultaneously introducing...mutation. Here, the blocking-only templates will compete with the blocking/intended mutation templates resulting...

5. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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   ...undetectable in the blood. But the virus doesn’t completely disappear; it just hides in latently infected...viral reservoirs, and CRISPR may be the way to accomplish this tough job! Kamel Khalili’s lab at Temple...

6. CRISPR 101: Epigenetics and Editing the Epigenome

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   ...enhancer regions (Mendenhall et al., 2013). By comparing gene activation when enhancers were active or ...increased gene activation at the targeted site compared to other Tet1 delivery systems. Transcriptional...

7. Hot Plasmids: Spring 2025

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   ... domain structure. Glypicans are proteoglycans composed of a protein core, 2–4 glycosaminoglycans (GAG...). Figure 5: TurboCas protein labeling compared to immunoprecipitation. The top panel depicts ...

8. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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   ... cell types. Figure 1: Diagram of AAV components. Created with BioRender.com. AAV vectors ...Transduction of Airway Epithelial Cells in Mouse Lungs Compared to That of AAV2 Vectors. Journal of Virology, ...

9. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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   ...direct injection of a CRISPR/Cas9 ribonucleoprotein complex with linear DNA as repair templates which reduces...worms are perfectly viable at 15 ºC, yet exhibit complete embryonic lethality at 25 ºC. This method resulted...

10. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    ...sites are both recognized by Cre, but they are comprised of different sequences (loxp and lox2272) and ...essential that every new vector, whether it be a completely new transgene or merely a new lot, be thoroughly...

11. A Guide to Getting Started in Undergrad Research

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    ...experiences! These applications can be highly competitive, so asking for help from mentors and other students... Imposter: The Effect of Perceived Classroom Competition on the Daily Psychological Experiences of First-Generation...

12. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    ...parameter to consider is the contrast ratio- a comparison of PA-FP brightness before and after photoactivation...variants are more monomeric than mEos2 (mEos4b is completely monomeric) and display bright and photostable...

13. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    ...vitro Both in vitro and in vivo Epigenetic marks Complete removal to convert to iPSCs and re-establishment...in science communication and publishing after completing her postdoctoral training from Massachusetts ...

14. New Coomassie Protocol Video

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    ...    If this video is helpful, you can find our complete collection of protocol videos on the Addgene YouTube...

15. New Western Blot Protocol Video!

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    ...That's why we gave our written protocol a video companion! Follow along as Addgenie Meghan and Abi run a...

16. A Case Study of Addgene Bioinformatics

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    ...bioinformatics quest, which led us to Seqera Labs, a company that provides open source workflow orchestration...

17. Welcome to the Lab Bench! New Protocol Series Helps Trainees Get Started in the Lab

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    ...vectors, but what we lacked were videos guiding completely new members of the lab or those taking introductory...

18. CRISPR Guide Update

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    ...well as alternatives to Cas9. All of this is accompanied by new or refreshed figures made using BioRender.com...

19. It’s CRISPR Week at Addgene!

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    ...updating our CRISPR guide. Our CRISPR guide is a comprehensive resource that covers most things CRISPR - from...

20. The 12 Days of CRISPR: 2021

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    ... gave to me: efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs. On the sixth...