We narrowed to 1,024 results for: PLE;

...primary cellular receptor and sometimes one or multiple coreceptors, which may be present on different...and modifying capsid and genomic structures. For example, rAAV pseudotypes mix the genome of one AAV serotype... AAV8 AAV8 has high transduction efficiency in multiple tissues, rapid transgene expression, and can cross...

...are working with proto-oncogenes or with human samples that have not been tested for HIV.  If not using...must be chosen carefully. Often researchers want a simple set-up where the gene of interest is expressed ...experience in transfection, CaCl2 transfection is a simple and cheap technique that should work well. Quickly...

...cassette (Dickinson et al., 2015) made this step as simple as heat-shocking to induce the expression of a ...direct injection of a CRISPR/Cas9 ribonucleoprotein complex with linear DNA as repair templates which reduces...worms are perfectly viable at 15 ºC, yet exhibit complete embryonic lethality at 25 ºC. This method resulted...

... any gene of interest, one should require that multiple gRNAs of different sequences give rise to the ...but can often require difficult decisions. For example, would it be better to use a less-active gRNA that... in the interpretation of results by requiring multiple sequences targeting a gene to score in order to...

... viral DNA sequences and expression profile at multiple steps from plasmid amplification in bacteria to... set of injections into your animal model. For example, in a recent study by the Wickersham lab (Lavin...essential that every new vector, whether it be a completely new transgene or merely a new lot, be thoroughly...

...extracting the DNA from fossilized amber and supplementing it with the DNA of present-day frogs via genetic...template.  Each zinc finger recognizes a nucleotide triplet and when several ZFNs are strung together, it can...the ZFNs, but instead of recognizing nucleotide triplets, the components recognize individual nucleotides...

...CRISPR-based gene editing (see table below). For example, the far-red light-activated split-Cas9 (FAST) ...translocation of the protein BldD to the nucleus. To couple this light-dependent activity to transcription,...proteins by fusing Cas9 to photoreceptors. One prime example was from the lab of Michael Lin, who developed ...

... Denise Roche, to edit the translation. We are pleased to announce that CRISPR 101: Una herramienta de...repository. This incredible gift will allow more people to access helpful resources for their CRISPR experiments...

...Created in collaboration with Addgene writers High Complexity Golden Gate Assembly with NEB's DADExperts: Matthew...KarplusIBEX Knowledgebase: A Data Resource for Multiplex Tissue ImagingResearchers: Andrea Radtke, Ziv ...

...pm. If you want to learn about, and practice, principles of clear, accessible writing through the lens...Poster Session TipsHow to Make Friends and Meet People at a Scientific Conference New to SfN? Try A Novice's...

... AAV performed in that experimental setup. For example, this is an experiment using the chemogenetic tool...thank you for your contribution to open science.  Please note that the AAV Data Hub is a beta project, and...

...was designed simply to be stable and novel, the principles that created it have been applied to numerous...protein evolution. The Baker Lab has deposited multiple Top7 plasmids at Addgene, along with other tools...

...preps. For example, last year we published an article describing a sucrose supplementation strategy to...

...Sleigh bells, please! It's time to announce the winners of the 2022 #DeckTheLab contest!  and it's.....I was bored this weekend while waiting to take samples from my assay at like 23:00, so I decided to make...

...Blog is a great way to get your name out there! Completed blog posts are also great to include on CVs and... with and discuss your work with many different people. Recent Guest Writers Have Included: Wenning Qin...

...basics - diving into the ins and outs, tackling complex questions, and giving tips for consistently generating... with some behind-the-scenes peaks of Addgene’s people, culture, and workplace. Rumor has it that a few...

... (AAVrg), and volumes of 1 mL, 2.5 mL, or 5 mL. Please note that all orders will be shipped in 100 µL ...are a big deal, so if you have questions, we have plenty of information available for you to dig into. Check...

..., in vitro screens cannot faithfully model the complex spatial, cellular, metabolic, and cytokine microenvironment... cells, an important immune cell type, in more complex and physiological microenvironments. These screens...

...just starting in the lab, but they are exactly the people you should talk to if ruminating the trials and... 14:32 - 16:07 : What do you think drives other people to do science? 16:08 - 22:04 : How have the make-up...

...know-how to transcend national borders and help people around the world. As part of our own mission, we...Mentorship Program Learn How to Network and Meet People at Scientific Conferences Resources on Addgene.org...