10 Ways to Share Your Science!
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...necessarily bad news, but it is an argument for taking the time to hone your communication skills. So, where can...
We narrowed to 939 results for: TIM
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Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post...fast and plates should be used witin 1 month for optimal efficiency. Beware of satellite colonies! Carbenicillin... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog Post...arms race" between phages and prokaryotes; the ultimate victor will be the species who has the strongest... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog Post...manuscript. As a non-native English speaker, I sometimes miss the sensitivity to judge how aggressive my... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog Post...next 6 months, pending quality control. For an estimate on availability date or to be notified when a ... -
Fluorescent Biosensors for Measuring Autophagic Flux
TypeBlog Post...acting as an internal control. Autophagic flux is estimated by the GFP/RFP ratio: a decrease in GFP fluorescence... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog Post...eliminates the double-stranding step and reduces both the time and cost for AAV genome sequencing. Figure... -
Antibodies 101: Epitope Availability
TypeBlog Post...antibodies, which only recognize one epitope. (It can sometimes be an issue with polyclonal antibodies, so it'... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog Post...variety of natural products. Recently, this group optimized their initial system by using a stronger promoter... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog Post... B.1.617.2 (Delta variant) was not tested at the time of this study. Figure 1: Neutralization ... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post...I had to cut it very abruptly to stay within the time limit. pic.twitter.com/j6rhCCby2m — Barbara Di Ventura... -
Viral Vectors 101: Transductions
TypeBlog Post...design and construction steps are complete, it’s time to make virus! The constructed viral vector containing... -
10 Great Guest Posts We're Thankful For
TypeBlog Post...from the post and the associated publication. Optimizing Donor DNA for Enhanced CRISPR Genome Editing ... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog Post...reagents and will enable researchers everywhere.” The timing is ideal as the NIH’s BRAIN Initiative has been... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog Post...titer dose (Example: 1E13 GC/mL) and subject age at time of injection. How long post-injection did you wait... -
New and Upcoming Viral Vectors - December 2019
TypeBlog Post...localize opsins to the soma to avoid off target photostimulation of axons and dendrites on neighboring neurons... -
Even more elegant: Single injection CRISPR/Cas9 in C. elegans
TypeBlog Post...requires less than eight hours worth of hands-on time. Creating a modular, selection-based gene tagging... -
Another Pathway into Cells: iTOP
TypeBlog Post...Compared to CCP transduction, iTOP is at least four times more efficient in primary fibroblasts. iTOP also... -
New and Upcoming Viral Vectors - June 2019
TypeBlog Post...next 6 months, pending quality control. For an estimate on availability date or to be notified when a ... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...PubMed Central PMCID: PMC5728288. Schiller, Lara Timantra, et al. "Enhanced Production of Exosome-Associated...