Addgene

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Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

Type

Blog Post

...painful stimuli are processed by a network of excitatory and inhibitory neurons whose function and connectivity...
Antibodies 101: Polyclonal Antibodies

Type

Blog Post

...protein in low abundance or whose conformational state may change. If your protein might become slightly...
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

Type

Blog Post

...further tested the base editors by correcting a mutation that causes Niemann-Pick disease type C, directly...
Developing Lab Management Software for Biology

Type

Blog Post

... track the location, quality, growth, and legal status of thousands of plasmids a day, like we do at Addgene...
Gendered Innovations: Why Does Sex of the Cell Matter?

Type

Blog Post

...Gendered Innovations. This project: 1) develops state-of-the-art methods of sex and gender analysis and...
Plasmids 101: Modular Cloning

Type

Blog Post

... allows users to test a variety of different orientations and components within the system to maximize...
CRISPR in the Clinic

Type

Blog Post

...targeted to precisely edit with the goal of ‘fixing’ mutations or introducing a desired edit, but this particular...
Viral Vectors 101: Calcium Sensors

Type

Blog Post

...switches from green fluorescence to a red fluorescent state only by simultaneous (1) user-controlled illumination...
Antibodies 101: Choosing the Right Isotype

Type

Blog Post

...that require high affinity binding, such as immunoprecipitations. Your sample species should also be considered...
Plasmids for Endogenous Gene Tagging in Human Cells

Type

Blog Post

...it’s also good to make a few single base pair mutations in crRNA target sites found within the homology...
Adenoviral Vector Production and Troubleshooting

Type

Blog Post

...ratio for the purified virus should be ~1.3 Quantitative PCR: the number of viral DNA packaged in virions...
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

Type

Blog Post

... The team found that "all-in-one" Nanoblades facilitated HDR with either a ssDNA or dsDNA donor template...
A Look Back at One Year of Plasmid Sharing for COVID-19 Research

Type

Blog Post

...now circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited...
Hot Plasmids and Viral Preps - March 2021

Type

Blog Post

...vivo studies should always take DNA’s chromatin status into account (Fig 1a). The laboratory of Karmella...
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

Type

Blog Post

...without clearly testing whether a certain methylation state causes a condition. To understand mechanisms and...
5 Tips for Troubleshooting Viral Transductions

Type

Blog Post

... to a target cell by reducing the repulsive electrostatic forces between these two negatively-charged ...
Cpf1 Update: Comparison to Cas9 and NgAgo

Type

Blog Post

...sophisticated genome-wide analysis. At many of its computationally predicted off-target sites, Cpf1 does not mediate...
Plasmids 101: Terminators and PolyA signals

Type

Blog Post

...sequence conserved Rho-independent termination annotation for 90 bacterial elements. By Ppgardne at English...
How-to: 5 Steps to a Great Panel Discussion

Type

Blog Post

...use any slides for the event. Slides are for presentations, not discussions. I will rarely work with a ...
Hot Plasmids: Winter 2025

Type

Blog Post

... and subthreshold events, including miniature excitatory postsynaptic potentials (mEPSPs) (Hao and Lee...

Showing: 781 - 800 of 836 results

Results per page:

Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

Antibodies 101: Polyclonal Antibodies

Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

Developing Lab Management Software for Biology

Gendered Innovations: Why Does Sex of the Cell Matter?

Plasmids 101: Modular Cloning

CRISPR in the Clinic

Viral Vectors 101: Calcium Sensors

Antibodies 101: Choosing the Right Isotype

Plasmids for Endogenous Gene Tagging in Human Cells

Adenoviral Vector Production and Troubleshooting

Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

A Look Back at One Year of Plasmid Sharing for COVID-19 Research

Hot Plasmids and Viral Preps - March 2021

Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

5 Tips for Troubleshooting Viral Transductions

Cpf1 Update: Comparison to Cas9 and NgAgo

Plasmids 101: Terminators and PolyA signals

How-to: 5 Steps to a Great Panel Discussion

Hot Plasmids: Winter 2025

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