...of antibodies as tools allowed us to detect and capture proteins of interest to better study them in vivo...tagged protein rather than at the C-terminus. These features made this tag easier to use and more flexible ...will remain an important tool for the foreseeable future. These newer findings simply add to our understanding...

...available, you now face the challenge of trying to figure out what system might work best for your particular...vector reseals and other incorrect products. This feature biases the reaction in favor of the desired assembly...which one works best for you. Happy cloning (and be sure to deposit your finished constructs at Addgene!)...

...depending on the size of the conference). This made sure I didn’t zone out and kept me engaged throughout...If you’re presenting a poster at a conference, be sure to practice beforehand. Generally, two types of ...be on their website or the program booklet. Make sure you’re familiar with the policies before posting...

...heterozygote into a homozygote and ensuring that all future offspring inherit the endonuclease gene. As we ...address many pressing global problems in health, agriculture, and conservation. But please be aware that there...biosafety officer should be familiar with appropriate measures. Barriers should be a component of all gene drive...

...and Fultineer analyzed the gnomAD dataset, which captures a broader swath of human genetic diversity, to...Project: a global resource to map genomic diversity. Nature 604, 437–446 (2022). https://doi.org/10.1038/s41586...weaponization of genetics research by extremists. Nature 610, 444-447 (2022). doi: 10.1038/d41586-022-03252...

...vision in a canine model of childhood blindness. Nature Genetics 28, 92-95. PubMed PMID: 11326284. 4. Bennett...L., 2015. Gene Therapy returns to centre stage. Nature Vol 526, p.351. PubMed PMID: 26469046. 6. The Forever... Rev Virol. 2014 Nov;1(1):427-51. DOI: 10.1146/annurev-virology-031413-085355. PubMed PMID: 26958729.  ...

...range of attention spans, skill levels, and STEM exposure. If you expect a lot of kids, you’re going to ... we can make this fun activity relate to STEM.” Figure 1 shows the activities we had for my school’s Math...Perhaps you have young children and you want to make sure their STEM education is supplemented. A 2009 study...

... mean a world of mental relaxation. And, I’m not sure I should put this in print, but I actually let my... my daughter skip school a couple of times. Be sure to add time with your family, for yourself and for...just “do science” – we are scientists. We have to figure out a way to keep being ourselves and to be happy...

..., the envelope gene, and a polyA tail.   Figure 1: Lentiviral production uses three plasmids: (...in Medical Virology (Li et a., 2018).   Figure 2: To pseudotype with the SARS-CoV-2 spike protein..., Bieniasz PD, Jäger U, Porter CD, Friedman T, McClure MO, Weiss RA (1997) Sensitization of rhabdo-, lenti...

...and S. cerevisiae (Tuller et. al., 2010). Figure 1: An overview of peptide synthesis. A ribosome...as Hoogsten base pairs and wobble pairs.  Figure 2: (A) The codon wheel depicts the 64 codons that...PM, Li W-H (1987) The codon adaptation index-a measure of directional synonymous codon usage bias, and...

...The detectable signal from this reporter can be measured and quantified to determine the presence and quantity...lateral flow strip format. They published this in Nature Biotechnology. Like the SHERLOCK detection system...2020) CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020...

... that lack cookie monster are sterile due to a failure of primary spermatocytes to enter meiosis. Testis...Dominguez, Roberto, and Kenneth C. Holmes. "Actin structure and function." (2011). PubMed PMID: 21314430. ...segment number and polarity in Drosophila." Nature287.5785 (1980): 795-801. PubMed PMID: 9390559. Additional...

...Addgene. In vitro screening for the best gRNA Figure 1: Validation of double strand break (DSB) mediated...were fusing, you may have several mosaic mice. To figure out what alleles are present in these mice, you...favourite mammalian model organisms in the near future.  Many thanks to our guest blogger Samantha Young...

...behavioral tasks was an elevated plus maze that measures risk aversion/anxiety. The mouse can move freely..., learning, and memory. We’ll cover these in a  future blog post, so stay tuned!  References Alpár, ...rapid and long-lasting antidepressant effects." Nature communications 10.1 (2019): 223. PubMed PMID: 30644390...

...transduction was not detected. They also used an ELISA to measure binding of PHP.B to a soluble version of LY6A. ...to the central and peripheral nervous systems." Nature neuroscience 20.8 (2017): 1172. PubMed PMID: 28671695...for widespread gene transfer to the adult brain." Nature biotechnology 34.2 (2016): 204. PubMed PMID: 26829320...

...than the other (Sandomenico et al., 2020). Figure 1: Comparison between the IgG antibody and scFv...of panning and scFv characterization.    Figure 2: Phage display starts with a pooled library of...Bates A, Power CA (2019) David vs. Goliath: The Structure, Function, and Clinical Prospects of Antibody ...

... dsDNA breaks: following a cut, the error-prone nature of non-homologous end joining (NHEJ) often leads...database, which relies on CAGE-seq to directly capture the mRNA cap, provides the most accurate mapping...examined the ability to use sequence-based and other features to nominate gRNAs that are likely to be active...

...pipeline processing (Nextflow), cloud infrastructure, and secure collaboration.  In that post, we talked...

...accompanied by new or refreshed figures made using BioRender.com. Figure 1: PRIME editing Please...

...the first day of CRISPR, Addgene gave to me: a miniature CRISPR-Cas system for mammalian use. On the second...Addgene gave to me: programmed genome editing by a miniature CRISPR-Cas12f nuclease. On the eighth day of CRISPR...