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We narrowed to 1,035 results for: URE

Showing: 781 - 800 of 1035 results

  1. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...results you've measured should change established theory. So, what can you do to ensure that your skills...our website and social channels to apply for our future events. In addition to this annual event, graduate...

  2. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...won’t work if the fragment ends have secondary structure or too much sequence similarity. REPLACR-mutagenesis...expressing recombineering proteins Red/ET from a temperature-sensitive plasmid. Homologous recombination then...also well suited for insertions and deletions. The figure above shows guidelines for primer design; a desired...

  3. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    ...at all 1,500 or 1,600 transcription factors and figure out where they bind to the genome. We’ve been doing...other scientists quickly? Myers: We were hoping to figure out how to do this for many hundreds of transcription...as well. We would also like to put some social pressure on many other labs that may be doing something...

  4. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ... known as the resting potential (Vmem), can be measured as the imbalance of electrical charge between ...of the pHLuorin fluorescent protein. This GEVI features increased brightness and improved plasma membrane...Levin, Director of the Allen Discovery Center. The future of this field has a high potential for the advancement...

  6. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...These workshops illustrate the open and modular nature of the workshop content: different parts could ...for securing funding to support this effort. The future of reproducibility workshops and lessons learned.... Be on the lookout for upcoming workshops at future conferences this year. Workshops are confirmed ...

  7. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...molecules of the RNA backbone to increase stability.  Nature has evolved ways to prevent degradation of important...and rendered useless after less than a minute of exposure to the appropriate light. The photoactivatable...to engage a target sequence. Upon a brief light exposure (several seconds) the gRNA is released and the...

  8. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ..., with the expectation for major advances in agriculture, materials, chemicals, and medicine. As they’...and where all of it is likely to lead us in the future. Building circuits from CRISPRs Addgene: What...interactions. That’s relevant if you want to make sure you don’t target anywhere else, but for synthetic...

  11. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...Golden (Gate) pot (Pryor et al., 2022).     Figure 1: Workflow for high complexity Golden Gate Assemblies...cycling conditions (see Table 1).   Enzyme Temperature Incubation time/cycling Overhang length ...also optimized the thermocycler protocols and made sure that T4 ligase was the correct choice for the job...

  12. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...IgG2a antibody. When using a monoclonal antibody be sure to use a highly specific secondary antibody, rather... IgG2a>IgG2b>IgG1 (Manning 2012).      Figure 1: Broadly reactive versus subclass-specific antibodies...antibodies rather than generally reactive ones. And make sure to check for any reported cross-reactivity when ...

  13. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ... have reached labs in over 75 countries. Figure 1: SARS-CoV-2 proteins and other components. Image... the spike protein was developed based on 100 structure-guided spike designs and has improved protein ...Maynard JA, Finkelstein IJ, McLellan JS (2020) Structure-based design of prefusion-stabilized SARS-CoV-...

  14. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ... Level 1 TUs into multigene constructs. Figure 2: The three assembly levels of MoClo. Image courtesy...modification of these chemical products. The flexible nature of MoClo allows users to test a variety of different....pone.0016765.  Hahn, F., Korolev, A., Sanjurjo Loures, L. et al. A modular cloning toolkit for genome...

  15. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...including propensity for off-targets, secondary structures, etc., as well as the presence of a Protospacer...one of several Cas9s that recognize NG PAMs. The Nureki lab generated SpCas9-NG, a rationally engineered...lab sought to address the PAM problem through structure-guided engineering of the PAM-interacting domain...

  16. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...depending on the size of the conference). This made sure I didn’t zone out and kept me engaged throughout...If you’re presenting a poster at a conference, be sure to practice beforehand. Generally, two types of ...be on their website or the program booklet. Make sure you’re familiar with the policies before posting...

  17. Of Myc and Men

    Type
    Blog Post
    ...of antibodies as tools allowed us to detect and capture proteins of interest to better study them in vivo...tagged protein rather than at the C-terminus. These features made this tag easier to use and more flexible ...will remain an important tool for the foreseeable future. These newer findings simply add to our understanding...

  18. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ...available, you now face the challenge of trying to figure out what system might work best for your particular...vector reseals and other incorrect products. This feature biases the reaction in favor of the desired assembly...which one works best for you. Happy cloning (and be sure to deposit your finished constructs at Addgene!)...

  19. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...heterozygote into a homozygote and ensuring that all future offspring inherit the endonuclease gene. As we ...address many pressing global problems in health, agriculture, and conservation. But please be aware that there...biosafety officer should be familiar with appropriate measures. Barriers should be a component of all gene drive...

  20. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...range of attention spans, skill levels, and STEM exposure. If you expect a lot of kids, you’re going to ... we can make this fun activity relate to STEM.” Figure 1 shows the activities we had for my school’s Math...Perhaps you have young children and you want to make sure their STEM education is supplemented. A 2009 study...
Showing: 781 - 800 of 1035 results