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We narrowed to 1,091 results for: addgene

Showing: 781 - 800 of 1091 results

  1. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...name a few.   Find Many FRET-Based Biosensors on Addgene's Biosensor Pages   Recent innovations have demonstrated...PubMed PMID: 26210398. Additional Resources on the Addgene Blog Tips for using FRET in Your Experiments Learn...FRET Based Biosensors Additional Resources on Addgene.org Find FRET Pairs Check Out Fluorescent Protein...

  2. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...an extensive library of monoclonal antibodies; Addgene collaborates with these companies to create and...10.1371/journal.pbio.1002165 More resources on the Addgene blog Antibodies 101: ValidationSelecting the Right...

  3. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...that it may later be excised by Cre recombinase. Addgene has several great options: pLV hUbC-dCas9-T2A-GFP...References and resources:  Additional Resources on the Addgene blog CRISPR101: Editing the Epigenome  Mapping...the 4D Nucleome with CRISPR/Cas9 Resources at Addgene.org Read our CRISPR Guide to Epigenetics References...

  5. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...and Conjugates Resources on Addgene.org Addgene's Antibody GuideAddgene's collection of ready-to-use recombinant...early life.    Resources More resources on the Addgene blog Antibodies 101:Selecting the Right AntibodyAntibodies...

  7. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...AAV plasmids containing the active promoters at Addgene. Performance of synthetic promoter AAVs in mice... other tissues.  Additional resources on the Addgene blog Read more about using AAV for retinal gene...of our viral vector blog posts Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral...

  8. 6 Tips for Grant Writing

    Type
    Blog Post
    ... why they should give you money. Check out the Addgene Blog for Some Ways to Improve Your Science Communication...multiplex assays.   Additional Resources on the Addgene Blog Learn How to Become a Grant Writing Professional...

  11. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    ...acquire during their PhD and postdoctoral training. Addgene executive director Joanne Kamens has written a ...Further Reading Find more Career articles on the Addgene Blog Read Joanne Kamen's Management for Scientists...a 5-part blog on the topic:  http://blog.addgene.org/management-for-scientists-managing-vs-leading. The...

  14. Technical Design of a Western Blot

    Type
    Blog Post
    ...Antibodies Resources on Addgene.org Addgene's Western Blot Protocol Addgene's Western Blot Protocol Video...Looking for a western blot protocol? Check out Addgene's western blot protocol and protocol video!  Antibodies...Resources and references More resources on the Addgene blog Antibodies 101: The Basics of Western BlottingAntibodies... Good luck and happy blotting! Many thanks to Addgenies Ashley Waldron, Meghan Rego, and Amrita Rhodes...

  15. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...perform pha-1 co-conversion are available through Addgene. In 2019, Farboud et al. described another similar...To see more CRISPR tools for C. elegans, visit Addgene’s C. elegans CRISPR plasmids page. Future perspectives...about genome editing with CRISPRs? Check-out Addgene's CRISPR Resources Learn about gRNA design and...

  16. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...reduce your lab’s environmental impact, check out Addgene’s blog post on bringing sustainable practices for...relatively little work after the initial setup. (Addgene actually uses some of these — if you’re curious...Nyla Naim. Resources Additional resources on the Addgene blog Plasmid Preps: Different Purity, Different...: Easy Action Items Additional resources on addgene.org DNA Quantification Protocol Recovering Plasmid...

  17. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...Resources on Addgene.org Addgene's Antibody Guide Ready-to-use antibodies in the Addgene repository Antibody...sailing, and reading.   More resources on the Addgene blog Antibodies 101: Introduction to Gating in ...

  18. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...fluorescent microscopy technique for your study see Addgene’s blog post Which Fluorescence Microscopy Techniques...experiment and are unsure which to choose, check out Addgene’s article: Which Fluorescent Protein Should I Use.../s10616-011-9415-0 Additional resources on the Addgene blog Check out other methods using fluorescent...proteins Learn more about antibodies Resources on Addgene.org Visit the Fluorescent Protein Guide Find antibody...

  19. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...advancement. Andrew Hempstead is a Senior Scientist at Addgene.  Andrew's interests include genome engineering...PMCID: PMC5681539. Additional resources on the Addgene Blog Subscribe to CRISPR blog posts Learn about...techniques Find tips on gRNA design Resources on Addgene.org Browse all CRISPR plasmids Find validated gRNAs...

  20. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...many times and could be contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage...Second  Edition), 2012. Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian...Plasmids 101: Mammalian Vectors Resources on Addgene.org Find mammalian plasmids for your research Find...
Showing: 781 - 800 of 1091 results