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We narrowed to 1,090 results for: addgene

Showing: 781 - 800 of 1090 results
  1. Plasmids 101: 5 factors to help you choose the right cloning method

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    Blog Post
    ...be sure to deposit your finished constructs at Addgene!). References 1. Li, Chaokun, et al. "FastCloning...PMCID: PMC3207894.  Additional Resources on the Addgene Blog Read other Plasmids 101 blog posts New to...Download the Plasmids 101 eBook Resources on Addgene.org0 Check out protocols for plasmid cloning Find...
  2. dTAG - You're it!

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    Blog Post
    ...generated a series of plasmids that are available from Addgene. These plasmids enable lentiviral expression of...molecule: dTAG-47 Additional Resources on the Addgene Blog Easi-CRISPR: Generating Knock-In and Conditional...endogenous gene tagging in human cells Resources on Addgene.org Browse all CRISPR plasmids CRISPR plasmids for...
  3. Plasmids 101: Protein Expression

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    Blog Post
    ... use. Alyssa D. Cecchetelli is a Scientist at Addgene. She received her PhD from Northeastern University...proteins in plant root exudates Resources on the Addgene Blog Learn about inducible promoters Learn about...promoters Check out protein tags Resources on Addgene.org Bacterial expression systems Search for plasmids...
  4. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    Blog Post
    ...with your research? Plasmids are available from Addgene! References Schopp, I.M., Ramirez, C.C., Debeljak...Central PMCID: 5053326. Additional Resources on the Addgene Blog Learn about techniques for visualizing protein...-DNA Interactions with CUT&RUN Resources at Addgene.org Find plasmids from the Bethune Lab Find Plasmids...
  5. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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    Blog Post
    ...AAV plasmids containing the active promoters at Addgene. Performance of synthetic promoter AAVs in mice... other tissues.  Additional resources on the Addgene blog Read more about using AAV for retinal gene...of our viral vector blog posts Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral...
  6. Fluorescent Proteins 101: Introduction to FRET

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    Blog Post
    ...name a few.   Find Many FRET-Based Biosensors on Addgene's Biosensor Pages   Recent innovations have demonstrated...PubMed PMID: 26210398. Additional Resources on the Addgene Blog Tips for using FRET in Your Experiments Learn...FRET Based Biosensors Additional Resources on Addgene.org Find FRET Pairs Check Out Fluorescent Protein...
  7. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    Blog Post
    ... using Nanoblades? Plasmids are available from Addgene. Genome editing primary cells with Nanoblades Primary...PMCID: PMC 6318322. Additional resources on the Addgene blog Learn about RNP delivery Learn about CRISPR...Browse our CRISPR 101 blog post Resources on Addgene.org Check out our comprehensive CRISPR guide Find...
  8. Corralling and Cornering the Goldilocks Antibody

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    Blog Post
    ...an extensive library of monoclonal antibodies; Addgene collaborates with these companies to create and...10.1371/journal.pbio.1002165 More resources on the Addgene blog Antibodies 101: ValidationSelecting the Right...
  9. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    Blog Post
    ...that it may later be excised by Cre recombinase. Addgene has several great options: pLV hUbC-dCas9-T2A-GFP...References and resources:  Additional Resources on the Addgene blog CRISPR101: Editing the Epigenome  Mapping...the 4D Nucleome with CRISPR/Cas9 Resources at Addgene.org Read our CRISPR Guide to Epigenetics References...
  10. Antibodies 101: Conventional vs Spectral Flow Cytometry

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    Blog Post
    ...and Conjugates Resources on Addgene.org Addgene's Antibody GuideAddgene's collection of ready-to-use recombinant...early life.    Resources More resources on the Addgene blog Antibodies 101:Selecting the Right AntibodyAntibodies...
  11. 6 Tips for Grant Writing

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    Blog Post
    ... why they should give you money. Check out the Addgene Blog for Some Ways to Improve Your Science Communication...multiplex assays.   Additional Resources on the Addgene Blog Learn How to Become a Grant Writing Professional...
  12. Gaining Leadership Skills Volunteering at a Professional Organization

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    Blog Post
    ...acquire during their PhD and postdoctoral training. Addgene executive director Joanne Kamens has written a ...Further Reading Find more Career articles on the Addgene Blog Read Joanne Kamen's Management for Scientists...a 5-part blog on the topic:  http://blog.addgene.org/management-for-scientists-managing-vs-leading. The...
  13. Technical Design of a Western Blot

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    Blog Post
    ...Antibodies Resources on Addgene.org Addgene's Western Blot Protocol Addgene's Western Blot Protocol Video...Looking for a western blot protocol? Check out Addgene's western blot protocol and protocol video!  Antibodies...Resources and references More resources on the Addgene blog Antibodies 101: The Basics of Western BlottingAntibodies... Good luck and happy blotting! Many thanks to Addgenies Ashley Waldron, Meghan Rego, and Amrita Rhodes...
Showing: 781 - 800 of 1090 results