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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 956 results for: cha

Showing: 781 - 800 of 956 results

  1. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...destination vectors prior to recombination, and it is exchanged with the gene of interest during the BP or LR ...Multiple Hosts. Curr Protoc Protein Sci. 2003 Feb;Chapter 5:Unit 5.17. PubMed PMID:18429245. 3. Ptashne, ...

  2. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...Although different types of cancer have different challenges and obstacles to overcome, mesothelioma sufferers...of immunotherapy is the use of drugs (or other mechanisms) that work through body’s immune system to fight...

  4. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...My Bio 101 professor displayed a codon table — a chart every molecular biologist has seen before, showing...bacteria and eukaryotes. But be careful — antibiotic mechanisms vary! Aminoglycoside antibiotics also block protein...

  5. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...suited for recombinant protein expression. Many challenges can arise when over-expressing a foreign protein...minutes before adding the inducer. Growth Media: Changing media is tricky, because there can be a trade-...

  6. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...enzyme screening is sensitive to single base pair changes, but the edit must occur at an existing restriction...simultaneously validate edits and look for off-target changes. This method requires a set of control cells to...

  7. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ... This test can be performed in two steps for challenging samples as in Two-Step-SHERLOCK or in a fast,...the immobilised target sequences. Upon binding, changes in fluorescence can be visualised. Another approach...

  9. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...might suggest, an RNA-mediated gene silencing mechanism. RNAi was a landmark discovery in the 1990s and..., C. (2018). Overview of MicroRNA biogenesis, mechanisms of actions, and circulation. Frontiers in Endocrinology...

  10. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...ll notice that while the height of your events changes, the separation pattern of positive vs. negative...live population from my live-dead staining. (B) Changing the y-axis from SSC to FSC can alter the positioning...

  11. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...role of renaturation temperature in the polymerase chain reaction." Analytical Biochemistry 209.2 (1993):...Xia, Yongzhen, et al. "New insights into the QuikChangeTM process guide the use of Phusion DNA polymerase...

  12. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ... a well-established viral reservoir. A second challenge for CRISPR HIV-1 therapies is sequence specificity.... Ramanan V, Shlomai A, Cox DB, Schwartz RE, Michailidis E, Bhatta A, Scott DA, Zhang F, Rice CM, Bhatia...

  14. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...below outlines a few of the more common genetic changes found in E. coli strains. Table 1: Common gene ...are methylation sensitive. dnaJ Mutation in a chaperonin gene Increases the stability of certain expressed...

  15. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...the C’ terminus of the protein, to maximize the chances of creating a non-functional allele. For a 1 kilobase...the so-called base editor Cas9, which makes DNA changes in the absence of dsDNA breaks (Rees et al., 2018...

  16. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...were inspired by the imminent threat of climate change to study plant and marine biology, environmental...and while doing scientific research. It’s okay to change your mind about what exactly you want to do—I started...

  17. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...Watson, Crick, & Franklin, Meselson-Stahl, Hershey-Chase...Who was I to dispute history? And boy did I make...process that kits require; Nicole, the web master in charge of all our externally facing kit pages. No single...

  18. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ... using proper technique, there’s always a small chance that a researcher could infect him or herself with...you put in your culture. As viral production can change from one batch to another. For experimental consistency...

  19. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ... genomic DNA to amplify the gRNA targets was challenging because you’re limited by the sequence of the...Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE. Nat Biotechnol...

  20. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...Today's postdoctoral fellows (PFs) face a number of challenges ranging from long periods of training to limited...is equally important to have a plan to get to mechanism. Do the necessary tools exist to tackle the problem...
Showing: 781 - 800 of 956 results