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We narrowed to 951 results for: cha

Showing: 781 - 800 of 951 results

  1. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...enzyme screening is sensitive to single base pair changes, but the edit must occur at an existing restriction...simultaneously validate edits and look for off-target changes. This method requires a set of control cells to...

  2. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...might suggest, an RNA-mediated gene silencing mechanism. RNAi was a landmark discovery in the 1990s and..., C. (2018). Overview of MicroRNA biogenesis, mechanisms of actions, and circulation. Frontiers in Endocrinology...

  3. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ... This test can be performed in two steps for challenging samples as in Two-Step-SHERLOCK or in a fast,...the immobilised target sequences. Upon binding, changes in fluorescence can be visualised. Another approach...

  5. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...role of renaturation temperature in the polymerase chain reaction." Analytical Biochemistry 209.2 (1993):...Xia, Yongzhen, et al. "New insights into the QuikChangeTM process guide the use of Phusion DNA polymerase...

  6. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ... a well-established viral reservoir. A second challenge for CRISPR HIV-1 therapies is sequence specificity.... Ramanan V, Shlomai A, Cox DB, Schwartz RE, Michailidis E, Bhatta A, Scott DA, Zhang F, Rice CM, Bhatia...

  8. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...below outlines a few of the more common genetic changes found in E. coli strains. Table 1: Common gene ...are methylation sensitive. dnaJ Mutation in a chaperonin gene Increases the stability of certain expressed...

  9. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...the C’ terminus of the protein, to maximize the chances of creating a non-functional allele. For a 1 kilobase...the so-called base editor Cas9, which makes DNA changes in the absence of dsDNA breaks (Rees et al., 2018...

  10. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...Watson, Crick, & Franklin, Meselson-Stahl, Hershey-Chase...Who was I to dispute history? And boy did I make...process that kits require; Nicole, the web master in charge of all our externally facing kit pages. No single...

  11. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...Today's postdoctoral fellows (PFs) face a number of challenges ranging from long periods of training to limited...is equally important to have a plan to get to mechanism. Do the necessary tools exist to tackle the problem...

  12. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...were inspired by the imminent threat of climate change to study plant and marine biology, environmental...and while doing scientific research. It’s okay to change your mind about what exactly you want to do—I started...

  13. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ... using proper technique, there’s always a small chance that a researcher could infect him or herself with...you put in your culture. As viral production can change from one batch to another. For experimental consistency...

  14. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ... genomic DNA to amplify the gRNA targets was challenging because you’re limited by the sequence of the...Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE. Nat Biotechnol...

  16. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ... editing or labeling efficiency doesn’t usually change when you add multiple gRNAs on one plasmid. Sound...contains all of the desired gRNAs. This increases the chance that all the edits you want to make will happen...

  17. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ... is working for you, then there is no reason to change it. But what if a kit isn’t working for you, or...factor label Non-silica based, avoids use of chaotropic salts   What are all these other kit types...

  18. New Antibody Animation!

    Type
    Blog Post
    ...mammals.      Subscribe to Addgene's YouTube channel for more helpful animations, useful protocol videos...

  19. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    ...Haery has summed up some of the most important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses...

  20. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ...you can watch the full animation on our YouTube channel.  More resources on the Addgene blog Antibodies...
Showing: 781 - 800 of 951 results