We narrowed to 1,050 results for: ins
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TypeBlog Post...transcription and chimeric efforts make a precise edit or insertion via a chemical modifier. We used these categories... plasmids seem to never truly go out of style. Instead, an almost even mix of plasmids (by age) are requested...
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Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog Post... vector (deposited by David Root of the Broad Institute) has consistently been one of the repository’s...satisfaction of checking off each step as you painstakingly complete your experiment – one small victory... -
Summer SciComm Series: Modes of Communication
TypeBlog Post...information. Personally, I would much rather read the instructions than watch a how-to video. My dad, on the other...love it and others will avoid it like the plague. Instead of looking for The One Method to Rule Them All,... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog Post...time to produce beta-lactamase prior to division, instead of immediately experiencing the effects of the ... capable of correctly folding disulfide bonded proteins in its cytoplasm. Microbial Cell Factories, 11... -
A Guide to Starting Your Own Journal Club
TypeBlog Post...including casual request via email, in person, or instant messaging but we as scientists often prefer data...failing in many academic journal clubs where institutional inertia drives the meeting rather than interest... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog Post...Eleven years later, Addgene is a company of fifty instead of three, and we are sending hundreds of plasmid...yard this year, though she generously offered. Instead we picniced outside of our new office space on ... -
Negotiating Work and Life: How to Find the Joy
TypeBlog Post...run and the long run and so will your work. Nobody wins, and you need to make a change. #2 Thou shalt sometimes...tasks – try to resist the impulse to control by insisting things always be done your way. Be patient with... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog Post...open new ways for a novel therapeutic approach against drug addiction. Anxiety tests Rodents have a natural...are ahead for behavioral neuroscience to reveal insights into the complex relationship between genes, neurons... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...is not amenable to FACS. Thus, the researchers inserted human CD8, a gene that encodes a surface protein...yourself whether gene overexpression is necessary (instead of gene knockout or knockdown, for which there ... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...manipulated by injected RNA and can express fluorescent proteins. This lab has created an automated tank for feeding... fusion is such that the two plants share RNA, proteins, and DNA. Euprymna scolopes (Joshua Rosenthal... -
Lab to Office Culture Shock
TypeBlog Post...for scientists making this transition. In this installment of the Addgene career guide, we’ll think about... Eric J. Perkins... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog Post...Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F (2017) Nucleic acid detection with ...Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F (2018) Multiplexed and portable nucleic... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog Post...you can either order a DNA pooled library, which contains a collection of plasmids in a single tube, or ...lentiviral CRISPR libraries. Protocols from the Broad Institute- A variety of protocols and guides regarding pooled... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...circuit is sufficient to induce reinforcement (instills a sense of “reward”), then the animal will repeatedly...derived from direct comparative analysis of microbial opsins." Nature methods 9.2 (2012): 159-172.5. Goshen ... -
Finding Your Science Policy Path
TypeBlog Post...the mission so much that they will often move mountains to give advice and guidance to those that ask....great sources of volunteer opportunities. NSPN maintains a great resource guide including conferences, ... -
New Coomassie Protocol Video
TypeBlog Post...protocol can easily be adapted for other types of proteins. If this video is helpful, you can find our... -
New Antibodies in the Lab Animation!
TypeBlog Post...the Lab" shows how antibodies are used to study proteins via ELISAs, western blots, immunoprecipitation... -
Addgene Launches Partnership with IPI
TypeBlog Post...announce the launch of our partnership with the Institute for Protein Innovation (IPI). Together, we’ll ... -
Antibodies 101 Series on TikTok
TypeBlog Post...our videos will also be shared on our @addgene Instagram account, or you can watch the full animation on... -
#DeckTheLab to Win a Tasty Winter Treat This Holiday Season
TypeBlog Post...causing him some difficulty… To give him some inspiration, we’d love to see how you’re decorating your ...