...basics - diving into the ins and outs, tackling complex questions, and giving tips for consistently generating...

... me best?     But between these options, we simply can't decide! Abi, Lin, or Blottie? Which name ...

...pharmaceutical company based in Switzerland. After completing her PhD in Chemistry and Chemical Engineering...

...bacteria, fungi, and viruses. Final tree with the complete #MicrobeAdventCalendar ornament set (plus more...

... to the needs of the scientific community. For example, we started a new service distributing ready-to-use...

...useful in FRET experiments. Professor Fessenden's example of a FRET experiment with all relevent controls...

...for the presence of pathogens in environmental samples via PCR. We hope these plasmids will help Dr. Duedu...

...synthetic genome project and constructing ever more complicated biological devices. Chapter 6: - Making research...

... scientific environment 3:09 - 5:58 : Specific Examples where Niroshi and Ben helped build their grad ...

...working lab full of busy Addgenies preparing plasmid samples for shipping. This was the favorite part of their...

...measure will ever adequately capture a notion as complex as “scientific impact.” As we look ahead to the...

...Science Translational Medicine have made their complete collection of relevant papers freely available...

...the amplified sample, followed by reamplification of the DNA, but please note that NGS should be performed... Protocols CRISPR Library Amplification CRISPR Library Amplification You may also like... Pooled libraries...different genes in an organism's genome, for example. Amplification is usually necessary to produce sufficient... pre-amplified stock. A last option is to perform a gel extraction of either the original sample or the...Follow this protocol to perform amplification of CRISPR pooled plasmid libraries in Escherichia coli ...refer to our pooled library material pages for amplification protocols that have been developed by the depositor...available. If a pooled library does not yet have an amplification protocol, the following protocol can be used...

...specific paper. If you’re ready to start a deposit, simply click on “Deposit Materials” and the search bar...

...nucleic acid and chemical contaminants, these DNA samples must also be entirely endotoxin free. Endotoxin...

...available for a little over a year and a half, a small sampling of the research it has already been used for includes...

...engineer around the current limitations of the Cas9 complex," said study author F. Ann Ran, a graduate student...

...efficiency can be controlled by manipulating complementarity between the gRNA and target. Read the paper...

...Purification Without a Kit by Beth Kenkel To complement our RNA Extraction Without A Kit blog post, we've...

...after vegetables; Broccoli, Spinach, and Corn, for example. Learn more about the aptamer soup in this blog...